Searching and Parasitism Behavior of Trichogramma chilonis Ishii on Vacuum-Packed and Refrigerated Rice Moth Eggs

doi:10.16409/j.cnki.2095-039x.2024.02.065

Abstract

Abstract: The eggs of Corcyra cephalonica serve as an ideal intermediate host for Trichogramma chilonis in indoor rearing systems. However, both the storage method and duration of the eggs significantly influence the host preference and parasitism behavior of the parasitoid. This study utilized a stereomicroscope and video recording system to observe and analyze the searching and parasitism behaviors of T. chilonis on C. cephalonica eggs stored under two conditions: regular refrigeration and vacuum-packed refrigeration. The findings revealed that for storage durations of 0, 3, 6, and 9 days, T. chilonis exhibited a decline in entry frequency into the egg areas. The highest total and single parasitism rates were recorded on day 6, reaching 7.65 and 6.44 eggs, respectively. By day 25, despite the highest entry frequency, the total and single parasitism rates decreased to 4.00 and 1.08 eggs, respectively. For storage durations of 15, 20, and 25 days, vacuum-packed refrigerated eggs required longer detection and parasitism times but resulted in higher numbers of parasitized eggs. Specifically, on day 15, the detection time was 20.37 seconds, with a parasitism rate of 2.24 eggs; on day 20, parasitism time increased to 50.27 seconds, with a rate of 1.95 eggs; and by day 25, the parasitism rate declined to 1.70 eggs. While no significant differences in parasitism rates were observed between the two storage methods during the initial 0–11 parasitism events, a clear preference for vacuum-packed refrigerated eggs emerged during the 12th and 13th events. Overall, the searching efficiency of T. chilonis’s decreased with prolonged storage time, and vacuum- packed refrigeration proved to be a superior method for preserving C. cephalonica eggs at later stages. These results highlight vacuum-packed refrigeration as a viable alternative to regular refrigeration for extending the shelf life of C. cephalonica eggs in biological control programs.

Key words: Trichogramma chilonis Ishii, rice moth eggs, vacuum-packed, refrigeration, search and parasitize

CLC Number:

S476

MEI Wenhao, LI Huan, FAN Rui, ZHU Wenya, WANG Yi, MA Ruiyan, ZHANG Ye. Searching and Parasitism Behavior of Trichogramma chilonis Ishii on Vacuum-Packed and Refrigerated Rice Moth Eggs[J]. Chinese Journal of Biological Control, 2024, 40(6): 1422-1429.

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