孤雌产雌松毛虫赤眼蜂种群间生殖表型的比较

doi:10.16409/j.cnki.2095-039x.2022.03.031

摘要/Abstract

摘要： 为明确温度、遗传背景和雌蜂日龄对孤雌产雌松毛虫赤眼蜂生殖表型和Wolbachia滴度的影响，在22 ℃和29 ℃下处理两种遗传背景（彰武、西丰）的孤雌生殖松毛虫赤眼蜂，逐日统计子代蜂生殖表型指标（雄性比，间体率）、适合度指标（日产卵量，羽化率）和Wolbachia滴度。结果发现，29 ℃下子代蜂雄性比和间体率显著高于22 ℃处理的子代蜂，但29 ℃下雌蜂体内Wolbachia滴度和日产卵量均显著低于22 ℃处理的雌蜂。彰武种群雄性比显著高于西丰种群，但其日产卵量显著低于西丰种群。随母代蜂日龄增加，子代蜂雄性比和间体率均上升，但日产卵量和羽化率均下降。结果表明温度、遗传背景和日龄均影响孤雌产雌松毛虫赤眼蜂生殖表型。研究结果将为改善孤雌产雌赤眼蜂规模化繁育和田间应用提供参考。

关键词: 沃尔巴克氏体, 松毛虫赤眼蜂, 孤雌产雌生殖, 遗传背景

Abstract: The effects of temperature, genetic background, and age on the reproductive phenotype and Wolbachia titer of Wolbachia-infected thelytokous line of Trichogramma dendrolimi Matsumura were investigated by analyzing the parameters of reproductive phenotype (male proportion and intersex proportion), fitness (numbers of parasitized host eggs and emergence proportion), and Wolbachia titer as influenced by temperature (22 ℃ and 29 ℃), female age, and genetic background (ZW line and XF line). The results showed that the male proportion and intersex proportion at 29 ℃ were significantly higher than those at 22 ℃. However, the Wolbachia titer and the number of daily parasitized host eggs deposited by maternal females at 29 ℃ were significantly lower than those at 22 ℃. The male proportion of ZW line was significantly higher than that of XF line, but the number of daily parasitized host eggs of ZW line was significantly lower than that of XF line. The male proportion and intersex proportion of offspring increased significantly with the age of maternal females, but the number of daily parasitized host eggs and emergence proportion of offspring decreased significantly with the age of maternal females. The results highlight the significant effects of temperature and age on the reproductive phenotype of Wolbachia-infected thelytokous line of Trichogramma dendrolimi Matsumura with different genetic background. The research is expected to help the improvement of the mass-rearing procedure and field application of thelytokous Trichogramma in biological control programs.

Key words: Wolbachia, Trichogramma dendrolimi, thelytokous parthenogernesis, gentic background

中图分类号:

S476.3

吕琳, 李媛媛, 霍梁霄, 梁洪宇, 张欢欢, 周金成, 张礼生, 董辉. 孤雌产雌松毛虫赤眼蜂种群间生殖表型的比较[J]. 中国生物防治学报, 2023, 39(2): 245-253.

Lü Lin, LI Yuanyuan, HUO Liangxiao, LIANG Hongyu, ZHANG Huanhuan, ZHOU Jincheng, ZHANG Lisheng, DONG Hui. Reproductive Phenotype of Two Wolbachia-infected Thelytokous Line of Trichogramma dendrolimi Matsumura[J]. Chinese Journal of Biological Control, 2023, 39(2): 245-253.

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