Isolation and Identification of a Fluridone-Degrading Strain and Its Degradation Characteristics

doi:10.16409/j.cnki.2095-039x.2026.02.044

Abstract

Abstract: This study aims to use microbial remediation technology to reduce soil fluridone residues and alleviate its toxic effects on subsequent crops. Using cotton field soil with long-term exposure to fluridone in Xinjiang, this study screened a degrading strain capable of utilizing fluridone as its sole carbon source via enrichment culture method. Combined with morphological observation, physiological and biochemical determination and 16S rRNA gene sequence analysis, the strain was classified and identified, and its degradation characteristics were explored by single factor test. At the same time, the mitigation effect of the strain on wheat phytotoxicity was verified by pot experiment. The results showed that the strain F9-1 was identified as Acinetobacter oryzae. Under the condition of initial concentration of fluridone 100 mg/L, the optimal degradation parameters of the strain were inoculation amount of 5%, pH 7.0, and culture temperature of 35°C. Under these conditions, the degradation rate of fluridone could reach 79.62% after 7 days of culture. Pot experiments confirmed that the inoculation strain F9-1 could significantly reduce the albino index of wheat seedlings in contaminated soil and increase the total chlorophyll content of leaves. In summary, strain F9-1 enriched the microbial resources for fluridone degradation, and also laid a foundation for the subsequent excavation and application of degrading enzymes.

Key words: fluridone, degrading bacteria, Acinetobacter oryzae, biodegradation, degradation characteristics

CLC Number:

S476

LI Hong, NIU Xinxiang, WU Fengkang, SHENG Yue, YANG Hongmei, CHU Min, BAO Huifang, WANG Ning, ZHAN Faqiang, YANG Rong, LOU Kai, SHI Yingwu. Isolation and Identification of a Fluridone-Degrading Strain and Its Degradation Characteristics[J]. Chinese Journal of Biological Control, 2026, 42(4): 993-1005.

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