苏云金芽胞杆菌G033A与不同药剂联合施用对苹果蠹蛾的防治效果

doi:10.16409/j.cnki.2095-039x.2025.05.004

摘要/Abstract

摘要： 苹果蠹蛾Cydia pomonella以幼虫钻蛀果实为害，严重威胁我国新疆地区苹果、梨、核桃等产业的可持续发展。本研究采用直接喷雾法测定了苏云金芽胞杆菌G033A（Bt G033A）以及甲氨基阿维菌素苯甲酸盐、苦参碱、球孢白僵菌对苹果蠹蛾1龄幼虫的室内毒力，并评估了Bt G033A分别与其余3种药剂的联合毒力和田间防效。在室内条件下，Bt G033A、甲氨基阿维菌素苯甲酸盐、苦参碱、球孢白僵菌对苹果蠹蛾1龄幼虫的LC50分别为1.17×10⁶ IU/L、0.02 mg/L、38.35 mg/L和6.25×10⁹孢子/L。致死中浓度的BtG033A与球孢白僵菌以体积比1:1混配后增效作用最显著，协同毒力指数为100.02。田间200 g/667 m²剂量的Bt G033A单次施用7 d后对苹果蠹蛾防效为53.79%～64.02%，100 g/667 m²球孢白僵菌与100 g/667 m²Bt G033A复配，防治效果达89.98%～92.24%，高于同等剂量球孢白僵菌和Bt G033A单剂的防效。这表明，Bt G033A与球孢白僵菌联合使用显著提高了对苹果蠹蛾的杀虫活性和田间防效。

关键词: 苏云金芽胞杆菌, 球孢白僵菌, 杀虫活性, 农药复配, 田间防效

Abstract: The codling moth, Cydia pomonella, a destructive pest whose larvae bore into fruits, poses a severe threat to the sustainable development of apple, pear, and walnut industries in Xinjiang, China. This study evaluated the toxicity of Bacillus thuringiensis G033A (Bt G033A), emamectin benzoate, matrine, and Beauveria bassiana against first-instar larvae of C. pomonella using direct spray assays under laboratory conditions. Furthermore, the combined toxicity and field control efficiency of Bt G033A paired with the other three agents were assessed. Laboratory bioassays revealed LC₅₀ values are 1.17×10⁶ IU/L (Bt G033A), 0.02 mg/L (emamectin benzoate), 38.35 mg/L (matrine), and 6.25×10⁹ spores/L (B. bassiana), respectively. The most significant synergism was observed in the 1:1 LC₅₀ combination of Bt G033A and B. bassiana, with a synergistic toxicity index (CF) of 100.02. Field trials (spray volume: 40 L/667 m²) showed that single application of 200 g/667 m² Bt G033A achieved control of 53.79%—64.02% at 7 days post treatment. The combination B. bassiana (100 g/667 m²) with Bt G033A (100 g/667 m²) improved control to 89.98%—92.24%. These findings indicate that the combined application of Bt G033A and B. bassiana significantly enhances insecticidal activity and field control of C. pomonella.

Key words: Bacillus thuringiensis G033A, Beauveria bassiana, insecticidal activity, pesticide combination, field control efficacy

中图分类号:

S476.1

马兴莹, 杨龙, 王凯涛, 姜岩, 吴少英, 陆宴辉. 苏云金芽胞杆菌G033A与不同药剂联合施用对苹果蠹蛾的防治效果[J]. 中国生物防治学报, 2026, 42(2): 396-402.

MA Xingying, YANG Long, WANG Kaitao, JIANG Yan, WU Shaoying, LU Yanhui. Synergistic Control Efficiency of Bacillus thuringiensis G033A Combined with Multiple Insecticides Against Codling Moth (Cydia pomonella)[J]. Chinese Journal of Biological Control, 2026, 42(2): 396-402.

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