Changes in Volatiles of Tea Shoots Damaged by Tea Green Leafhoppers and Their Attraction to Schizophragma parvula Ogloblin

doi:10.16409/j.cnki.2095-039x.2016.02.002

Abstract

Abstract: The volatile compounds from healthy intact tea shoots (ITS) and the tea green leafhopper Empoasca vitis (Göthe) damaged tea shoots (TGLDTS) were collected by simultaneous distillation extraction (SDE) method, and identified by gas chromatography-mass spectrometry and further confirmed by comparison with synthetic standard samples. Seventeen volatile components shared by both ITS and TGLDTS were identified, including Z-3-hexen-1-ol, E-2-hexenal, linalool, geraniol, β-ionone and nerolidol and other common aromatic compounds. Nine other components including Z-3-hexenyl butyrate were identified only from ITS; whereas, additional seven components including hexanal were detected only from TGLDTS samples. The total volatile contents from TGLDTS and ITS samples were estimated to be 17.84 and 3.6 times as much as the internal standard, respectively. E-2-hexenal, benzaldehyde, α-farnesene and methyl salicylate were four abundant volatile components from TGLDTS, accounting for 11.8% and 8.3% of total volatile contents of TGLDTS and ITS, respectively. Nineteen synthetic volatile components, identified from TGLDTS, each at a dosage of 10^-2 g/mL, were tested in the lab Y-tube olfactometer against a mymarid parasitoid (Schizophragma parvula Ogloblin). Our bioassay results clearly showed that four of the tested compounds from TGLDTS E-2-hexenal, benzaldehyde, α-farnesene and methyl salicylate were significantly attractive to the mymarid. The feeding damage by tea green leafhopper seemed to increase the releases of E-2-hexenal, benzaldehyde, α-farnesene and methyl salicylate that may act as synomone components attracting the egg parastoid of the tea green leafhopper, S. parvula.

Key words: tea green leafhopper, Schizophragma parvula, synomone, tea shoot volatiles, attraction

CLC Number:

S476.3

HAN Shanjie, PAN Cheng, HAN Baoyu. Changes in Volatiles of Tea Shoots Damaged by Tea Green Leafhoppers and Their Attraction to Schizophragma parvula Ogloblin[J]. journal1, DOI: 10.16409/j.cnki.2095-039x.2016.02.002.

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