The Genome Sequence of Trichoderma harzianum Th-33

doi:10.16409/j.cnki.2095-039x.2016.02.011

Abstract

Abstract: The whole genome of Trichoderma harzianum Th-33 was sequenced on a Hiseq2500 instrument. A total of 196 scaffolds were assembled and 10849 genes were predicted with an average length of 1776 bp (GenBank number:PRJNA272949). 6238 predicted genes were annotated in gene ontology (GO) and 6789 predicted genes were mapped to 279 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. KEGG enrichment analysis showed that the significantly enriched pathways were amino benzoate degradation pathway involving 232 genes and bisphenol degradation pathway involving 206 genes. Rfam was used for the RNA classification and predication of the genome. A total of 7123 genes were assigned to 25 categories, and the largest number of genes was involved in posttranslational modification, protein turnover, chaperones category. The mycoparasitism-related carbohydrate active enzymes, proteases and secondary metabolites-related genes in the T. harzianum, T. atroviride, T. virens and T. reesei genome were compared. The data in this study offer a better understanding of the biocontrol mechanism of Trichoderma and explore the functional genes in Trichoderma.

Key words: Trichoderma harzianum, whole genome, gene annotation, metabolic pathway, mycoparasitismrelated genes

CLC Number:

S476

SUN Qing, JIANG Xiliang, PANG Li, WANG Lirong, LI Mei. The Genome Sequence of Trichoderma harzianum Th-33[J]. journal1, DOI: 10.16409/j.cnki.2095-039x.2016.02.011.

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