木霉共培养发酵对黄瓜枯萎病的防治效果

doi:10.16409/j.cnki.2095-039x.2021.06.013

中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (1): 108-114.DOI: 10.16409/j.cnki.2095-039x.2021.06.013

木霉共培养发酵对黄瓜枯萎病的防治效果

陈凯¹, 隋丽娜², 赵忠娟¹, 李玲¹, 扈进冬¹, 李纪顺¹

1. 齐鲁工业大学(山东省科学院)/山东省科学院生态研究所, 济南 250103;
2. 齐鲁工业大学(山东省科学院)/生物工程学院, 济南 250353

收稿日期:2021-03-05 出版日期:2022-02-08 发布日期:2022-03-01
通讯作者: 李纪顺,应用研究员,E-mail:yewu2@sdas.org
作者简介:陈凯,高级工程师,E-mail:chenkai19960917@163.com
基金资助:
山东省重点研发计划项目（2019JZZY010718、2019JZZY010723）；山东省重点研发计划（2020CXGC010803、2020CXGC010805）；山东省自然基金博士基金（ZR2019BC076）；济南市泉城高端项目（2019T06）；山东省科学院院地产学研协同创新基金（2019-CXY5、2019-CXY21）；山东省科学院科教产融合创新试点工程（国际合作）项目（2020KJC-GH07）；山东省自然基金青年基金（ZR2020QC044）

Control Effect of Co-cultured Fermentation of Two Trichoderma Strains on Cucumber Fusarium Wilt

CHEN Kai¹, SUI Lina², ZHAO Zhongjuan¹, LI Ling¹, HU Jindong¹, LI Jishun¹

1. Ecology Institute, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250103, China;
2. School of Bioengineering, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250353, China

Received:2021-03-05 Online:2022-02-08 Published:2022-03-01

摘要/Abstract

摘要： 为明确深绿木霉HB20111和哈茨木霉TW21990共培养发酵对黄瓜枯萎病的防治作用，采用对峙培养法观察两菌株的亲和性，通过浸种抑菌萌发试验筛选共培养发酵的最佳条件，并在温室条件下验证其生防效果。结果表明：在PDA平板上25℃对峙培养5 d，两株木霉亲和性较好。两菌株等量接种发酵培养基，25℃、130 r/min振荡培养72 h，共培养发酵液500倍稀释浸种处理，对黄瓜种子根长的促生效果显著（P<0.05）。温室条件下，共培养发酵液500倍稀释液浸种对黄瓜枯萎病的温室防治效果和黄瓜幼苗增重率分别达73.65%和35.64%，较单菌株HB20111、TW21990分别提高了6.76%、9.46%和11.54%、13.31%；同时共培养发酵液也显著降低了黄瓜根际土尖孢镰孢拷贝数（P<0.05）。HB20111和TW21990共培养具有协同增效作用，本文为高效生物农药的研发提供了新思路。

关键词: 深绿木霉HB20111, 哈茨木霉TW21990, 共培养发酵, 黄瓜枯萎病, 防治效果

Abstract: To ascertain the potential use of co-cultured two Trichoderma strains (T. atroviride HB20111 and T. harzianum TW21990) to control Fusarium oxysporum f. sp. cucumerium Owen (FOC), the optimum condition for co-cultured fermentation were detected by plate confrontation culture and seeds germination test, and the control efficiency were detected by pot test in greenhouse. The results showed that HB20111 and TW21990 had good compatibility after co-cultivation on PDA plate at 25℃ for 5 days. A co-culture with the inoculation of the two organisms in the ratio of 1:1, shaked in 130 r/min for 72 h at 25℃, the co-cultured fermentation diluted 500 times could enhance root lengths of cucumber seeds (P<0.05) by soaking seeds treatment. Greenhouse test demonstrated that co-cultured fermentation diluted 500 times had the control efficacy of 73.65% and increased the seedling fresh weight at rate of 35.64% by soaking seeds treatment, respectively. Compared with singly-cultured strain HB20111 and TW21990, the efficiencies of the paired were significantly increased by 6.76%, 9.46% and 11.54%, 13.31%, respectively. Co-cultured fermentation treatment was also significantly reduced the FOC DNA copy numbers in cucumber rhizosphere soils (P<0.05). Therefore, co-cultured HB20111 and TW21990 had significant synergistic effect on the control of cucumber Fusarium wilt, which provided a new insight into development of highly efficient biocontrol agents.

Key words: Trichoderma atroviride HB20111, Trichoderma harzianum TW21990, co-cultured fermentation, cucumber Fusarium wilt, control efficiency

中图分类号:

S436.421.1

陈凯, 隋丽娜, 赵忠娟, 李玲, 扈进冬, 李纪顺. 木霉共培养发酵对黄瓜枯萎病的防治效果[J]. 中国生物防治学报, 2022, 38(1): 108-114.

CHEN Kai, SUI Lina, ZHAO Zhongjuan, LI Ling, HU Jindong, LI Jishun. Control Effect of Co-cultured Fermentation of Two Trichoderma Strains on Cucumber Fusarium Wilt[J]. Chinese Journal of Biological Control, 2022, 38(1): 108-114.

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木霉共培养发酵对黄瓜枯萎病的防治效果

Control Effect of Co-cultured Fermentation of Two Trichoderma Strains on Cucumber Fusarium Wilt

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