溜曲霉Aspergillus tamarii XJ-2与6种杀虫剂相容性及对亚洲玉米螟田间联合防效评价

doi:10.16409/j.cnki.2095-039x.2026.01.038

摘要/Abstract

摘要： 采用室内毒力测定法明确了杀虫剂四唑虫酰胺，阿维菌素，乙基多杀菌素，高效氯氟氰菊酯，氯虫苯甲酰胺，溴氰虫酰胺对亚洲玉米螟Ostrinia furnacalis 3龄幼虫的室内毒力。结果表明，5%阿维菌素对亚洲玉米螟3龄幼虫毒力最高，LC₅₀为0.325 mg/L;6%乙基多杀菌素和20%四唑虫酰胺次之，LC₅₀分别为1.129 mg/L和4.547 mg/L;20%高效氯氟氰菊酯最低，LC₅₀为47.342 mg/L。进一步筛选上述杀虫剂与溜曲霉XJ-2的相容性，发现四唑虫酰胺与溜曲霉XJ-2相容性最好，0.3 mg/L时对菌株XJ-2生长速率、产孢量和孢子萌发的抑制率分别为16.47%、31.54%和3.27%；高效氯氟氰菊酯次之，其抑制率分别为28.37%、39.96%和17.36%；阿维菌素和氯虫苯甲酰胺相容性最差，其生长速率、产孢量和孢子萌发率的抑制率分别为43.26%、52.25%、63.86%和40.76%、49.52%、65.32%。其生长速率、产孢量和孢子萌发率的抑制率分别为43.26%、52.25%、63.86%和40.76%、49.52%、65.32%。室内复配毒力测定表明，四唑虫酰胺与溜曲霉XJ-2过不同体积配比（10:0、9:1、4:1、1:1、1:4、1:9和0:10）处理均表现出显著的协同增效作用（毒性比率>1），其中4:1配比的共毒系数高达339（毒性比率为1.07），综合效果最优。单剂对照中，四唑虫酰胺的室内死亡率（79.74%）显著高于XJ-2单剂（50.68%）。田间药效试验显示，四唑虫酰胺与溜曲霉XJ-2复配在药后21 d防效达到最高，校正防效为87.20%，均显著高于单独使用四唑虫酰胺（79.74%）和溜曲霉XJ-2孢子悬浮液（50.68%），也优于金龟子绿僵菌CQMa421悬浮剂（51.80%）及球孢白僵菌ZJU435悬浮剂（65.22%）。此外，20%四唑虫酰胺与溜曲霉XJ-2联用可显著延长田间持效期，其在49 d时的防效较单独使用四唑虫酰胺和溜曲霉XJ-2孢子悬浮液分别高10.24%、23.82%，可作为亚洲玉米螟绿色防控的高效策略。

关键词: 溜曲霉, 相容性, 共毒系数, 田间防效

Abstract: Insecticidal activities of tetraniliprole, abamectin, spinetoram, lambda-cyhalothrin, chlorantraniliprole, and cyantraniliprole against Ostrinia furnacalis were evaluated by laboratory assay. The results showed that 5% abamectin had the highest toxicity to the 3 rd instar larvae of O. furnacalis, with an LC₅₀ of 0.325 mg/L, followed by 6% spinetoram and 20% tetraniliprole, with LC₅₀ values of 1.129 mg/L and 4.547 mg/L, respectively, while 20% lambda-cyhalothrin had the lowest toxicity, with an LC₅₀ of 47.342 mg/L. The compatibility of the above insecticides with Aspergillus tamari XJ-2 was determined, with tetraniliprole showing the best compatibility with A. tamari XJ-2. At 0.3 mg/L, the inhibition rates on the growth rate, sporulation, and spore germination of A. tamari XJ-2 were 16.47%, 31.54%, and 3.27%, respectively, followed by lambda-cyhalothrin, with inhibition rates of 28.37%, 39.96%, and 17.36%, respectively. Abamectin and chlorantraniliprole showed the poorest compatibility, with inhibition rates on growth rate, sporulation, and spore germination of 43.26%, 52.25%, and 63.86%, and 40.76%, 49.52%, and 65.32%, respectively. Laboratory mixture toxicity tests showed that tetraniliprole combined with A. tamari XJ-2 at different volume ratios（10:0, 9:1, 4:1, 1:1, 1:4, 1:9, and 0:10） all exhibited significant synergistic effects（toxicity ratio > 1）. Among them, the 4:1 ratio achieved the optimal comprehensive effect, with a co-toxicity coefficient as high as 339 and a toxicity ratio of 1.07. In the single-agent controls, the mortality rate of tetraniliprole（79.74%） was significantly higher than that of the XJ-2 single agent（50.68%）. Field trials showed that the combination of tetraniliprole and A. tamari XJ-2 reached the highest efficiency at 21 days post-treatment, with a corrected control efficiency of 87.20%, which was significantly higher than those of tetraniliprole alone（79.74%） and A. tamari XJ-2 spore suspension alone（50.68%）, and also superior to Metarhizium anisopliae CQMa421 suspension（51.80%） and Beauveria bassiana ZJU435 suspension（65.22%）. Furthermore, the combination of 20% tetraniliprole and A. tamari XJ-2 significantly prolonged the duration of field efficacy. Compared to tetraniliprole alone and A. tamari XJ-2 spore suspension alone, its efficiency at 49 days post-treatment was 10.24% and 23.82% higher, respectively, indicating that this combination can serve as an efficient strategy for the green control of O. furnacalis.

Key words: Aspergillus tamarii, compatibility, co-toxicity coefficient, field control efficacy

中图分类号:

S451
S476.1

董玉琦, 王小武, 马雪茹, 程苗, 胡泽源, 张斐然, 付开赟, 吐尔逊·阿合买提, 贾尊尊, 丁新华, 郭文超. 溜曲霉Aspergillus tamarii XJ-2与6种杀虫剂相容性及对亚洲玉米螟田间联合防效评价[J]. 中国生物防治学报, 2026, 42(4): 828-837.

DONG Yuqi, WANG Xiaowu, MA Xueru, CHENG Miao, HU Zeyuan, ZHANG Feiran, FU Kaiyun, TURSUN Ahmat, JIA Zunzun, DING Xinhua, GUO Wenchao. Compatibility of Aspergillus tamarii XJ-2 with Six Insecticides and Their Combined Field Control Efficiency against Ostrinia furnacalis[J]. Chinese Journal of Biological Control, 2026, 42(4): 828-837.

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