枯草芽孢杆菌PTS-394诱导番茄对灰霉病的系统抗性

doi:10.16409/j.cnki.2095-039x.2017.02.012

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (2/3): 219-225.DOI: 10.16409/j.cnki.2095-039x.2017.02.012

枯草芽孢杆菌PTS-394诱导番茄对灰霉病的系统抗性

乔俊卿¹, 张心宁², 梁雪杰¹, 刘永锋¹, 刘邮洲¹

1. 江苏省农业科学院植物保护研究所, 南京 210014;
2. 徐州市睢宁县农业委员会, 睢宁 221225

收稿日期:2016-07-05 出版日期:2017-06-08 发布日期:2017-04-08
通讯作者: 刘邮洲,博士,研究员,E-mail:shitouren88888@163.com。
作者简介:乔俊卿,博士,副研究员,E-mail:junqingqiao@hotmail.com;张心宁,高级农艺师,E-mail:snnyzxn@126.com
基金资助:
国家自然科学基金（31201556）；江苏省农业自主创新基金（CX-16-1021）；江苏省三新工程项目（SXGC（2016）057）

Plant System Resistance Triggered by Root-colonizing Bacillus subtilis PTS-394 and Its Control Effect on Tomato Gray Mold

QIAO Junqing¹, ZHANG Xinning², LIANG Xuejie¹, LIU Yongfeng¹, LIU Youzhou¹

1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. Agriculture Committee of Suining County, Suining 221225, China

Received:2016-07-05 Online:2017-06-08 Published:2017-04-08

摘要/Abstract

摘要： 本文研究了枯草芽孢杆菌PTS-394对番茄的防御相关酶活性、抗病信号转导通路的标志基因表达的诱导情况和诱导抗病性对灰霉病的防治效果。结果显示，菌株PTS-394灌根番茄后，在24~72 h内番茄顶端叶片中PAL、PPO、POX、LOX的活性都有不同程度的持续增加，且72 h时达到最高峰值，随后在96 h下降，与对照相比差异显著；此外，番茄抗病信号通路节点基因NPR1和水杨酸（SA）信号通路激发的防卫基因PR-1a，在24~72 h得到了显著持续高表达。以上结果表明，利用菌株PTS-394灌根番茄后，能够诱导植株产生系统抗病性。菌株PTS-394灌根番茄后48 h，离体叶片挑战接种番茄灰霉菌，结果显示，菌株PTS-394处理的番茄叶片病斑面积仅为对照处理的50%，防控效果达47.1%；温室盆栽试验显示，菌株PTS-394处理后对番茄灰霉的防治效果为58.2%。综上所述，枯草芽孢杆菌PTS-394灌根番茄后，可以触发番茄植株系统性的抗病性，增强植株免疫能力。

关键词: 枯草芽孢杆菌, 番茄, 防御酶, 信号转导通路

Abstract: The majority of plant growth promoting rhizobacteria (PGPR) has ability to reduce a plant's susceptibility to pathogen attack by activating plant defenses. In this study, the defense-related enzyme activity, signal transduction pathways and control efficiency against tomato gray mold were investigated to clarify the mechanism of tomato systemic resistance triggered by Bacillus subtilis PTS-394. After root drenching with PTS-394, the PAL, PPO, POX, LOX activity of the top tomato leaves increased at 24-72 h and peaked at 72 h, then declined at 96 h. Meanwhile, the key gene NPR1 in tomato resistance signaling pathway and defense gene PR-1a in salicylic acid (SA) signaling pathway were expressed significantly at 24-72 h after treatment with PTS-394. Accordingly, PTS-394 is capable of inducing systemic resistance in tomato plants. Infection experiment of Botrytis cinerea on detached tomato leaves indicated that the lesion area treated with PTS-394 was only half of that in the blank control, and the control efficacy against tomato grey mold was about 47.1%. Pot experiments in greenhouse showed that the control efficacy on tomato gray mold was 58.2%. In summary, the application of Bacillus subtilis PTS-394 could trigger tomato systemic disease resistance which plays an important role in the control of tomato gray mold.

Key words: Bacillus subtilis, tomato, defense-related enzyme, signal transduction pathways

中图分类号:

S476

乔俊卿, 张心宁, 梁雪杰, 刘永锋, 刘邮洲. 枯草芽孢杆菌PTS-394诱导番茄对灰霉病的系统抗性[J]. 中国生物防治学报, 2017, 33(2/3): 219-225.

QIAO Junqing, ZHANG Xinning, LIANG Xuejie, LIU Yongfeng, LIU Youzhou. Plant System Resistance Triggered by Root-colonizing Bacillus subtilis PTS-394 and Its Control Effect on Tomato Gray Mold[J]. journal1, 2017, 33(2/3): 219-225.

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枯草芽孢杆菌PTS-394诱导番茄对灰霉病的系统抗性

Plant System Resistance Triggered by Root-colonizing Bacillus subtilis PTS-394 and Its Control Effect on Tomato Gray Mold

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