Welcome to Chinese Journal of Biological Control,Today is

Chinese Journal of Biological Control ›› 2024, Vol. 40 ›› Issue (2): 299-309.DOI: 10.16409/j.cnki.2095-039x.2023.01.027

• RESEARCH REPORTS • Previous Articles    

Breeding of Butenyl-spinosyns High Yielding Strain by 60Co-NTG Compound Mutation and Its Insecticidal Activity

XU Zhouqin1,2, GUO Chao2, LI Jinping3, ZHANG Xiaoyao2, WANG Kehui2, HE Zhiping1, LIU Xingquan1, WANG Chao2   

  1. 1. College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China;
    2. Institute of Cereal&Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China;
    3. Beijing Plant Protection Station, Beijing 100029, China
  • Received:2023-02-10 Published:2024-04-28

Abstract: In order to obtain stable and high-yield butenyl-spinosyn-producing strains and determine its lethal efficiency against agricultural pests,a high-yield mutant strain ASAGF30A11 with stable genetic traits was obtained by compound mutagenesis of a parent strain Saccharopolyspora pogona ASAGF19 screened from the previous study using 60Co-γ-ray and nitrosoguanidine.The new strain increased yield by 61.26% compared with the parent strain,and the yield variation of 3 generations was less than 10%.The crude butenyl-spinosyn was purified from the fermentation broth of the high-yield strain,and then diluted into the mother liquor with a concentration of 1.5 g/L.The insecticidal activity against Thrips palmi Karny,aphids and Plutella xylostella was investigated and the LC50 was determined to be 1.92 mg/L,1.23 mg/L and 0.27 mg/L,respectively.By comparison,butenyl-spinosyn was of a higher lethal efficiency against T.palmi than spinosyn (LC50 0.58 mg/L) and spinetoram (LC50 0.64 mg/L),a higher lethal efficiency against aphids than spinosyn (LC50 72.60 mg/L) but equal efficiency with avermectin (LC50 0.14 mg/L),and a higher lethal efficiency against P.xylostella than spinosyn (LC50 2.9 mg/L) but lower efficiency than spinetoram (LC50 0.05 mg/L).This study explored the effect of compound mutagenesis using 60Co-γ-ray and nitrosoguanidine on S.pogona for the first time and measured the laboratory lethal efficiency of butenyl-spinosyn against three agricultural pests,which serves as part of the theoretical basis for the control of agricultural pests with new biological insecticides.

Key words: Saccharopolyspora pogona, 60Co-γ-ray, NTG, combined mutagenesis, butenyl-spinosyns

CLC Number: