淡紫拟青霉·哈茨木霉微胶囊剂的制备及对苹果轮纹病菌的抑制作用

doi:10.16409/j.cnki.2095-039x.2024.02.009

摘要/Abstract

摘要： 为延长微生物菌剂货架期，提高其在环境中存活率，保证其药效，本研究采用复凝聚法分别制备淡紫拟青霉和哈茨木霉微胶囊剂。微胶囊剂以哈茨木霉菌和淡紫拟青霉菌为芯材，明胶和阿拉伯胶为壁材，通过响应面法确定了微胶囊的最佳包埋条件。淡紫拟青霉微胶囊成囊条件为：明胶和阿拉伯胶各2.5%（ 40 mL）为壁材溶液、孢子浓度1× 10⁹cfu/mL、固化pH和温度分别为： 4℃和40℃、搅拌速度500 r/min，最终包埋率达（ 85.44± 0.22）%，中位粒径为23.62 μm。4℃下贮存720 d，孢子存活率为95.23%；哈茨木霉成囊条件为：明胶和阿拉伯胶各1.6%（ 40 mL）为壁材溶液、孢子浓度1× 10⁹cfu/mL、固化pH和温度分别为： 4.2℃和40℃、搅拌速度500 r/min，包埋率达92.17%，中位粒径为27.19 μm。25℃下的存活率为92.11%。4℃下贮存720 d后，孢子存活率为96.25%。将制备的复合微胶囊剂用于苹果轮纹病的室内离体测试，当哈茨木霉微胶囊与淡紫拟青霉微胶囊以浓度6:4的比例进行混合后，抑制效果可达60.0%。

关键词: 微胶囊, 淡紫拟青霉, 哈茨木霉, 苹果轮纹病

Abstract: To prolong shelf life of the microbial formulation and improve its survival rate in the environment to ensure its efficacy,a complex coacervation was used to prepare Paecilomyces lilacinus and Trichoderma harzianum microcapsule formulation separately.In this study,taking conidial spores of T.harzianum and P.lilacinu as the core materials,gelatin and arabic gum as the wall materials,the optimal investment conditions of microcapsule were determined by response surface method.The encapsulation conditions for P.lilacinus microcapsules were 2.5% of gelatin and arabic gum (40 mL),respectively,as the wall materials solution,1× 10⁹cfu/mL spores,curing pH 4,curing temperature of 40℃,stirring speed of 500 r/min,the final encapsulated rate of (85.44± 0.22)%,and the median size of the encapsulated particles of 23.62 μm.The spore survival rate was 95.23% after 720 days of storage at 4℃.The encapsulation conditions for T.harzianum microcapsules were 1.6% of gelatin and arabic gum (40 mL),respectively,as the wall materials,1× 10⁹cfu/mL spores,curing pH 4.2,curing temperature of 40℃,stirring speed of 500 r/min,the final encapsulated rate of 92.17%,and the median size of the encapsulated particles of 27.19 μm.The survival rate at 25℃ was 92.11%.After 720 days storage at 4℃,the survival rate of spores was 96.25%.A combined formulation with T.harzianum and P.lilacinus microcapsules at the ratio of 6:4 was applied to an indoor in-vitro test to inhibit Physalospora piricola Nose.The results showed that the inhibition activity of the formulation reached 60.0%.

Key words: microcapsule, Paecilomyces lilacinus, Trichoderma harzianum, Physalospora piricola Nose

中图分类号:

S476

王凯, 张开娇, 李洋洋, 杨大杰, 殷志鹏, 何璐. 淡紫拟青霉·哈茨木霉微胶囊剂的制备及对苹果轮纹病菌的抑制作用[J]. 中国生物防治学报, 2024, 40(2): 388-398.

WANG Kai, ZHANG Kaijiao, LI Yangyang, YANG Dajie, YIN Zhipeng, HE Lu. Microcapsule Preparation of Penicillium lilac and Trichoderma harzia,and It Inhibition Activity against Physalospora piricola Nose[J]. Chinese Journal of Biological Control, 2024, 40(2): 388-398.

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