Protein Elicitor Hrip1 Functions in Controlling Barley Yellow Dwarf Virus in Wheat

doi:10.16409/j.cnki.2095-039x.2020.01.006

Abstract

Abstract: Wheat yellow dwarf disease is caused by barley yellow dwarf virus (BYDV), which is transmitted by wheat aphid and causing huge loss in agriculture. Nowadays, induced resistance has aroused great discussion as a new measure to control plant pathogens and herbivores. Protein elicitor Hrip1 induced defense response in various plants, leading to broad spectrum resistance. In this study, we evaluated the control efficiency of Hrip1 against BYDV. Wheat seeds and seedlings were soaked and sprayed with 30 μg/mL Hrip1, respectively, and then inoculated with BYDV. The control efficiency of Hrip1 against BYDV was over 50% at 14^th day after BYDV inoculation and over 30% at 21^th day after BYDV inoculation. Q-RT-PCR test showed that mRNA quantity of BYDV coat protein was less in Hrip1 treated wheat seedlings than that of control. EPG test showed that Schizaphis graminum performed longer time to find probe site on leaf surface and feeding site in phloem on Hrip1 treated wheat seedings. These results suggested that Hrip1 is efficient in inhibiting BYDV proliferation, and affecting feeding behavior of S. graminum, futher more reducing virus transmission ability of S. graminum. Additionally, Hrip1 weakened the symptom of dwarf and yellow in wheat seedlings caused by BYDV. In conclusion, Hrip1 could be a biological agent to integrately control BYDV.

Key words: Hrip1, BYDV, virus transimission ability, virus proliferation

CLC Number:

S476

LI Lin, WANG Shuangchao, YANG Xiufen, Frederic FRANCIS, QIU Dewen. Protein Elicitor Hrip1 Functions in Controlling Barley Yellow Dwarf Virus in Wheat[J]. Chinese Journal Of Biological Control, 2020, 36(1): 97-104.

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