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journal1 ›› 2016, Vol. 32 ›› Issue (1): 55-61.DOI: 10.16409/j.cnki.2095-039x.2016.01.009

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Population Genetic Structure Analysis of Insect Fungal Pathogen Causing Silkworm Grey Muscardine

ZHANG ShengLi1,2, CHEN Xue2, PU Shunchang1, XU Yanping2, HE Lingmin2, LI Zengzhi2, LUAN Fenggang3   

  1. 1. Institute of Biological Engineering, Bozhou Teachers College, Bozhou 236800, China;
    2. Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China;
    3. College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2015-03-23 Online:2016-02-08 Published:2016-02-22

Abstract: Fungal pathogen Isaria javanica can cause a low rate of silkworm grey muscardine. In this study, two I. javanica populations collected from Qianshan and Tongxiang were fingerprinting analyzed using ISSR markers. In the assayed 19 isolates, 120 discernible DNA bands ranging in size from 200 to 2000 bp were generated by 12 ISSR primers, and 93 of the bands (70.45%) were polymorphic. Nei's gene diversity (h) and Shannon's Information index (Is) showed a similar trend with the percentage of polymorphism at the population level. At the point of genetic similarity coefficient 0.76, the strains could be divided into 2 groups which correlated with the geographic origin of sampling. The result of 3-D principal coordinate analyses (PCA) based on ISSR data shared high similarity with UPGMA cluster analysis. The gene flow (Nm) between the two populations was 0.5678, and the genetic differentiation coefficient (Gst) was 0.3287, which indicates higher genetic diversity between populations than within populations. Single population clustering results showed that Qianshan population had an evident dominant group at the genetic similarity coefficient of 0.8, while Tongxiang population did not have a predominant lineage. Based on the analyses above, we can conclude that pathogen populations causing enzootic grey muscardine are geographically heterogenous, which may be an important factor for the maintenance of enzootic populations.

Key words: Bombyx mori, grey muscardine, Isaria javanica, enzootic, ISSR

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