The Behavioral Responses of Pediobius Yunnanensis to the Chemical Cues Related to Its Host and Host Plant

doi:10.16409/j.cnki.2095-039x.2021.09.026

Abstract

Abstract: The chemical cues related to host plants and hosts play an important role in the host searching of parasitoids. The wasp Pediobius yunnanensis is the pupal parasitoid of pine borer, Dioryctria rubella. In the present study, a series of experiments were conducted to explore the behavioral responses of female parasitoids to the chemical cues related to its host and host plant. Using a Y-shaped olfactometer, the orientation behaviors of female parasitoids were investigated with regard to the volatiles from different treatments of pine cones (pupa-containing cone, cone containing empty puparium left after emergence, larva-damaged cone, and healthy cone), pine branches (pupa-containing branch, larva-damaged branch, the system branch of either pupa-containing cone or pupa-containing branch, and healthy branch), and larva frass (3^rd instar larva frass, the larva frass in the pupa-containing cone). In an open arena, the effect of walking trails of mature larvae on the searching behaviors of female parasitoids was evaluated. The behavioral responses of the females to the extract of cocoon or pupa of D. rubella were examined by dual-choice test in Petri dish. The results showed that the cones containing either pupa or empty puparium were highly attractive to the females in olfactometer bioassays, while no significant effects of either larva-damaged cone or healthy cone on the behaviors of this parasitoid were observed. The females displayed significantly positive responses to the pupa-containing branch, but not to the larva-damaged branch, the system branch of either pupa-containing cone or pupa-containing branch, and healthy branch. The parasitoids showed a preference for the volatiles of the frass inside the pupa-containing cone, but not for the third instar larva frass. The walking trails of the mature larva had no significant effects on the searching behaviors of the females in the open arena bioassay. In the Petri dish test, the cocoon extract of either methanol, dichloromethane or n-hexane, and the pupa extract of dichloromethane or n-hexane not only strongly attracted the female parasitoids, but also significantly prolonged its residence time. The results are expected to provide theoretical basis for revealing the chemical regulation mechanism for the host searching behaviors of this parasitoid and the biological control of D. rubella.

Key words: Pediobius yunnanensis, Dioryctria rubella, Pinus tabulaeformis, chemical cues, behavior response

CLC Number:

S476.3

CHENG Manman, WANG Miao, MA Mengran, WANG Shuxiang, CUI Jianzhou, LI Jiquan, ZHANG Guowei. The Behavioral Responses of Pediobius Yunnanensis to the Chemical Cues Related to Its Host and Host Plant[J]. Chinese Journal of Biological Control, 2021, 37(6): 1189-1196.

References

[1] Mair M M, Ruther J. Chemical ecology of the parasitoid wasp genus Nasonia (Hymenoptera,Pteromalidae)[J]. Frontiers in Ecology and Evolution, 2019, 7:184.
[2] Quilici S, Rousse P. Location of host and host habitat by fruit fly parasitoids[J]. Insects, 2012, 3:1220-1235.
[3] Glasser S K, Farzan S. Host-associated volatiles attract parasitoids of a native solitary bee, Osmia lignaria Say (Hymenoptera, Megachilidae)[J]. Journal of Hymenoptera Research, 2016, 51:249-256.
[4] Gontijo L, Cascone P, Giorgini M, et al. Relative importance of host and plant semiochemicals in the foraging behavior of Trichogramma achaeae, an egg parasitoid of Tuta absoluta[J]. Journal of Pest Science, 2019, 92:1479-1488.
[5] Chen C S, Zhao C, Wu Z Y, et al. Whitefly-induced tomato volatiles mediate host habitat location of the parasitic wasp Encarsia formosa, and enhance its efficacy as a bio-control agent[J]. Pest Management Science, 2021, 77:749-757.
[6] González J M, Cusumano A, Williams H J, et al. Behavioral and chemical investigations of contact kairomones released by the mud dauber wasp Trypoxylon politum, a host of the parasitoid Melittobia digitata[J]. Journal of Chemical Ecology, 2011, 37:629-639.
[7] Silva-torres C S A, Matthews R W, Ruberson J R, et al. Olfactory cues in host finding by Melittobia digitate (Hymenoptera:Eulophidae)[J]. Annals of the Entomological Society of America, 2005, 98(4):595-600.
[8] Filella I, Bosch J, Llusià J, et al. The role of frass and cocoon volatiles in host location by Monodontomerus aeneus, a parasitoid of megachilid solitary bees[J]. Environmental Entomology, 2011, 40(1):126-131.
[9] Roque-Romero L, Cisneros J, Rojas J C, et al. Attraction of Chelonus insularis to host and host habitat volatiles during the search of Spodoptera frugiperda eggs[J]. Biological Control, 2020, 140:104100.
[10] Zhu G, Pan L, Zhao Y, et al. Chemical investigations of volatile kairomones produced by Hyphantria cunea (Drury), a host of the parasitoid Chouioia cunea Yang[J]. Bulletin of Entomological Research, 2017, 107(2):234-240.
[11] Voss S C, Spafford H, Dadour I R. Host location and behavioural response patterns of the parasitoid, Tachinaephagus zealandicus Ashmead (Hymenoptera:Encyrtidae), to host and host-habitat odours[J]. Ecological Entomology, 2009, 34:204-213.
[12] Giunti G, Benelli G, Conte G, et al. VOCs-mediated location of olive fly larvae by the braconid parasitoid Psyttalia concolor:a multivariate comparison among VOC bouquets from three olive cultivars[J]. Biomed Research International, 2016, DOI:10.1155/2016/7827615.
[13] Avila G A, Withers T M, Holwell G I. Olfactory cues used in host-habitat location and host location by the parasitoid Cotesia urabae[J]. Entomologia Experimentalis et Applicata, 2016, 158:202-209.
[14] 吴磊,杨振德,玉舒中,等.微红梢斑螟的研究进展[J].仲恺农业工程学院学报, 2014, 27(4):66-70.
[15] 靳轩,马梦然,邢子龙,等.联峰山油松林微红梢斑螟的生物学特性及发生规律研究[J].林业与生态科学, 2019, 34(3):273-279.
[16] 崔明哲,董志明,由云生.微红梢斑螟生物学特性观察及化学防治试验[J].中国园艺文摘, 2015, 11:64-65.
[17] 党英侨,王小艺,杨忠岐.天敌昆虫在我国林业害虫生物防治上的研究进展[J].环境昆虫学报, 2018, 4(2):242-255.
[18] 马梦然,靳轩,崔建州,等.云南派姬小蜂的生物学特性[J].中国生物防治学报, 2019, 35(6):829-834.
[19] 马梦然,曹鹤,申家轩,等.云南派姬小蜂的发育起点温度和有效积温[J].植物保护, 2020, 46(3):194-197.
[20] 马梦然,刘程,曹鹤,等.云南派姬小蜂对微红梢斑螟不同日龄蛹的选择性及其后代发育表现[J].中国植保导刊, 2019, 39(12):20-22.
[21] 王薇,李继泉,王树香,等.茉莉酸诱导桑枝对桑天牛卵啮小蜂的引诱作用及桑枝挥发物组分分析[J].昆虫学报, 201659(9):997-1003.
[22] Peri E, Cusumano A, Agro A, et al. Behavioral response of the egg parasitoid Ooencyrtus telenomicida to host-related chemical cues in a tritrophic perspective[J]. BioControl, 2011, 56:163-171.
[23] Miresmailli S, Gries R, Gries G, et al. Population density and feeding duration of cabbage looper larvae on tomato plants alter the levels of plant volatile emissions[J]. Pest Management Science, 2011, 68:101-107.
[24] McCormick A C, Unsicker S B, Gershenzon J. The specificity of herbivore-induced plant volatiles in attracting herbivore enemies[J]. Trends in Plant Science, 2012, 17:303-310.
[25] Pashalidou F G, Gols R, Berkhout B W, et al. To be in time:egg deposition enhances plant-mediated detection of young caterpillars by parasitoids[J]. Oecologia, 2015, 177:477-486.
[26] Meents A K, Chen S P, Reichelt M, et al. Volatile DMNT systemically induces jasmonate-independent direct anti-herbivore defense in leaves of sweet potato (Ipomoea batatas) plants[J]. Scientific Reports, 2019, 9:17431.
[27] Giunti G, Campolo O, Caccamo P, et al. Volatile infochemicals from Rhyzopertha dominica larvae and larval feces involved in Theocolax elegans host habitat location[J]. Insects, 2021, 12(142):1-12.
[28] Chiu-Alvarado P, Barrera J F, Rojas J C. Attraction of Prorops nasuta (Hymenoptera:Bethylidae), a parasitoid of the coffee berry borer (Coleoptera:Curculionidae), to host-associated olfactory cues[J]. Annals of the Entomological Society of America, 2009, 102(1):166-171.
[29] Steidle J L M, Van Loon J J A. Dietary specialization and infochemical use in carnivorous arthropods:Testing a concept[J]. Entomologia Experimentalis et Applicata, 2003, 108:133-148.
[30] Granchietti A, Sacchetti P, Marzia Rosi C, et al. Fruit fly larval trail acts as a cue in the host location process of the pupal parasitoid Coptera occidentalis[J]. Biological Control, 2012, 61:7-14.
[31] Li M, Yang Y, Yao Y, et al. Isolation and identification of attractants from the pupae of three lepidopteran species for the parasitoid Chouioia cunea Yang[J]. Pest Management Science, 2020, 76(5):1920-1928.