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journal1 ›› 2017, Vol. 33 ›› Issue (4): 463-471.DOI: 10.16409/j.cnki.2095-039x.2017.04.005

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Cloning and Prokaryotic Expression of the Methyltransferase Gene GhBSMT1 from Gossypium hirsutum and Its Expression Profile Analysis

JING Weixia1,2, HUANG Xinzheng2, LIU Danfeng2, ZHANG Qiang2, KOU Junfeng2, QU Aijun1, ZHANG Yongjun2, GUO Yuyuan2   

  1. 1. College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China;
    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2017-03-18 Online:2017-08-08 Published:2017-08-11

Abstract: In this study, a novel benzoic acid/salicylic acid carboxyl methyltransferase gene was cloned from Gossypium hirsutum CCRI 12 by using RT-PCR and RACE-PCR, which contained 1280 bp and named as GhBSMT1. The transferase was predicted to have a molecular weight of 40.8 kD and isoelectric point of 6.12, which was unstable hydrophilic protein. A recombinant soluble protein with a size of about 41 kD was also obtained by prokaryotic expression. The real-time quantitative PCR analysis indicated that GhBSMT1 was constitutively expressed in leaves, stems and roots of cotton plants. The transcript accumulation of GhBSMT1 in leaves declined significantly when the leaves were treated with methyl salicylate (MeSA), whereas methyl jasmonate (MeJA) had no obvious influence on the expression of GhBSMT1. Moreover, the expressions of GhBSMT1 were reduced significantly after 6 h or 48 h feeding by Helicoverpa armigera larvae. In general, the GhBSMT1 is expressed in all tissues of cotton plants and regulated by MeSA and H. armigera infestation.

Key words: Gossypium hirsutum, benzoic acid/salicylic acid carboxyl methyltransferases, sequence characteristics, prokaryotic expression, tissue expression profile

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