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菜蛾盘绒茧蜂滞育与非滞育预蛹过氧化氢、抗氧化酶及激素水平的比较

郝仲萍, 袁忠勤, 张娟, 赵金瑞, 施祖华   

  1. 浙江大学昆虫科学研究所,杭州 310029
  • 收稿日期:2011-09-02 修回日期:1900-01-01 出版日期:2012-05-08 发布日期:2012-05-08
  • 通讯作者: 施祖华

Comparison of Hydrogen Peroxide, Antioxidant Enzymes and Hormones between Diapause and Non-diapause Prepupae of Cotesia vestalis

HAO Zhongping, YUAN Zhongqin, ZHANG Juan, ZHAO Jinrui, SHI Zuhua   

  1. Institute of Insect Sciences, Zhejiang University, Zhejiang Province, Hangzhou 310029, China
  • Received:2011-09-02 Revised:1900-01-01 Online:2012-05-08 Published:2012-05-08

摘要: 为了研究过氧化氢与激素在菜蛾盘绒茧蜂Cotesia vestalis滞育过程中的作用,本文测定了滞育与非滞育预蛹过氧化氢的含量,3种保护酶(SOD、POD和CAT)的活性以及20E和JH Ⅲ的滴度,结果显示,滞育预蛹过氧化氢含量、SOD和POD活性以及20E滴度显著低于非滞育预蛹,而CAT活性与JH Ⅲ滴度则相反。推测高浓度的JH Ⅲ抑制20E的合成和释放,20E滴度的下降又通过调节SOD活性的下降和CAT活性的上升来降低过氧化氢的浓度,使菜蛾盘绒茧蜂滞育。

Abstract: In order to investigate the effects of hydrogen peroxide and hormones on diapause in Cotesia vestalis, hydrogen peroxide content, activities of three antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), and contents of 20E and JH Ⅲ between diapause and non-diapause prepupae were compared. Results showed that the hydrogen peroxide content, SOD and POD activities and 20E content in diapause prepupae were significantly lower, while the CAT activity and JH Ⅲ content were significantly higher, when compared to those in non-diapause prepupae. We speculated that high concentrations of JH Ⅲ inhibited the synthesis and release of 20E, the latter reduced hydrogen peroxide content by decreasing SOD activity and increasing CAT activity, and finally caused prepupae of C. vestalis to enter diapause.

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