The Expression of the Antibacterial Peptide Genes from Two Sibling Species of Tea Geometrid was Different in Resistance to EoNPV Infection

doi:10.16409/j.cnki.2095-039x.2017.04.006

Abstract

Abstract: Ectropis obliqua and Ectropis grisescens are two sibling species, which are severe defoliator pests in tea plantations. The mortality rate of E. obliqua and E. grisescens were 100% and 35.5% after infection with the E. obliqua nucleopolyhedrovirus (EoNPV) for 10 days, respectively. Three antimicrobial peptides genes, including gloverin, attacin and moricin, were cloned from the third-instar larvae of the two infected sibling species by an overlapping PCR method. Phylogenetic analysis suggested that Gloverin, Attacin, Moricin proteins of E. obliqua and E. grisescens were classified into an independent branch using a Neighbor-joining method, respectively. Further determination by qRT-PCR showed that the transcript levels of gloverin, attacin and moricin genes were up-regulated in the two sibling species after infection with EoNPV. The highest expression level of gloverin was detected at 12 h group, while moricin was expressed highest at 48 h group. The transcript levels of gloverin and moricin were significantly higher in E. grisescens than in E. obliqua. However, the transcript levels of attacin were changed at the different time of EoNPV infection between two sibling species, which indicates that gloverin, moricin and attacin are potentially associated with active resistance to virus infection. These results are of significance for further exploring the role of antimicrobial peptide genes in the interaction between insects and viruses.

Key words: Ectropis grisescens, Ectropis obliqua, EoNPV, antimicrobial peptide

CLC Number:

S435.711

MAO Tengfei, FU Jianyu, SUN Liang, ZHOU Xiaogui, BAI Jiahe, XIAO Qiang. The Expression of the Antibacterial Peptide Genes from Two Sibling Species of Tea Geometrid was Different in Resistance to EoNPV Infection[J]. journal1, 2017, 33(4): 472-480.

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