两种杀虫剂复配的固体纳米分散体的增效研究

doi:10.16409/j.cnki.2095-039x.2017.06.007

摘要/Abstract

摘要： 为延缓害虫对生物农药的抗性，显著提高生物农药有效利用率，本研究在排除剂型组成及制备工艺对杀虫活性影响的条件下，采用自乳化-载体固化法制备了具有生物农药特性的甲氨基阿维菌素苯甲酸盐和仿生型农药高效氯氟氰菊酯的复配固体纳米分散体。纳米制剂呈现出了良好的叶面浸润与滞留特性。高效氯氟氰菊酯和甲氨基阿维菌素苯甲酸盐质量比为3：1、2：1、1：1、1：2和1：3的复配纳米制剂处理棉蚜24 h后的LC₅₀分别为24.0、21.71、33.26、17.96和16.09 μg/mL，共毒系数分别为264.74、229.59、106.82、158.72和162.78，显著增效配比为3：1和2：1的组合。研究结果表明滞留量为影响制剂生物活性的关键指标。该杀虫剂复配纳米剂型的创制及增效效应的研究为延长生物农药使用寿命，解决害虫抗性问题提供了新的理论依据与技术途径，具有广阔的应用前景。

关键词: 甲氨基阿维菌素苯甲酸盐, 高效氯氟氰菊酯, 复配固体纳米分散体, 增效

Abstract: In order to retard insect resistance and significantly improve efficacy of biopesticides, the mixing solid nanodispersions of emamectin benzoate with biopesticide characteristics and bionic pesticide lambda-cyhalothrin were prepared by self-emulsifying and solidification method in the conditions of excluding the effects of formulation composition and preparation process. The nanoformulations exhibited excellent wettability and retention characteristics on leaves. The LC₅₀ of the mixing solid nanodispersions with 3:1, 2:1, 1:1, 1:2 and 1:3 (w/w) ratios of lambda-cyhalothrin to emamectin benzoate was 24.0, 21.71, 33.26, 17.96 and 16.09 μg/mL, respectively, when treated against cotton aphid for 24 h. The co-toxicity coefficients were 264.74, 229.59, 106.82, 158.72 and 162.78, respectively. The ratios with significant synergistic effect were 3:1 and 2:1 (w/w). The study showed that retention was a key indicator of bioavailability. The construction of mixing solid nanodispersions and their synergistic effect provide new theoretical and technological foundations for extending biopesticide lifetime and retarding resistance. The mixing nanoformulation has broad prospect in the field of agriculture.

Key words: emamectin benzoate, lambda-cyhalothrin, mixing solid nanodispersion, synergistic effect

中图分类号:

S482.3

崔博, 王春鑫, 吕妍, 姚俊伟, 刘国强, 崔海信. 两种杀虫剂复配的固体纳米分散体的增效研究[J]. 中国生物防治学报, 2017, 33(6): 760-766.

CUI Bo, WANG Chunxin, LÜ Yan, YAO Junwei, LIU Guoqiang, CUI Haixin. Synergistic Effect of Mixing Solid Nanodispersions of Two Insecticides[J]. journal1, 2017, 33(6): 760-766.

参考文献

[1] 陆长婴, 沈国清, 张燕, 等. 毒死蜱和甲氨基阿维菌素苯甲酸盐的联合增效作用机理研究[J]. 上海交通大学学报(农业科学版), 2009, 27(2):153-156.
[2] 谭明星, 孙光忠, 董兰. 2.5%甲氨基阿维菌素苯甲酸盐水分散粒剂对小菜蛾的防治效果[J]. 植物医生, 2010, 23(1):28-29.
[3] 毕富春, 徐凤波. 甲胺基阿维菌素苯甲酸盐研究概述[J]. 农药科学与管理, 2002, 23(3):31-33.
[4] 慕卫, 刘峰, 张文吉. 甲胺基阿维菌素对甜菜夜蛾、棉铃虫、粘虫和苜蓿夜蛾的活性研究[J]. 农药, 2002, 43(8):27-28.
[5] 佘佳荣, 杨仁斌, 杨周宁, 等. 高效氯氟氰菊酯水乳剂在小白菜和土壤中的残留动态研究[J]. 中国农学通报, 2010, 26(20):344-348.
[6] 徐汉虹. 植物化学保护学(第四版)[M]. 北京:中国农业出版社, 2007.
[7] Bouri M, Salghi R, Bazzi L, et al. Pesticide residue levels in green beans cultivated in Souss Masa Valley (Morocco) after multiple applications of bifenthrin and lambda-cyhalothrin[J]. Bulletin of Environmental Contamination and Toxicology, 2012, 89(3):638-643.
[8] George T, Beevi S N, Xavier G, et al. Dissipation kinetics and assessment of processing factor for chlorpyrifos and lambda-cyhalothrin in cardamom[J]. Environmental Monitoring and Assessment, 2013, 185(6):5277-5284.
[9] 范仁俊, 高越, 张润祥, 等. 2.5%高效氟氯氰菊酯微乳剂对苹果桃小食心虫的田间应用技术研究[J]. 应用昆虫学报, 2010, 47(2):384-386.
[10] 孟建玉, 陈兴江, 曹毅, 等. 贵州主要烟区烟蚜对灭多威和高效氯氟氰菊酯的抗性水平测定[J]. 河南农业科学, 2015, 44(1):82-85.
[11] 何翠娟, 毛明华, 赵胜荣. 高效氯氟氰菊酯和高效氯氰菊酯对蔬菜蚜虫、美洲斑潜蝇防治效果评价[J]. 世界农药, 2010, 32(2):36-40.
[12] 刘永杰, 沈晋良. 甜菜夜蛾对四类杀虫剂的抗药性监测[J]. 棉花学报, 2002, 14(6):356-360.
[13] 王军, 许培援, 邹文援, 等. 绿色农药剂型——微乳剂型[J]. 精细与专用化学品, 2004, 12(21):19-20.
[14] 成家壮. 我国农药剂型和制剂的可持续发展[J]. 广州化工, 2009, 37(7):3-7.
[15] 杨东升. 三种难溶性杀虫剂的纳米载药系统构建、表征及药效功能评价[D]. 北京:中国农业科学院, 2017.
[16] Knowles A. Recent developments of safer formulations of agrochemicals[J]. Environmentalist, 2008, 28(1):35-44.
[17] de Oliveira J L, Campos E V, Bakshi M, et al. Application of nanotechnology for the encapsulation of botanical insecticides for sustainable agriculture:prospects and promises[J]. Biotechnology Advances, 2014, 32(8):1550-1561.
[18] Kawakami K. Modification of physicochemical characteristics of active pharmaceutical ingredients and application of supersaturatable dosage forms for improving bioavailability of poorly absorbed drugs[J]. Advanced Drug Delivery Reviews, 2012, 64(6):480-495.
[19] Nkansah P, Antipas A, Lu Y, et al. Development and evaluation of novel solid nanodispersion system for oral delivery of poorly water-soluble drugs[J]. Journal of Controlled Release, 2013, 169(1/2):150-161.
[20] 武中平, 杨红, 林开春. 阿维菌素与高效氯氰菊酯复配制剂及其生物活性的研究[J]. 现代农药, 2002, 1(3):23-25.
[21] 娄殿国, 邢光耀. 不同杀菌剂和杀虫剂混配拌种对棉苗病害、棉蚜和绿盲蝽的效果[J]. 中国棉花, 2014, 41(3):27-29.
[22] 郭白洁, 彭建新, 蔡旭. 6%乙基多杀菌素和苏云金杆菌复配防治小菜蛾田间药效评价[J]. 福建农业科技, 2016, 47(7):22-24.
[23] 袁静, 郁季平, 陈杰, 等. 阿维菌素和环氧虫啶混配对水稻褐飞虱的协同杀虫作用[J]. 农药, 2014, 53(11):840-841.
[24] 任璐, 韩巨才. 甲胺基阿维菌素苯甲酸盐与高效氯氰菊酯混配对甜菜夜蛾的增效作用[J]. 山西农业大学学报(自然科学版), 2007, 27(4):427-428.
[25] 高越, 陆俊姣, 张润祥, 等. 3%高效氟氯氰菊酯与甲胺基阿维菌素苯甲酸盐对小菜蛾联合毒力测定[J]. 安徽农业科学, 2010, 38(31):17556-17557.
[26] Cui B, Feng L, Pan Z, et al. Evaluation of stability and biological activity of solid nanodispersion of lambda-cyhalothrin[J]. PLoS ONE, 2015, 10(8):e0135953.
[27] Cui B, Feng L, Wang C, et al. Stability and biological activity evaluation of chlorantraniliprole solid nanodispersions prepared by high pressure homogenization[J]. PLoS ONE, 2016, 11(8):e0160877.
[28] Feng L, Cui B, Yang D, et al. Preparation and evaluation of emamectin benzoate solid microemulsion[J]. Journal of Nanomaterials, 2016, 2016:2386938.
[29] 袁会珠, 齐淑华, 杨代斌. 药液在作物叶片的流失点和最大稳定持留量研究[J]. 农药学学报, 2000, 2(4):66-71.
[30] Sun Y P, Johnson E R. Analysis of joint action of insecticides against house flies[J]. Journal of Economic Entomology, 1960, 53(5):887-892.
[31] 胡美英, 黄炳球, 肖整玉, 等. 表面活性剂对杀虫剂的增效机制及药效研究[J]. 华南农业大学学报, 1998, 19(3):41-46.
[32] 屠豫钦. 农药剂型和制剂与农药的剂量转移[J]. 农药学学报, 1999, 1(1):1-6.
[33] 庞红宇, 黄琴, 马琛, 等. 接触角在农药喷雾中应用的试验研究[C]//江树人主编. 农药与环境安全国际会议. 北京:中国农业大学出版社, 2005, 414-419.
[34] 李彦蓉, 张宝俊, 韩巨才. 甲维盐和高效氟氯氰菊酯混配对小菜蛾的增效作用测定[J]. 农业技术与装备, 2011, 1:63-64.