番茄潜叶蛾幼虫的龄数和龄期测定

doi:10.16409/j.cnki.2095-039x.2022.03.024

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (2): 340-345.DOI: 10.16409/j.cnki.2095-039x.2022.03.024

番茄潜叶蛾幼虫的龄数和龄期测定

张桂芬^1,2, 殷惠军¹, 王玉生^1,3, 李振江¹, 毕思言¹, 王瑞^1,2, 刘万学^1,2, 万方浩^1,2

1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室/农业农村部外来入侵生物防控重点实验室, 北京 100193;
2. 农业农村部外来入侵生物预防与控制研究中心, 北京 100193;
3. 湖南农业大学植物保护学院, 长沙 410125

收稿日期:2022-03-06 发布日期:2023-04-13
通讯作者: 张桂芬
作者简介:张桂芬,博士,研究员, E-mail:zhangguifen@caas.cn;殷惠军,硕士研究生, E-mail:2221651519@qq.com。
基金资助:
国家重点研发计划（2021YFD1400200，2017YFC1200600）；中国农业科学院科技创新工程（caascx-2017-2022-IAS）；长沙市自然科学基金（kq2014067）

Determination of Larval Instars and Duration of the Tomato Leaf miner, Tuta absoluta (Meyrick) (Lepidoptera:Gelechiidae)

ZHANG Guifen^1,2, YIN Huijun¹, WANG Yusheng^1,3, LI Zhenjiang¹, BI Siyan¹, WANG Rui^1,2, LIU Wanxue^1,2, WAN Fanghao^1,2

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Invasive Alien Species Management, Ministry of Agriculture and Rural Affairs/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Center for Management of Invasive Alien Species, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China;
3. College of Plant Protection, Hunan Agricultural University, Changsha 410125, China

Received:2022-03-06 Published:2023-04-13

摘要/Abstract

摘要： 番茄潜叶蛾Tuta absoluta (Meyrick) 源产于南美洲的秘鲁，是世界番茄的毁灭性害虫，2017年8月首次在我国新疆发现，对我国番茄及其他茄科粮蔬作物生产安全构成了潜在巨大威胁。龄期划分是深入研究番茄潜叶蛾生物学习性，明确其种群发生规律，提高预测预报和防治（如基于幼虫寄生蜂利用的生物防治）效果的基础。头壳宽度是幼虫分龄的重要指标。本研究通过逐日测量幼虫的头壳宽度和虫体长度，并运用频次分布、Dyar法则、Crosby指数、Brook指数和回归分析对测量的数据进行统计分析，研究番茄潜叶蛾的幼虫龄期划分。研究结果表明，番茄潜叶蛾幼虫头壳宽度符合Dyar法则和Crosby生长法则，并呈现出明显的指数回归关系，可作为幼虫龄期划分的重要形态指标。番茄潜叶蛾幼虫划分为4个龄期，各龄期幼虫头壳宽度分别为0.1375~0.1575 mm、0.2125~0.2725 mm、0.3200~0.4100 mm和0.4975~0.5800 mm。与之对应龄期的幼虫体长分别为0.80~1.52 mm、0.91~3.33 mm、2.42~4.89 mm和4.18~7.32 mm，可作为判定龄期数的辅助指标。于室内27 ℃温度下饲养幼虫，平均历期约13 d，其中1~4 d为第1龄、3~6 d为第2龄、4~7 d为第3龄、6~15 d为第4龄。头壳宽度可作为幼虫龄期划分的重要指标，番茄潜叶蛾幼虫分为4个龄期；同一龄期内幼虫体长变异性较大，各龄期重叠程度较高，可作为幼虫龄期划分的辅助指标。

关键词: 番茄潜叶蛾, 幼虫龄期, 头壳宽度, 回归分析, 体长

Abstract: The tomato leafminer Tuta absoluta (Meyrick), originated in Peru, South America, is a worldwide destructive pest of tomato. In China, T. absoluta was first detected in Xinjiang in 2017 and has posed a potential threat to China’s tomato and other grain and vegetable solanaceous crops. Division of larval instars of the leafminer is important for studies of biological characteristics, population dynamics, and population monitoring and prediction and control, such as biocontrol based on larval parasitoids. The head capsule width is an important index for larval instar division. The number of larval instars was determined and tested according to the frequency distribution analysis, Dyar’s rule, Crosby’s growth rule, as well as regressive analysis based on two measured parameters, the head capsule width and body length. The head capsule width of the leafminer larvae fitted well with the Dyar’s rule and Crosby’s growth rule. There was an obvious exponential regression relationship between the number of larval instars and the head capsule width, showing that the head capsule width can be used as an important index for larval instar division. The larvae of T. absoluta can be divided into four instars. The values of the head capsule width of the 1^st, 2^nd, 3^rd, and 4^th instar larvae were 0.1375―0.1575 mm, 0.2125―0.2725 mm, 0.3200―0.4100 mm, and 0.4975―0.5800 mm, respectively. The values of body length corresponding to the above four larval instars were 0.80―1.52 mm, 0.91―3.33 mm, 2.42―4.89 mm, and 4.18―7.32 mm, respectively, which could be used as an auxiliary index to determine the number of larval instars of the tomato leafminer. At 27℃, the larvae completed development in 13 d, the 1^st, 2^nd, 3^rd, and 4^th larval instars lasted 1―4 d, 3―6 d, 4―7 d, and 6―15 d, respectively. Thus, the T. absoluta larvae are divided into four instars according to the head capsule width, while the body length can be used as an auxiliary index in larval instar division duo to its great variation in the same and between instars.

Key words: Tuta absoluta, larval instar, head capsule width, regressive analysis, body length

中图分类号:

S476

张桂芬, 殷惠军, 王玉生, 李振江, 毕思言, 王瑞, 刘万学, 万方浩. 番茄潜叶蛾幼虫的龄数和龄期测定[J]. 中国生物防治学报, 2023, 39(2): 340-345.

ZHANG Guifen, YIN Huijun, WANG Yusheng, LI Zhenjiang, BI Siyan, WANG Rui, LIU Wanxue, WAN Fanghao. Determination of Larval Instars and Duration of the Tomato Leaf miner, Tuta absoluta (Meyrick) (Lepidoptera:Gelechiidae)[J]. Chinese Journal of Biological Control, 2023, 39(2): 340-345.

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番茄潜叶蛾幼虫的龄数和龄期测定

Determination of Larval Instars and Duration of the Tomato Leaf miner, Tuta absoluta (Meyrick) (Lepidoptera:Gelechiidae)

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