对番茄枯萎病具防效的海洋源赤杆菌YH-07的发酵条件探究

doi:10.16409/j.cnki.2095-039x.2018.05.015

摘要/Abstract

摘要： 一株分离自连云港海域的赤杆菌Erythrobacter sp.YH-07对番茄枯萎病病原菌有较好的平板抑菌效果。通过单因子水平试验、正交试验对该菌株的培养基配方及摇瓶发酵条件进行优化，最优培养基配方为酵母浸膏0.25%、蔗糖1%、蛋白胨1%、NaCl 3%和MgSO₄·7H₂O 0.03%；最优发酵条件组合为培养温度30℃，140 r/min摇床培养，接种量1%，装液量50 mL/250 mL，初始pH 7.0。优化后的平板抑菌能力提高了9.4%。温室盆栽试验表明，赤杆菌YH-07对番茄枯萎病有显著抑制作用，防治效果保持在50%以上。同时显著降低番茄根际土的真菌数量，增加细菌和放线菌数量。综上所述，赤杆菌YH-07能有效防治番茄枯萎病害，改善根际微生物数量结构，具有进一步开发应用的前景。

关键词: 赤杆菌, 番茄枯萎病, 发酵条件优化, 拮抗作用, 生防效果

Abstract: A marine bacteria Erythrobacter sp. YH-07 with high biocontrol activity against Fusarium oxysporum was isolated from Lianyungang beach. We used single factor experiments and orthogonal experiments to investigate the best condition for fermentation of strain YH-07. The best composition of the medium was 0.25% (W/V) yeast extract, 1% (W/V) source, 1% (W/V) tryptone, 3% (W/V) NaCl and 0.03% (W/V) MgSO₄·7H₂O. The optimal fermentation conditions were the combination of temperature 30℃, initial pH7.0, 140 r/min, 1% inoculums volume percentage and medium volume 50 mL/250 mL. Under the optimum fermentation medium and culture conditions, the inhibitory rate of strain YH-07 was 9.4% higher than before. Pot experiments indicated that H7+R treatment could significantly lower the incidence rate of Fusarium wilt disease up to 50%, increase the total number of bacteria and actinomycetes, and decrease the number of fungi in rhizosphere soil. Strain YH-07 can effectively control Fusarium wilt of tomato and improve microbial community structure in rhizosphere, and it has potential development and application prospect.

Key words: Erythrobacter sp., tomato Fusarium wilt, optimization of fermentation condition, antagonism, biocontrol efficiency

中图分类号:

S476

唐彤彤, 孙星, 董元华, 刘勤. 对番茄枯萎病具防效的海洋源赤杆菌YH-07的发酵条件探究[J]. 中国生物防治学报, 2018, 34(5): 753-761.

TANG Tongtong, SUN Xing, DONG Yuanhua, LIU Qin. Optimization of Fermentation Conditions for Erythrobacter sp. YH-07 Suppressing Fusarium Wilt of Tomato[J]. journal1, 2018, 34(5): 753-761.

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