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journal1 ›› 2016, Vol. 32 ›› Issue (1): 93-100.DOI: 10.16409/j.cnki.2095-039x.2016.01.014

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Cloning of tri Cluster and Analysis of tri Genes Expressions under Different Trichodermin-producing Conditions in Trichoderma brevicompactum

YUAN Xiaofeng, SHENTU Xuping, YU Xiaoping   

  1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine/College of Life Science, China Jiliang University, Hangzhou 310018, China
  • Received:2015-06-16 Online:2016-02-08 Published:2016-02-22

Abstract: Trichodermin is one of trichothecenes compounds, which has strongly antifungal activity on many kinds of plant pathogenic fungi. To preliminary clarify the function of tri cluster on trichodermin biosynthesis by Trichoderma brevicompactum 0248, the full length of tri cluster (24793 bp) was cloned. Sequence analysis of this cluster identified seven orthologs of T. brevicompactum tri genes. The influence of three carbon sources on the trichodermin production as well as the expression levels of tri genes in T. brevicompactum were also investigated. The result showed that the expression levels of tri genes under high trichodermin-producing condition by T. brevicompactum were much higher than that of trichodermin-nonproducing or low trichodermin-producing condition after 40 h incubation. The high expressions of tri genes in the glucose medium resulted in the accumulation of trichodermin after 40 h incubation. The expression level of tri gene was positively correlated with the yield of trichodermin. It provided a basis for further study the functional of tri genes in T. brevicompactum.

Key words: Trichoderma brevicompactum, trichodermin, tri cluster, carbon source, real-time quantitative PCR

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