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中国生物防治学报 ›› 2026, Vol. 42 ›› Issue (4): 875-885.DOI: 10.16409/j.cnki.2095-039x.2025.11.015

• 研究论文 • 上一篇    

生防菌合成菌群泡腾颗粒的研制及其对辣椒疫病的防控效果

鲁凯珩1, 周佳慧1, 李秋玲1, 张雷刚2, 余向阳2, 刘常宏1   

  1. 1. 南京大学生命科学学院/医药生物全国重点实验室, 南京 210023;
    2. 江苏省农业科学院食品质量安全与营养研究所/江苏省食品质量安全重点实验室, 南京 210014
  • 收稿日期:2025-08-11 发布日期:2026-08-26
  • 通讯作者: 刘常宏,博士研究生,教授,E-mail:chliu@nju.edu.cn。
  • 作者简介:鲁凯珩,博士研究生,E-mail:lukaiheng@outlook.com;周佳慧,硕士研究生,E-mail:zjh2363281523@163.com。
  • 基金资助:
    江苏省农业科技自主创新资金项目(CX (22)2021);江苏省科技创新专项项目(BK20220036)

Development of Biocontrol Bacterial Synthetic Community Effervescent Granules and Their Control of Pepper Blight

LU Kaiheng1, ZHOU Jiahui1, LI Qiuling1, ZHANG Leigang2, YU Xiangyang2, LIU Changhong1   

  1. 1. State Key Laboratory of Pharmaceutical Biotechnology/School of Life Sciences, Nanjing University, Nanjing 210023, China;
    2. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2025-08-11 Published:2026-08-26

摘要: 本研究旨在开发一种基于6种生防菌构建的合成菌群(SynCom)及其泡腾颗粒(EG)剂型,用于辣椒疫病的绿色防控。采用单因素实验确定膨润土为最佳载体,结合Box-Behnken响应面法优化制备工艺,获得了制备SynCom EG的最佳参数:15 g膨润土吸附(37℃、236 r/min、153 min),24℃烘干。所得颗粒的崩解时间为52 s、活菌数达到2×107 CFU/g,室温储存6个月后存活率为63.5%。盆栽试验表明,该颗粒可显著促进辣椒生长,并对辣椒疫病表现出73.7%的防控效果。此研究为绿色农业提供了一种创新的生防技术。

关键词: 辣椒疫病, 合成菌群, 泡腾颗粒, 吸附优化, 生物防治

Abstract: This study aims to develop a synthetic microbial community(SynCom) based on six biocontrol bacteria and its effervescent granule(EG) formulation for the green control of pepper blight. A single-factor experiment identified bentonite as the optimal carrier. Subsequently, the Box-Behnken response surface method was used to optimize the preparation process, resulting in the best parameters for producing SynCom EG: 15 g of bentonite adsorbed at 37 ℃, with agitation at 236 rpm for 153 min, followed by drying at 24 ℃. The resulting granules had a disintegration time of 52 s and an active bacterial count of 2×107 CFU/g. After six months of storage at room temperature, the survival rate was 63.5%. Pot experiments demonstrated that these granules significantly promoted pepper growth and exhibited a 73.7% control efficiency against pepper blight. These findings provide an innovative biocontrol technology for sustainable pepper production.

Key words: Phytophthora blight, synthetic microbial communities, effervescent granules, adsorption optimization, biological control

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