贝莱斯芽孢杆菌YJ02复配噻呋酰胺对花生白绢病增效防治作用

doi:10.16409/j.cnki.2095-039x.2026.02.010

摘要/Abstract

摘要： 为提高生防菌贝莱斯芽孢杆菌YJ02对花生白绢病的防治效果，本研究以花生白绢病菌齐整小核菌为靶标菌，探究生防细菌贝莱斯芽孢杆菌YJ02与化学杀菌剂复配防治花生白绢病的可行性。采用稀释涂布法和菌落计数法测定9种防治花生白绢病的化学杀菌剂对贝莱斯芽孢杆菌YJ02的生物相容性，含毒介质法测定相容化学杀菌剂及贝莱斯芽孢杆菌YJ02对齐整小核菌的室内毒力，通过毒性比率筛选出相容杀菌剂与贝莱斯芽孢杆菌YJ02复配的最佳比例，盆栽和大田试验评价筛选出的复配组合对花生白绢病的增效防治作用。结果表明，杀菌剂噻呋酰胺、嘧菌酯和肟菌酯均与生防菌YJ02具有良好生物相容性，以噻呋酰胺对花生白绢病菌毒力最高；1×10² CFU/mL YJ02与0.1 mg/L噻呋酰胺体积比5:5复配处理的毒性比率1.95，表现为增效；盆栽试验中，高浓度组合体积比5:5复配处理（终浓度为0.5×10⁸ CFU/mL YJ02+5 mg/L噻呋酰胺）的预防防效最优，较YJ02、噻呋酰胺单剂分别提高55.24%和46.04%，且预防处理防效均优于对应治疗处理；大田试验中，高浓度组合体积比5:5复配处理（终浓度为0.5×10⁸ CFU/mL YJ02+5 mg/L噻呋酰胺）防效与噻呋酰胺田间推荐用量相当，但可减少噻呋酰胺施药量。本研究为花生白绢病的绿色高效防控提供了具体技术方案与应用依据。

关键词: 贝莱斯芽孢杆菌YJ02, 花生白绢病, 齐整小核菌, 复配, 增效

Abstract: To improve the control efficiency of Bacillus velezensis YJ02 against peanut southern blight caused by Sclerotium rolfsii, this study explored the potential of integrating B. velezensis YJ02 with chemical fungicides for disease management. The compatibility of nine chemical fungicides commonly utilized for peanut southern blight control with B. velezensis YJ02 was evaluated using the dilution plating method and colony counting technique. The in vitro toxicity of compatible fungicides and B. velezensis YJ02 against S. rolfsii was determined via the toxic medium method, and the optimal mixing ratio of compatible fungicides to B. velezensis YJ02 was selected based on the toxicity ratio. Pot and field experiments were further conducted to verify the synergistic control effect of the screened composite formulation against peanut southern blight. The results demonstrated that three fungicides, namely thifluzamide, azoxystrobin, and trifloxystrobin, maintained excellent compatibility with B. velezensis YJ02, and thifluzamide exhibited the highest toxicity against S. rolfsii among them. The composite formulation consisting of B. velezensis YJ02 (1× 10² CFU/mL) and thifluzamide (0.1 mg/L) at a volume ratio of 5:5 showed a significant synergistic effect with a toxicity ratio of 1.95. In pot experiments, the high-concentration 5:5 composite formulation (the final concentration was 0.5× 10⁸ CFU/mL B. velezensis YJ02 and 5 mg/L thifluzamide) achieved the optimal preventive control efficacy, which was 55.24% and 46.04% higher than that of B. velezensis YJ02 and thifluzamide applied alone, respectively. Notably, the preventive efficacy of all treatments was significantly better than their corresponding curative efficacy. In field experiments, the control efficacy of the aforementioned high-concentration 5:5 composite formulation was comparable to that of thifluzamide applied at the recommended field dosage, while reducing the application amount of thifluzamide by more than 90%. This study provides a specific technical strategy and theoretical foundation for the green and efficient management of peanut southern blight, contributing to the sustainable control of this soil-borne disease in peanut production.

Key words: Bacillus velezensis YJ02, peanut southern blight, Sclerotium rolfsii, combanition, synergistic effect

中图分类号:

S476

范腕腕, 马毅辉, 刘杰, 郭雪萍, 杨玉媛. 贝莱斯芽孢杆菌YJ02复配噻呋酰胺对花生白绢病增效防治作用[J]. 中国生物防治学报, 2026, 42(1): 35-43.

FAN Wanwan, MA Yihui, LIU Jie, GUO Xueping, YANG Yuyuan. Synergistic Effect of Bacillus velezensis YJ02 Combined with Thifluzamide on Peanut Southern Blight[J]. Chinese Journal of Biological Control, 2026, 42(1): 35-43.

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