对舞毒蛾高毒力Bt杀虫蛋白协同增效组合的筛选

doi:10.16409/j.cnki.2095-039x.2016.01.011

中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (1): 70-75.DOI: 10.16409/j.cnki.2095-039x.2016.01.011

对舞毒蛾高毒力Bt杀虫蛋白协同增效组合的筛选

杨帆^1,2, 杨素娟², 曹利军¹, 耿涌鑫¹, 张杰², 陈敏¹

1. 北京林业大学林学院/林木有害生物防治北京市重点实验室, 北京 100083;
2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193

收稿日期:2015-03-22 出版日期:2016-02-08 发布日期:2016-02-22
通讯作者: 陈敏, 张杰
作者简介:杨帆(1989-),男,硕士,E-mail:yfanbjfu@sina.com
基金资助:
国家"863"计划(2011AA100201、2013AA102701)

Screening of Synergistic Combinations of Bacillus thuringiensis Proteins with High Activity against Lymantria dispar

YANG Fan^1,2, YANG Sujuan², CAO Lijun¹, GENG Yongxin¹, ZHANG Jie², CHEN Min¹

1. Beijing Key Laboratory for Forest Pest Control/College of Forestry, Beijing Forestry University, Beijing 100083, China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2015-03-22 Online:2016-02-08 Published:2016-02-22

摘要/Abstract

摘要： 舞毒蛾Lymantria dispar(Linnaeus)是世界性的重要农林业害虫。研究Bt蛋白对舞毒蛾幼虫的毒杀作用,筛选出高毒力Bt蛋白及具有协同增效作用的蛋白组合,将为舞毒蛾的生物防治提供依据。本研究提取11种Bt蛋白对人工喂养的3日龄舞毒蛾幼虫进行初步筛选,初筛结果发现Cry1Ac、Cry2Ab、Cry1Ca、Vip3Aj 4种蛋白具有较高毒力。进而分别测定了这4种蛋白的LC₅₀;7 d的生测结果表明,杀虫毒力最高的为Cry1Ac蛋白,LC₅₀达到了2.47 μg/g;Cry2Ab和Cry1Ca两种蛋白的LC₅₀分别为29.19和42.45 μg/g,表现出了较强的杀虫活性;而Vip3Aj的LC₅₀为101.26 μg/g,具有中等毒力,这是首次发现该新基因表达产物具有杀虫活性。然后进一步将Cry1Ac与Cry2Ab、Cry1Ac与Cry1Ca、Cry1Ac与Vip3Aj、Cry2Ab与Cry1Ca按1:1混配,测定蛋白组合的杀虫活性并计算增效因子。结果发现,Cry1Ac+Cry2Ab混配蛋白LC₅₀达到0.64 μg/g,协同毒力指数最高,为7.098,具有很强的协同增效作用;Cry1Ac+Cry1Ca和Cry1Ac+Vip3Aj混配蛋白LC₅₀分别达到0.76和1.06 μg/g,协同毒力指数分别为6.162和4.539,也具有显著的增效作用。

关键词: 舞毒蛾, 苏云金芽胞杆菌, 杀虫蛋白, 毒力测定, 协同增效

Abstract: Lymantria dispar (Linnaeus) is an important agricultural and forestry pest in the world. With the aim of identifying Bt proteins and their synergistic combinations that would be useful in the management of L. dispar, 11 Bt proteins were extracted to test their toxicity against 3 d old larvae of L. dispar. Cry1Ac, Cry2Ab, Cry1Ca and Vip3Aj had high insecticidal activity in the primary screen. Further bioassay showed that Cry1Ac was the most toxic among the four proteins at 7 d treatment, with LC₅₀ of 2.47 μg/g. Cry2Ab and Cry1Ca also had a higher insecticidal activity, with LC₅₀ of 29.19 and 42.45 μg/g, respectively. The novel protein Vip3Aj was found for the first time to have pesticidal activity, with LC₅₀ of 101.26 μg/g. The larvicidal activities of four combinations (1:1 ratios) of the toxins (Cry1Ac+Cry2Ab, Cry1Ac+Cry1Ca, Cry1Ac+Vip3Aj, Cry2Ab+Cry1Ca) were evaluated. LC₅₀ of Cry1Ac+Cry2Ab combination was 0.64 μg/g, and the synergistic coefficient (7.098) was the highest, suggesting that there is strong synergism between these two toxins. With LC₅₀ of 0.76 and 1.06 μg/g, respectively, the Cry1Ac+Cry1Ca and Cry1Ac+Vip3Aj combinations, showed synergistic coefficients of 6.162 and 4.539,respectively, also indicating obvious synergistic effect.

Key words: Lymantria dispar, Bacillus thuringiensis, insecticidal proteins, bioassay, synergism

中图分类号:

S476.11

杨帆, 杨素娟, 曹利军, 耿涌鑫, 张杰, 陈敏. 对舞毒蛾高毒力Bt杀虫蛋白协同增效组合的筛选[J]. 中国生物防治学报, 2016, 32(1): 70-75.

YANG Fan, YANG Sujuan, CAO Lijun, GENG Yongxin, ZHANG Jie, CHEN Min. Screening of Synergistic Combinations of Bacillus thuringiensis Proteins with High Activity against Lymantria dispar[J]. journal1, 2016, 32(1): 70-75.

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对舞毒蛾高毒力Bt杀虫蛋白协同增效组合的筛选

Screening of Synergistic Combinations of Bacillus thuringiensis Proteins with High Activity against Lymantria dispar

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