深色有隔内生真菌甘瓶霉对番茄抗枯萎病的作用

doi:10.16409/j.cnki.2095-039x.2017.03.014

摘要/Abstract

摘要： 本文以深色有隔内生真菌（dark septate endophyte，DSE）甘瓶霉Phialophora mustea不同来源的2个菌株K36和Z48为生防菌株，对比研究DSE接种对番茄尖孢镰刀菌Fusarium oxysporum的抗性及其抗氧化酶的影响。研究表明，在尖孢镰刀菌胁迫下，与不接种DSE的对照相比，DSE接种可以显著缓解尖孢镰刀菌抑制番茄生长的症状，接种菌株K36和Z48后，植物株高分别增长了46.4%、53.2%，地上、地下部分干重分别增长了60.6%、50%和63.7%、65.9%，明显促进番茄生长和生物量的增加。进一步研究发现DSE接种显著激活番茄叶片SOD、POD等抗氧化酶活性进而降低尖孢镰刀菌导致的细胞脂质过氧化胁迫，发现在尖孢镰刀菌胁迫下，与不接种DSE的对照相比，接种菌株K36和Z48后番茄叶片SOD的酶活性分别增加19.3%和8.1%，POD的酶活性分别增加14.0%和4.4%，而MDA含量则显著减少了22.3%和9.1%。DSE接种增强了植物抗氧化酶活性，缓解由尖孢镰刀菌导致的脂膜过氧化胁迫，从而提高植物对真菌病害的抗性，促进宿主植物生长。

关键词: 深色有隔内生真菌, 甘瓶霉, 番茄, 抗病性, 尖孢镰刀菌, 抗氧化酶

Abstract: Tolerance of tomato plants (Lycopersicon esculentum) against fungal pathogen Fusarium oxysporum was investigated by inoculation with two biocontrol agents, dark septate endophyte (DSE) strains K36 and Z48 of Phialophora mustea, and the influence on antioxidant enzymes was also assayed. Under the treatment of F. oxysporum on tomato plants, the inoculation of two DSE strains significantly alleviated the growth inhibition of tomatoes, compared with the uninoculated-DSE controls. Inoculation of the strains K36 and Z48 significantly increased plant height by 46.4% and 53.2%, and enhanced tomato shoot and root biomasses by 60.6% and 50%, 63.7% and 65.9%, respectively. Moreover, DSE inoculation further triggered the antioxidant response of tomatoes, and increased the activities of SOD by 19.3% and 8.1%, POD by 14.0% and 4.4%, respectively. The enhanced antioxidant enzymes resulted in the alleviated cell-induced lipid peroxidation stress caused by fungal pathogens, compared with the uninoculated DSE treatments under F. oxysporum inoculation. Our results revealed that DSE colonization could improve the plant resistance against wilt disease caused by F. oxysporum and further promote the growth of host plants.

Key words: dark septate endophytes, Phialophora mustea, Lycopersicon esculentum, disease resistance, Fusarium oxysporum, antioxidant enzymes

中图分类号:

S476

张晓蓉, 李涛, 王超君, 朱玲玲, 徐露娟, 赵之伟. 深色有隔内生真菌甘瓶霉对番茄抗枯萎病的作用[J]. 中国生物防治学报, 2017, 33(2/3): 394-400.

ZHANG Xiaorong, LI Tao, WANG Chaojun, ZHU Lingling, XU Lujuan, ZHAO Zhiwei. Enhanced Tolerance of Tomatoes against Fusarium oxysporum by Inoculation with Dark Septate Endophyte[J]. journal1, 2017, 33(2/3): 394-400.

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