Ligands Binding Characteristics of Odorant Binding Proteins AlucOBP33 of Apolygus lucorum (Hemiptera:Miridae)

doi:10.16409/j.cnki.2095-039x.2019.06.011

Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (2): 203-210.DOI: 10.16409/j.cnki.2095-039x.2019.06.011

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Ligands Binding Characteristics of Odorant Binding Proteins AlucOBP33 of Apolygus lucorum (Hemiptera:Miridae)

SUN Xiaojie^1,2, LI Zibo², ZHANG Qiang², LIU Yunze^1,2, ZHENG Fangqiang¹, ZHANG Yongjun²

1. College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2019-06-11 Online:2020-04-08 Published:2020-04-16

Abstract

Abstract: To investigate the roles of odorant binding protein AlucOBP33 in chemoreception of Apolygus lucorum, the ligand binding characteristics of AlucOBP33 was studied in laboratory. The candidate AlucOBP33 was obtained from antennal transcriptome data of A. lucorum. The 3D model predicts that AlucOBP33 belongs to classic OBPs which contain six alpha helix and three pairs of cysteine. AlucOBP33 was cloned, prokaryotic expressed and further purified by using affinity chromatography. The fluorescence competitive binding experiment was performed to determine the binding abilities of AlucOBP33 to 73 odorant chemicals and 13 taste compounds. It was found that recombinant AlucOBP33 bound effectively with two alcohols, four ketones, one ester, one aldehyde, one alkene, and four taste compounds. We propose that AlucOBP33 play dual roles in olfactory perception and taste recognition of A. lucorum.

Key words: Apolygus lucorum, odorant binding protein, fluorescence competitive binding assays, chemoreception

CLC Number:

S476

SUN Xiaojie, LI Zibo, ZHANG Qiang, LIU Yunze, ZHENG Fangqiang, ZHANG Yongjun. Ligands Binding Characteristics of Odorant Binding Proteins AlucOBP33 of Apolygus lucorum (Hemiptera:Miridae)[J]. Chinese Journal Of Biological Control, 2020, 36(2): 203-210.

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Ligands Binding Characteristics of Odorant Binding Proteins AlucOBP33 of Apolygus lucorum (Hemiptera:Miridae)

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