多粘类芽胞杆菌JE53对香梨采后黑斑病菌抑制效果及贮藏品质的影响

doi:10.16409/j.cnki.2095-039x.2023.02.048

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (5): 1266-1274.DOI: 10.16409/j.cnki.2095-039x.2023.02.048

• 研究论文 • 上一篇

多粘类芽胞杆菌JE53对香梨采后黑斑病菌抑制效果及贮藏品质的影响

郝金辉¹, 林胜楠^1,2, 吴梓菲^1,2, 王宁^1,2, 詹发强¹, 史应武¹, 何亚芳¹, 杨蓉¹, 侯新强¹, 包慧芳¹

1. 新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室, 乌鲁木齐 830091;
2. 新疆农业大学农学院, 乌鲁木齐 830052

收稿日期:2022-11-14 出版日期:2023-10-08 发布日期:2023-10-25
通讯作者: 包慧芳,研究员,E-mail:779556527@qq.com。
作者简介:郝金辉,女,硕士研究生,E-mail:1539578962@qq.com;林胜楠,女,硕士研究生,E-mail:1825879102@qq.com。
基金资助:
农业科技创新稳定支持项目（xjnkywdzc-2023005-6）；新疆自治区优秀青年科技人才培养项目（2020Q027）；新疆农业科学院科技创新重点培育专项（xjkcpy-2020006）

Effect of Paenibacillus polymyxa JE53 on Korla Pear Black Spot Pathogen and Fruit Quality during Storage

HAO Jinhui¹, LIN Shengnan^1,2, WU Zifei^1,2, WANG Ning^1,2, ZHAN Faqiang¹, SHI Yingwu¹, HE Yafang¹, YANG Rong¹, HOU Xinqiang¹, BAO Huifang¹

1. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences/Xinjiang Key Laboratory of Special Environmental Microbiology, Urumqi 830091, China;
2. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China

Received:2022-11-14 Online:2023-10-08 Published:2023-10-25

摘要/Abstract

摘要： 探究多粘类芽胞杆菌Paenibacillus polymyxa JE53对互隔交链孢Alternaria alternata的抑制效果及香梨贮藏品质的影响，为香梨采后黑斑病的生物防控奠定基础。以无菌水（CK）和1-甲基环丙烯（1-MCP）为对照组、JE53发酵液为处理组，分别处理香梨果实后，一部分采用损伤接种香梨黑斑病菌互隔交链孢A. alternata，置于人工气侯箱贮藏，测定JE53对链格孢菌的抑制效果；一部分置于人工气侯箱贮藏，定期测定果实贮藏品质和生理特性指标。结果表明：JE53可有效抑制梨黑斑病菌在梨果实上的生长，损伤接种条件下，贮藏144 h时抑菌率为30.73%；还可显著提高梨果实中过氧化物酶、过氧化氢酶、苯丙氨酸解氨酶活性，能较好保持果实色度、硬度及可溶性固形物和可滴定酸含量，减少丙二醛积累及抗坏血酸损耗，降低果实失重率。上述结果表明：JE53可有效抑制香梨采后黑斑病病原菌在果实上的生长，并能较好的保持果实品质，可作为香梨采后黑斑病生防菌资源。

关键词: 多粘类芽胞杆菌, 生物防治, 贮藏品质, 生理特性

Abstract: In order to lay a foundation for the biological control of postharvest black spot of fragrant pear, the inhibition effect of Bacillus polymyxa JE53 on Alternaria alternata and the storage quality of fragrant pear were studied. Fragrant pear fruits were treated with sterile water (CK) and 1-methylcyclopropene (1-MCP) respectively as control group and treated with JE53 fermentation liquid as treatment group. Some of them were inoculated with A. alternata after being punctured, and stored in an artificial weather box to determine the inhibitory effect of JE53 on A. alternata. The others were stored in an artificial weather box to determination of fruit storage quality and physiological characteristics. The results showed that JE53 can effectively inhibit the growth of pear black spot fungus on pear fruit, and the antibacterial rate was 30.73% after inoculation and storage for 144 hours. It could also significantly increase peroxidase, catalase, and phenylalanine in pear fruit. In addition, the peel color, hardness, soluble solid and titrable acid content of pear fruits of the treatment group were better than those of the control group. The accumulation of malondialdehyde and the loss of ascorbic acid as well as the fruit weight loss rate was reduced. In conclusion: JE53 can effectively inhibit the growth of the pathogenic bacteria of black spot disease in fragrant pears after harvest, and can maintain the fruit quality better, it can be used as a resource of biocontrol bacteria for postharvest black spot disease in fragrant pears.

Key words: Paenibacillus polymyxa, biological control, storage quality, physiological property

中图分类号:

S476

郝金辉, 林胜楠, 吴梓菲, 王宁, 詹发强, 史应武, 何亚芳, 杨蓉, 侯新强, 包慧芳. 多粘类芽胞杆菌JE53对香梨采后黑斑病菌抑制效果及贮藏品质的影响[J]. 中国生物防治学报, 2023, 39(5): 1266-1274.

HAO Jinhui, LIN Shengnan, WU Zifei, WANG Ning, ZHAN Faqiang, SHI Yingwu, HE Yafang, YANG Rong, HOU Xinqiang, BAO Huifang. Effect of Paenibacillus polymyxa JE53 on Korla Pear Black Spot Pathogen and Fruit Quality during Storage[J]. Chinese Journal of Biological Control, 2023, 39(5): 1266-1274.

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多粘类芽胞杆菌JE53对香梨采后黑斑病菌抑制效果及贮藏品质的影响

Effect of Paenibacillus polymyxa JE53 on Korla Pear Black Spot Pathogen and Fruit Quality during Storage

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