枯草芽胞杆菌YB-05与申嗪霉素复配防治小麦茎基腐病

doi:10.16409/j.cnki.2095-039x.2018.06.009

中国生物防治学报 ›› 2018, Vol. 34 ›› Issue (6): 866-872.DOI: 10.16409/j.cnki.2095-039x.2018.06.009

枯草芽胞杆菌YB-05与申嗪霉素复配防治小麦茎基腐病

张洁¹, 汤蒙蒙¹, 夏明聪¹, 林琪童², 孙润红¹, 杨艳艳¹, 杨丽荣¹, 李洪连²

1. 河南省农业科学院植物保护研究所/河南省农作物病虫害防治重点实验室/农业部华北南部农作物有害生物综合治理重点实验室/河南省作物保护国际联合实验室, 郑州 450002;
2. 河南农业大学植物保护学院, 郑州 450002

收稿日期:2018-04-27 出版日期:2018-12-08 发布日期:2018-12-08
通讯作者: 杨丽荣,副研究员,E-mail:luck_ylr@126.com;李洪连,教授,E-mail:honglianli@sina.com
作者简介:张洁,博士,助理研究员,E-mail:zhangjie656@126.com
基金资助:
国家重点研发计划（2017YFD0301104，2017YFD0201101）；国家公益性行业（农业）科研专项（201503112）

Combination of Bacillus Subtilis YB-05 and Shenqinmycin for Integrated Control of Wheat Crown Rot

ZHANG Jie¹, TANG Mengmeng¹, XIA Mingcong¹, LIN Qitong², SUN Runhong¹, YANG Yanyan¹, YANG Lirong¹, LI Honglian²

1. Institute of Plant Protection Research, Henan Academy of Agricultural Sciences/Henan Key Laboratory for Control of Crop Diseases and Insect Pests/IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture/International Joint Research Laboratory for Crop Protection of Henan, Zhengzhou 450002, China;
2. College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China

Received:2018-04-27 Online:2018-12-08 Published:2018-12-08

摘要/Abstract

摘要： 由假禾谷镰刀菌Fusarium pseudograminearum引起的小麦茎基腐病是一种重要的土传病害，生产上亟需一种安全有效的防控方法。本研究利用枯草芽胞杆菌YB-05与申嗪霉素复配，并评价该复配制剂对小麦茎基腐病的防治效果。结果显示当申嗪霉素浓度低于500 μg/mL时，对YB-05的菌落生长无显著影响；室内盆栽及田间试验表明枯草芽胞杆菌YB-05菌液和1%申嗪霉素悬浮剂按照19：1（vt/vt）复配生物制剂20 mL/kg拌种处理发病最轻，均超过了高剂量的单剂处理（枯草芽胞杆菌YB-05 30 mL/kg拌种处理和1%申嗪霉素2 mL/kg拌种处理）和4.8%适麦丹水悬浮剂2 mL/kg拌种处理，并且具有一定的促生和增产作用。其中，复配制剂室内防治效果为69.8%，在两次的田间调查中防治效果分别为57.8%和45.7%。由此可见，枯草芽胞杆菌与申嗪霉素复配具有协同增效的作用，可以减少化学农药的使用，具有较好的开发利用潜力。

关键词: 小麦茎基腐病, 假禾谷镰刀菌, 枯草芽胞杆菌, 申嗪霉素, 生物防治

Abstract: Wheat crown rot caused by Fusarium pseudograminearum is an important soil-brone disease and effective control measures are desperately needed. In this study, B. subtilis YB-05 and shenqinmycin were combined and the efficiency of biopesticides to control wheat crown rot was evaluated in the greenhouse and field conditions. The results showed that the colony growth of YB-05 was not impaired by 500 μg/mL shenqinmycin. Greenhouse and field trials demonstrated that treatments with the combination of B. subtilis YB-05 and shenqinmycin showed better control effect than that of the single biocontrol treatment and 4.8% myclobutanil. This treatment also promoted wheat growth and increased wheat yields. The control efficacy of the combined biopesticide was 69.8% in greenhouse, and 57.8% and 45.7% in the two surveys of field, respectively. It is concluded that the combination of B. subtilis YB-05 and shenqinmycin shows synergetic effect and has the potential to be developed as a commercial biopesticide agent aiming at less pesticide application.

Key words: wheat crown rot, Fusarium pseudograminearum, Bacillus subtilis, shenqinmycin, biological control

中图分类号:

S476

张洁, 汤蒙蒙, 夏明聪, 林琪童, 孙润红, 杨艳艳, 杨丽荣, 李洪连. 枯草芽胞杆菌YB-05与申嗪霉素复配防治小麦茎基腐病[J]. 中国生物防治学报, 2018, 34(6): 866-872.

ZHANG Jie, TANG Mengmeng, XIA Mingcong, LIN Qitong, SUN Runhong, YANG Yanyan, YANG Lirong, LI Honglian. Combination of Bacillus Subtilis YB-05 and Shenqinmycin for Integrated Control of Wheat Crown Rot[J]. journal1, 2018, 34(6): 866-872.

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枯草芽胞杆菌YB-05与申嗪霉素复配防治小麦茎基腐病

Combination of Bacillus Subtilis YB-05 and Shenqinmycin for Integrated Control of Wheat Crown Rot

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