Isolation and Identification of Trichoderma harzianum TW21990 and Its Control Effect on Strawberry Root Rot

doi:10.16409/j.cnki.2095-039x.2025.02.058

Abstract

Abstract: The aim of this study was to screen antagonistic Trichoderma strains with biocontrol effects against strawberry root rot in Licheng District, Jinan city. The pathogens causing strawberry root rot were identified based on morphology and ITS sequence. The antagonistic Trichoderma strains were screened against dominant pathogens with strong susceptibility, and the biocontrol effect of selected Trichoderma strains was evaluated by pot experiment and field test. The results showed that the dominant pathogens of strawberry root rot in Licheng District were Colletotrichum spp., among which a highly susceptible strain of CM9 was identified as C. siamense by polygene sequence characterization. Trichoderma harzianum TW21990 was selected for its excellent antagonistic activity by plate confrontation, with inhibition rates of volatile and soluble metabolites against C. siamense CM9 reaching 53.32% and 63.24%, respectively. Pot experiment and field test demonstrated that control efficacy of TW21990 against strawberry root rot was 61.14% and 60.70%, respectively. In addition TW21990 treatment significantly improved the quality of strawberry fruit. The content of vitamin C, soluble sugar and soluble solids in the fruit with increased by 32.89%, 32.06% and 11.96% respectively compared with the control group. In conclusion, TW21990 showed great application potential for strawberry production.

Key words: strawberry root rot, pathogen identification, Trichoderma harzianum TW21990

CLC Number:

S436.68

LI Hongmei, WEI Yanli, YANG Kai, HU Jindong, LI Yang, ZHAO Zhongjuan, LI Jishun, YANG Jianwen. Isolation and Identification of Trichoderma harzianum TW21990 and Its Control Effect on Strawberry Root Rot[J]. Chinese Journal of Biological Control, 2025, 41(5): 1179-1187.

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