恶臭假单胞菌HB3S-20对番茄青枯病的抑制作用及其抑菌机制初探

doi:10.16409/j.cnki.2095-039x.2026.02.034

摘要/Abstract

摘要： 番茄青枯病是全球危害最严重的细菌性病害之一。恶臭假单胞菌HB3S-20是从棉花内生组织中分离到的一株细菌，室内抑菌试验结果表明，该菌株对番茄青枯病菌具有显著的抑制作用，抑菌圈直径达28.9mm。生长特性研究表明，菌株HB3S-20的最适生长温度为30℃，最适pH值范围为pH 5.0～7.0，最适碳源和氮源分别为蔗糖和酵母提取物。功能分析结果显示，该菌株具有固氮、溶解无机磷和产生嗜铁素的能力，但不具备溶解有机磷和产生吲哚乙酸（IAA）的功能。盆栽试验的结果显示该菌株对番茄青枯病的防效为75.2%，进一步验证了其抑菌活性。利用GFP基因标记技术对菌株HB3S-20进行番茄根部的定殖观察发现，该菌株能够在番茄根部表面定殖，这为其发挥生防作用提供了基础。为进一步解析其抗菌机制，结合三代和二代全基因组测序技术对菌株HB3S-20进行全基因组测序，并通过antiSMASH在线平台预测到8个次级代谢产物合成基因簇，同源比对分析表明，其中部分基因簇可能参与viscosin和putisolvin等已知抗菌物质合成，此外还可能存在编码新型抗菌物质的基因簇。综上所述，恶臭假单胞菌HB3S-20具有良好的根际定殖能力和多重抑菌潜力，在番茄青枯病生物防治中具有良好的应用前景。

关键词: 恶臭假单胞菌HB3S-20, 生物学特性, 生物防治, 番茄青枯病, 定殖, 基因测序和分析

Abstract: Tomato bacteria wilt is one of the most serious bacterial diseases worldwide. Pseudomonas putida HB3S-20 was isolated from the endophytic tissues of cotton, the antibacterial activity test showed that the strain HB3S-20 exhibited significant antibacterial activity against tomato bacterial wilt and the diameter of the inhibitory zone was 28.9 mm. The growth characteristics study indicated that the optimal growth temperature of strain HB3S-20 was 30℃, and the optimal pH range was pH 5.0 to 7.0, and the most suitable carbon and nitrogen sources were sucrose and yeast extract respectively. The strain HB3S-20 was capable of fixing nitrogen, solubilizing inorganic phosphorus, and producing siderophore, while it could not dissolve organic phosphorus and produce indole acetic acid(IAA). The pot experiment showed that the control effect of strain HB3S-20 on tomato bacterial wilt was 75.2% to further verify its antibacterial activity. By using GFP gene labeling technique for colonization observation of strain HB3S-20 on tomato roots, the results indicated that this strain could colonize on the surface of tomato roots, providing a basis for its biological control effect. To further elucidate its antibacterial mechanism, the whole genome of strain HB3S-20 was sequenced using a combination of the third-generation and second-generation sequencing technology, eight secondary metabolite synthesis gene clusters were predicted through antiSMASH online platform. Homologous alignment analysis indicated that these gene clusters may be involved in the synthesis of two known types of antibacterial substances, viscosin and putisolvin. Additionally, there may be gene clusters encoding novel antibacterial substances. In summary, the strain HB3S-20 exhibited good colonization ability in tomato roots and multiple antibacterial potential, and has a promising application prospect in the biological control of tomato bacterial wilt.

Key words: Pseudomonas putida HB3S-20, biological characteristics, biocontrol, tomato bacteria wilt, colonization, gene sequencing and analysis

中图分类号:

S476

金利容, 王玲, 许冬, 杨妮娜, 尹海辰, 黄薇, 刘林芳, 万鹏. 恶臭假单胞菌HB3S-20对番茄青枯病的抑制作用及其抑菌机制初探[J]. 中国生物防治学报, 2026, 42(3): 637-647.

JIN Lirong, WANG Ling, XU Dong, YANG Nina, YIN Haichen, HUANG Wei, LIU Linfang, WAN Peng. Antibacterial Effect and Mechanism of Pseudomonas Putida HB3S-20 on Tomato Bacterial Wilt[J]. Chinese Journal of Biological Control, 2026, 42(3): 637-647.

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