拮抗放线菌2-1-2F的鉴定及其对黄瓜枯萎病的防效

doi:10.16409/j.cnki.2095-039x.2024.02.006

摘要/Abstract

摘要： 为获得可高效防控黄瓜枯萎病的生防放线菌，采用梯度稀释和平板对峙法从蔬菜根际土壤中筛选到1株对黄瓜枯萎病菌Fusarium oxysporum f.sp.cucumerinm（ FOC）有较强拮抗作用的放线菌2-1-2F；经形态学和分子生物学鉴定，确定菌株2-1-2F为弗吉尼亚链霉菌Streptomyces virginiae。其能产生蛋白酶、β-葡聚糖酶和几丁质酶，不产生嗜铁素和纤维素酶，且菌株2-1-2F产生的发酵滤液活性易受环境条件影响。菌株2-1-2F发酵菌液对黄瓜有明显的促生作用，可诱导黄瓜体内防御酶活性和抗性相关物质含量增加，并于处理后1～7 d内达最大值。菌株2-1-2F在黄瓜根部有较强的定殖能力，42 d后在土壤中仍可达10⁶ cfu/g；其对黄瓜枯萎病的预防和治疗效果分别达48.98%和34.01%。

关键词: 弗吉尼亚链霉菌, 鉴定, 黄瓜枯萎病, 生防效果

Abstract: In order to obtain biocontrol actinomycetes to control cucumber Fusarium wilt,a strain 2-1-2F with strong antagonistic activity against F.oxysporum f.sp.cucumerinum(FOC) was isolated from vegetable rhizospheric soil by gradient dilution and confrontation culture methods.Strain 2-1-2F was identified as Streptomyces virginiae by morphology observation and molecular biology identification.It could produce proteases,chitinases and β-glucanases,but not produce siderophores and cellulases.The activity of fermentation liquid produced by 2-1-2F was susceptible to environmental conditions.Fermentation liquid of 2-1-2F had obvious growth-promoting effect on cucumber plants,and could induce the cucumber defense enzyme activities and resistance related substance contents,which reached the maximum value from one to seven days after treatment.Strain 2-1-2F had strong colonization ability in cucumber roots,and the concentration of colonization could still reach 10⁶ cfu/g soil after 42 days.The preventive and therapeutic effects of 2-1-2F on cucumber Fusarium wilt were 48.98% and 34.01%,respectively.Actinomycetes 2-1-2F and its metabolites had the potential to be developed into biocontrol agents.

Key words: Streptomyces virginiae, identification, cucumber Fusarium wilt, control effect

中图分类号:

S476

蒋冬阳, 吴冰越, 季文平, 陈宸, 陈夕军. 拮抗放线菌2-1-2F的鉴定及其对黄瓜枯萎病的防效[J]. 中国生物防治学报, 2024, 40(2): 411-423.

JIANG Dongyang, WU Bingyue, JI Wenping, CHEN Chen, CHEN Xijun. Identification of Antagonistic Actinomycetes 2-1-2F and Its Control Effect on Cucumber Fusarium Wilt[J]. Chinese Journal of Biological Control, 2024, 40(2): 411-423.

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