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中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (5): 1244-1252.DOI: 10.16409/j.cnki.2095-039x.2023.02.046

• 研究论文 • 上一篇    下一篇

木霉菌协同杀菌剂防治北苍术白绢病的增效作用

贺字典1, 韩亚梅1, 金歌1, 高玉峰2, 闵康1   

  1. 1. 河北科技师范学院农学与生物科技学院/河北省作物逆境生物学重点实验室, 秦皇岛 066600;
    2. 河北科技师范学院学报编辑部, 秦皇岛 066600
  • 收稿日期:2022-11-14 出版日期:2023-10-08 发布日期:2023-10-25
  • 通讯作者: 贺字典
  • 作者简介:贺字典,女,博士,教授,E-mail:zidianhe@163.com。
  • 基金资助:
    河北省重点研发项目(21326516D和22326501D);河北省自然科学基金项目(C2019407103);秦皇岛市重点研发项目(201901B022);河北省研究生创新资助项目(CXZZ202206)

Synergistic Effect of Trichoderma and Fungicides on Atractylodes chinensis Southern Blight Caused by Sclerotium rolfsii

HE Zidian1, HAN Yamei1, JIN Ge1, GAO Yufeng2, MIN Kang1   

  1. 1. Hebei Key Laboratory of Crop Stress Biology/College of Agronomy and Biotechnology, Hebei Normal University of Science & amp;Technology, Qinhuangdao 066000, China;
    2. Editorial Department of Journal, Qinhuangdao 066000, China
  • Received:2022-11-14 Online:2023-10-08 Published:2023-10-25

摘要: 为了提高木霉菌和杀菌剂协同对北苍术白绢病的防治效果,减少杀菌剂的使用量,本文采用生长速率法测定了12种杀菌剂对齐整小核菌及其拮抗木霉菌株的EC50,并采用平板对峙法和协同系数法筛选出与3种木霉菌具有生物相容性和增效作用的杀菌剂后,测定杀菌剂与木霉菌协同对北苍术白绢病的田间防治效果。结果表明:240 g/L噻呋酰胺SC、30%噁霉灵AS和80%代森锰锌WP与棘孢木霉T16、深绿木霉T15和哈茨木霉T11间的VEC50均大于1,具有良好的生物相容性。噻呋酰胺与棘孢木霉和深绿木霉协同对北苍术白绢病的田间防治效果分别达到了80.76%和78.82%,比噻呋酰胺的防治效果分别增长了13.36%和10.64%。由此可见,在减少化学杀菌剂使用量时,棘孢木霉T16和深绿木霉T15均可与噻呋酰胺协同防治北苍术白绢病且具有增效作用。

关键词: 北苍术白绢病, 棘孢木霉, 深绿木霉, 噻呋酰胺, 协同作用

Abstract: In order to control effectively southern blight caused by Sclerotium rolfsii of Atractylodes chinensis as well as reducing the dosage of chemical fungicides, Trichoderma strains that can be used in combination with fungicides were screened out. Toxicity of 12 chemical fungicides agents and 7 Trichoderma stains against S. rolfsii was firstly tested in vitro with toxic medium method. And Trichoderma asperellum T16, Trichoderma atroviride T15 and Trichoderma harzianum T11 were subsequently shown to exhibit bio-compatibility with fungicides proved by dural culture and synergistic effect method. Control efficacy of the combination on suppressing of southern blight of A.chinensis was further tested in field experiment. The results showed that the VEC50between240 g/L thifluzamide SC, 30% hymexazol AS and 80% mancozeb WP with T. asperellum T16, T. atroviride T15 and T. harzianum T11 were higher than 1, respectively, which indicated good bio-compatibility. In the reducing half dosage of thifluzamide condition, the synergistic control effect of thifluzamide combined with T. asperellum T16 and T. atroviride T15 on southern blight reached 80.76% and 78.82%, respectively, which increased by 13.36% and 10.64% compared with thifluzamide control. In conclusion, T. asperellum T16 and T. atroviride T15could synergize with thifluzamide to control A. chinensis southern blight, which was much more effective than applying 100% fungicides.

Key words: Atractylodes chinensis southern blight disease, Trichoderma asperellum, Trichoderma atroviride, thifluzamide, synergistic effect

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