Physicochemical Characteristics and Biocontrol Efficacy of Antagonistic Protein from Bacillus subtilis GB519

doi:10.16409/j.cnki.2095-039x.2020.05.024

Abstract

Abstract: The strain GB519 of Bacillus subtilis was isolated from the soil and showed significant antimicrobial activity against rice blast caused by Magnaporthe oryzae. In order to clarify the physicochemical characteristics of antagonistic crude protein of strain GB519, the effects of illumination, UV, temperature, pH and enzymes (proteinase K, trypsase and pepsase) on antimicrobial activity were analyzed. The results showed that antagonistic protein was stable in illumination, UV and three kinds of enzymes; Antimicrobial activity remained stable at 40-100℃ for 1 h and decreased by only 7.4% at 121℃ for 30 min, showing good resistance to high temperature; Antimicrobial activity remained stable from pH 2.0 to 10.0, and declined by 14.9% and 18.1% at pH 11.0 and pH 12.0, respectively. Antagonistic protein had antagonistic effect on multiple crop pathogens, especially among which the antimicrobial activity against Sclerotinia sclerotiorum was 72.7%. Under greenhouse conditions, the biocontrol efficacy against leaf blast reached 80.1%. Therefore, the B. subtilis GB519 could be developed as biocontrol potential bacteria for the control of rice blast.

Key words: Bacillus subtilis GB519, antagonistic protein, physicochemical properties, Magnaporthe oryzae

CLC Number:

S476

ZHU Feng, WANG Jichun, TIAN Chengli, QI Shanyan, WANG Dongyuan, REN Jinping, Wang Yishan. Physicochemical Characteristics and Biocontrol Efficacy of Antagonistic Protein from Bacillus subtilis GB519[J]. Chinese Journal Of Biological Control, 2020, 36(5): 778-785.

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