Progress and Prospects in Research and Application of Botanical Pesticide in China

doi:10.16409/j.cnki.2095-039x.2026.02.028

Abstract

Abstract: Botanical pesticides represent one of the important directions for the development of green pesticides. The status and progress in the research and application of botanical pesticide in China is systematically summarized in this review, which also analyzes the industry's development bottlenecks and proposes ten specific development pathways from the standpoint of creating new quality productive forces. These pathways include the effective and quick discovery of active compounds, target identification and mechanism analysis, the development of diverse activity evaluation methods, the exploration of new functions and product development, the application of synthetic biology, the research and development of novel nano-formulations, the construction of new application technologies, the expansion of botanical pesticides in non-agricultural fields, the upgrading and transformation of production enterprises, the strengthening of management and demonstration promotion, and ultimately offering a reference for the high-quality development of China's botanical pesticide industry.

Key words: botanical pesticides

CLC Number:

MA Zhiqing, WU Hua, LU Xiaopeng, REN Xingyu, WANG Kang, ZHANG Bin, YAN He, LEI Peng, LI Wenkui, FENG Juntao. Progress and Prospects in Research and Application of Botanical Pesticide in China[J]. Chinese Journal of Biological Control, 2026, 42(2): 360-370.

References

[1] Lu X, Liu J, Fu X, et al. Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine[J]. Ecotoxicology and Environmental Safety, 2021, 227: 112909.
[2] Qie X, Lu W, Aioub AA, et al. Insight into the detoxification of haedoxan A and the synergistic effects of phrymarolin I against Mythimna separata[J]. Industrial Crops and Products, 2020, 158: 112967.
[3] Wang M, Ren X, Wang L, et al. A functional analysis of mitochondrial respiratory chain cytochrome bc1 complex in Gaeumannomyces tritici by RNA silencing as a possible target of carabrone[J]. Molecular Plant Pathology, 2020, 21(12): 1529-1544.
[4] Li T, Zhang J, Ma S, et al. Identification and mechanism of insecticidal periplocosides from the root bark of Periploca sepium Bunge[J]. Pest Management Science, 2021, 77(4): 1925-1935.
[5] Xu D, Wei M, Peng S, et al. Cuminaldehyde in cumin essential oils prevents the growth and aflatoxin B1 biosynthesis of Aspergillus flavus in peanuts[J]. Food Control, 2021, 125: 107985.
[6] 孔祥晨曦, 汪成林, 裴雅琨, 等. 利用VIGS技术分析PlDIR1基因在透骨草木脂素生物合成中的作用[J]. 西北农业学报, 2025, 34(11): 2077-2085.
[7] 芦小鹏. 雷公藤次碱作用于黏虫鱼尼丁受体的分子机理研究[D]. 杨凌: 西北农林科技大学, 2023.
[8] Zhang X, Zuo Y, Liu R, et al. A key amino acid substitution of vacuolar-type H+-ATPases A subunit (VATP-A) confers selective toxicity of a potential botanical insecticide, periplocoside P (PSP), in Mythimna separata and Spodoptera exigua[J]. Insect Biochemistry and Molecular Biology, 2025, 179: 104277.
[9] 郭文慧. 天然产物白屈菜碱诱导植物抗病活性及作用机制研究[D]. 杨凌: 西北农林科技大学, 2022.
[10] Zhou H, Wan F, Guo F, et al. High value-added application of a renewable bioresource as acaricide: Investigation the mechanism of action of scoparone against Tetranychus cinnabarinus[J]. Journal of Advanced Research, 2022, 38: 29-39.
[11] Cao W, Yang J, Hong B. Discovery and engineering of the biosynthesis of rotenoids[J]. Nature Synthesis, 2026, 5: 103-116.
[12] Zhao X, Chen L, Huang Y, et al. Targeted metabolic engineering of key biosynthetic genes improves triptolide production in Tripterygium wilfordii hairy roots[J]. Plant Cell Reports, 2025, 44: 129.
[13] Wang P, Fan Z, Wei W, et al. Biosynthesis of the plant coumarin osthole by engineered Saccharomyces cerevisiae[J]. ACS Synthetic Biology, 2023, 12: 2455-2462.
[14] Qi F, Zhang W, Xue Y, et al. Microbial production of the plant-derived fungicide physcion[J]. Metabolic Engineering, 2022, 74: 130-138.
[15] Hou K, Yu W, Wang X, et al. Metabolic engineering of Saccharomyces cerevisiae for de novo dihydroniloticin production using novel CYP450 from neem (Azadirachta indica)[J]. Journal of Agricultural and Food Chemistry, 2022, 70: 3467-3476.
[16] Yu J, Cheng K, Qu S, et al. Genome‐ scale metabolic modeling guided escherichia coli engineering for de novo biosynthesis of chrysanthemic acid[J]. Advanced Science, 2026, 13: e12736.
[17] Kou C, Liu J, Yin X, et al. Efficient heterologous biosynthesis of verazine, a metabolic precursor of the anti-cancer drug cyclopamine, in Nicotiana benthamiana[J]. Plant Communications, 2024, 5: 100831.
[18] Zhang B, Chen M, Pu S, et al. Identification of secondary metabolites in Tripterygium wilfordii hairy roots and culture optimization for enhancing wilforgine and wilforine production[J]. Industrial Crops and Products, 2020, 148: 112276.
[19] 王少康. 透骨草木脂素生物合成途径中三个新CYP450酶的发现与功能研究[D]. 杨凌: 西北农林科技大学, 2025.
[20] Zhao C, Tao M, Xu L, et al. Silica-based nanodelivery systems loaded with matrine for the brown planthopper green control and rice growth promotion[J]. ACS Sustainable Chemistry & Engineering, 2023, 11(49): 17299-17309.
[21] Liu Y, Wang G, Qin Y, et al. Sustainable nano-pesticide platform based on Pyrethrins II for prevention and control Monochamus alternatus[J]. Journal of Nanobiotechnology, 2022, 20(1): 183.
[22] Li W K, Fu X Y, Wu Y Z, et al. Polydopamine-doped porous nano-CaCO3-supported green tablets for the humidity-responsive intelligent release of active ingredients and control of grain storage pests[J]. ACS Sustainable Chemistry & Engineering, 2024, 12(6): 2421-2429.
[23] 张正炜, 郗厚诚, 常文程, 等. 我国植物源农药商品化应用现状及产业发展建议[J]. 世界农药, 2020, 42(12): 6-15.
[24] 董小绮, 康兆勇, 刘胜男, 等. 新型植物源天然产物杀虫剂研究进展[J]. 农药学学报, 2023, 25(5): 969-989.
[25] Xiong X, Yao M, Fu L, et al. The botanical pesticide derived from Sophora flavescens for controlling insect pests can also improve growth and development of tomato plants[J]. Industrial Crops and Products, 2016, 92: 13-18.
[26] Hashemi S S. Effect of wood vinegar on the release of calcium, magnesium, and phosphorus from calcareous soils in different land uses[J]. Journal of Arid Land, 2025, 17(5): 680-695.
[27] Xu W, Jia X, Yang M, et al. Tea polyphenol self-assembly nanocomposite coating for fruit preservation[J]. ACS Nano, 2025, 19(31): 28146-28159.
[28] Liu Y, Liu Z, Wang Y, et al. Biocompatible κ-carrageenan gel films with clove essential oil nanoemulsion: optimization, properties, and strawberry preservation efficacy[J]. Food Hydrocolloid, 2026, 174: 112392.
[29] Qiao Y, Zhang M, Cao Y, et al. Postharvest sclerotinia rot control in carrot by the natural product hinokitiol and the potential mechanisms involved[J]. International Journal of Food Microbiology, 2022, 383: 109939.
[30] Ding X, Zhao Y, Chen X, et al. Chitosan/kudzu-based packaging films synergistically reinforced by paeonol@ ZIF-8 and Ag2CO3/Ag2O nano-heterojunctions for raspberry preservation[J]. Food Packaging and Shelf Life, 2025, 51: 101584.
[31] 孙政, 赖忠晓, 赵晓敏, 等. 渭北旱塬苹果病虫害全程生物防控技术应用效果评价[J]. 中国农业科学, 2023, 56(6): 1102-1112.
[32] 殷越, 侯燕华, 袁善奎. 我国生物农药产业发展现状及面临的问题[J]. 农药科学与管理, 2026, 47(2): 7-13.
[33] http: //www.chinapesticide.org.cn/zwb/dataCenter?hash=reg-info[DB].
[34] 张宏军, 陶岭梅, 刘学, 等. 我国生物农药登记管理情况分析[J]. 中国生物防治学报, 2022, 38(1): 9-17.
[35] 宋宝安. 新质生产力视域下农药科技创新与绿色发展[J]. 农药科学与管理, 2025, 46(8): 1-6.
[36] Hannigan G D, Prihoda D, Palicka A, et al. A deep learning genome-mining strategy for biosynthetic gene cluster prediction[J]. Nucleic Acids Research, 2019, 47: e110.
[37] Duan Z K, Wang X, Lian M Y, et al. Bioassay-guided and deepSAT-driven precise mining of monoterpenoid coumarin derivatives with antifeedant effects from the leaves of Ailanthus altissima[J]. Journal of Agricultural and Food Chemistry, 2024, 72: 10958-10969.
[38] Kim H W, Zhang C, Reher R, et al. DeepSAT: learning molecular structures from nuclear magnetic resonance data[J]. Journal of Cheminformatics, 2023, 15: 71.
[39] Yu W C, Yu Y L, Dong B C, et al. Targeted discovery of aromatic glycosides with dual detoxification effects via a highly customized molecular networking platform[J]. Cell Chemical Biology, 2026, 33: 132-144.
[40] Zhang H, Yao J, Xiao G, et al. Discovery of drug targets based on traditional chinese medicine microspheres (TCM-MPs) fishing strategy combined with bio-layer interferometry (BLI) technology[J]. Analytica Chimica Acta, 2024, 1305: 342542.
[41] Meng F, Zhang G, Xiao W, et al. MedMeta: An AI-enabled and genomics-based database for functional profiling of secondary metabolites in medicinal species[J/OL]. Engineering, https: //doi.org/10.1016/j.eng. 2025.09.007.
[42] Jia Y, Gao B, Tan J, et al. Deep contrastive learning enables genome-wide virtual screening[J]. Science, 2026, 391: eads9530.
[43] Yang Y, Chen W, Mei Z, et al. The multifaceted role of microRNA in medicinal plants[J]. Medicinal Plant Biology, 2025, 4(1): 1-5.
[44] Yu X, Liu J, Wang W, et al. Plant-derived peptides: from identification to agronomic applications[J]. Molecular plant, 2025, 18: 1963-1982.
[45] Sparks T C, Bryant R J. Impact of natural products on discovery of, and innovation in, crop protection compounds[J]. Pest Management Science, 2022, 78(2): 399-408.
[46] Ren X, Bai J, Han Y, et al. Carabrone inhibits Gaeumannomyces tritici growth by targeting mitochondrial complex I and destabilizing NAD?/NADH homeostasis[J]. PLoS Pathogens, 2025, 21: e1013567.
[47] Cui Z, Li C, Chen P, et al. An update of label-free protein target identification methods for natural active products[J]. Theranostics, 2022, 12: 1829-1854.
[48] Wang B, Liu Q, Zhao W, et al. Revolutionizing drug discovery from natural products: the roles of artificial intelligence and multi-omics in accelerating innovation[J/OL]. Acta Pharmaceutica Sinica B, https: //doi.org/10.1016/j.apsb.2025.12.030.
[49] Wu H, Xu S, Chen Y, et al. Phenazine-1-carboxamide from Streptomyces suppresses Phytophthora nicotianae via cdc48-targeted mitochondrial disruption[J]. Plant, Cell & Environment, 2026, 49(4): 2295-2310.
[50] Qin D Q, Zheng Q, Zhang P W, et al. Azadirachtin directly or indirectly affects the abundance of intestinal flora of Spodoptera litura and the energy conversion of intestinal contents mediates the energy balance of intestine-brain axis, and along with decreased expression CREB in the brain neurons[J].Pesticide Biochemistry and Physiology, 2021, 173: 104778.
[51] 张兴, 马志卿, 冯俊涛, 等. 植物源农药研究进展[J]. 中国生物防治学报, 2015, 31(5): 685-698.
[52] 张菁. 印楝素和喜树碱抑制果蝇雌性生殖干细胞增殖的作用机理[D]. 广州: 华南农业大学, 2021.
[53] 袁洋, 陈业, 刘洋, 等. 巴西含羞草化感作用及潜在除草活性化合物分离鉴定[J]. 植物保护, 2022, 48(3): 123-130.
[54] 周兵, 闫小红, 肖宜安, 等. 石菖蒲根茎挥发油化学成分及其除草活性研究[J]. 植物保护, 2019, 45(2): 102-108.
[55] Du T, Zhu W, Zhang C, et al. Bacteriostatic activity and resistance mechanism of Artemisia annua extract against Ralstonia solanacearum in pepper. Plants, 2025, 14(5): 651.
[56] Jiang Y, Ji X, Zhang Y, et al. Citral induces plant systemic acquired resistance against tobacco mosaic virus and plant fungal diseases[J]. Industrial Crops and Products, 2022, 183: 114948.
[57] Luo W, Wang K, Luo J, et al. Limonene anti-TMV activity and its mode of action[J]. Pesticide Biochemistry and Physiology, 2023, 194: 105512.
[58] Jiang Y, Pan X, Li Y, et al. Linalool induces resistance against tobacco mosaic virus in tobacco plants[J]. Plant Disease, 2023, 107(7): 2144-2152.
[59] 罗伟, 姜悦, 王恺悦, 等. 紫苏精油中紫苏醛的抗TMV活性及作用方式(英文)[J]. 农药学学报, 2024, 26(4): 744-756.
[60] 郭文慧, 刘斌, 闫合, 等. 114种植物提取物诱导烟草抗烟草花叶病毒活性鉴定[J]. 中国生物防治学报, 2020, 36(4): 604-610.
[61] 孙雨晴. 烟草顺—冷杉醇制备及其对番茄和烟草青枯病的诱导抗性研究[D]. 北京: 中国农业科学院, 2023.
[62] 余峰, 李洁, 黎妍妍, 等. 橙皮素对烟草青枯病的诱导抗性研究[J]. 中国烟草科学, 2022, 43(4): 55-61.
[63] 杨晓聪, 摆俊龙, 洪波, 等. 小檗碱诱导拟南芥抗病信号通路检测与验证[J]. 西北农林科技大学学报, 2025, 53(12): 119-127,136.
[64] 王娅, 郭小秋, 郭声鑫, 等. 基于天然产物结构的植物诱抗剂创制[J]. 世界农药, 2025, 47(2): 15-25,61.
[65] Kutasy-Takacs B, Pallos J P, Kiniczky M, et al. Plant-derived biostimulants and liposomal formulations in sustainable crop protection and stress tolerance[J]. Applied Sciences, 2026, 16(1): 490.
[66] Arif Y, Bajguz A, Hayat S. Moringa oleifera extract as a natural plant biostimulant[J]. Journal of Plant Growth Regulation, 2023, 42(3): 1291-1306.
[67] Singh P, Varshney V K, Pattanaik S, et al. Moringa leaf extract as biostimulant to increase the reserpine and ajmaline level in commercially important Rauvolfia serpentina roots[J]. South African Journal of Botany, 2026, 189: 455-465.
[68] 王凤进. 100种植物提取物对辣椒和玉米生长刺激作用的筛选与评价[D]. 杨凌: 西北农林科技大学, 2022.
[69] 王宇萧. 两种天然生物刺激素对不结球白菜生长和品质的调控作用[D]. 南京: 南京农业大学, 2023.
[70] Bai J, Yang X, Jia Y, et al. Natural product chelerythrine induces plant resistance through activating defensive reactions[J]. Pest Management Science. 2025, 81(12): 7896-7906.
[71] Xu X, Qin D, Qin X, et al. Sustainable management of soil-borne disease: integrating fumigation with Andrographis paniculata residues to rebuild rhizosphere function[J]. BMC microbiology, 2025, 25(1): 460.
[72] Zhou J, Liu G Y, Guo Z J, et al. Stimuli-responsive pesticide carriers based on porous nanomaterials: A review[J]. Chemical Engineering Journal, 2023, 455: 140167.
[73] Li W K, Wang S, Wang Y H, et al. Nanoporous 3D polyurethane for toosendanin adsorption, encapsulation, and high-efficient utilization[J]. Journal of Agricultural and Food Chemistry, 2025, 73: 4574-4584.
[74] Shangguan W J, Huang Q L, Chen H P, et al. Making the complicated simple: a minimizing carrier strategy on innovative nanopesticides[J]. Nano-Micro Letters, 2024, 16: 193.
[75] 钱鑫, 沈杰. 基于星状多聚阳离子纳米载体的纳米生物农药创制与应用[J]. 中国生物防治学报, 2025, 41(5): 991-997.
[76] Zhang X H, Xiao J H, Huang Y Q, et al. Sustainable pest management using plant secondary metabolites regulated azadirachtin nano-assemblies[J]. Nature Communications, 2025, 16: 1721.
[77] Wang S, Zhan C, Chen R, et al. Achievements and perspectives of synthetic biology in botanical insecticides[J]. Journal of Cellular Physiology, 2024, 239: e30888.
[78] Li F, Yuan Z, Gao Y, et al. A concise enzyme cascade enables the manufacture of natural and halogenated protoberberine alkaloids[J]. Nature Communications, 2025, 16: 1904.
[79] Ko Y S, Kim J W, Lee J A, et al. Tools and strategies of systems metabolic engineering for the development of microbial cell factories for chemical production[J]. Chemical Society Reviews, 2020, 49: 4615-4636.
[80] Lisi F, Siscaro G, Biondi A, et al. Non-target effects of bioinsecticides on natural enemies of arthropod pests[J]. Current Opinion in Environmental Science & Health, 2025, 45: 100624.
[81] 孟涵. 农户玉米生产全周期生态效率评价及优化[D]. 北京: 中国农业科学院, 2025.
[82] 王倩倩, 孙玥, 龙瑞雪, 等. 牛至精油基抑菌膜在生鲜宁夏滩羊肉保鲜中的应用[J]. 食品科学, 2026, 47(1): 249-257.
[83] 邹颖, 寸金玉, 彭芍丹, 等. 高良姜精油可食膜的制备及其在鲜切菠萝保鲜中的应用[J]. 热带作物学报, 2025, 46(5): 1249-1258.
[84] 袁海彬, 董威, 陈绍飞, 等. 肉桂精油-细菌纤维素微囊体系的制备及其对蜜梨的保鲜作用[J]. 中国食品学报, 2025, 25(10): 254-263.
[85] 徐德峰, 廖威龙, 何嘉欣, 等. 植物精油在冷藏对虾保鲜中的应用进展[J]. 广东海洋大学学报, 2025, 45(5): 97-105.
[86] 刘绪, 曾晨阳, 张承燚, 等. 羧甲基壳聚糖负载复合精油膜延长牛肉货架期研究[J]. 食品与发酵工业, 2026, 52(6): 238-245.
[87] 翁佳丽, 张维, 于华. 纺织品用长效释放生物质精油微胶囊[J]. 精细化工, 2022, 39(12): 2561-2568, 2592.
[88] 贺楠, 马小强, 余炳根, 等. 超临界CO2提取柑橘精油及其对棉织物的驱蚊性能研究[J]. 纺织工程学报, 2025, 3(2): 24-35.
[89] 肖汉森, 何亚明, 季恒青. 灭蟑饵剂的研究及应用进展[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 642-646.
[90] Chellappandian M, Vasantha-Srinivasan P, Senthil-Nathan S, et al. Botanical essential oils and uses as mosquitocides and repellents against dengue[J]. Environment International, 2018, 113: 214-230.
[91] Jabeen A, Zaitoon A, Lim L, et al. Toxicity of five plant volatiles to adult and egg stages of drosophila Suzukii matsumura (Diptera: Drosophilidae), the spotted-wing drosophila. Journal of Agricultural and Food Chemistry, 2021, 69(33): 9511-9519.
[92] 曹梦茹, 付彤, 汪锦焕, 等. 智能家庭AI系统设计[J]. 智能处理与应用, 2026, 5: 108-112.
[93] 张涵, 孙书冬, 于志明. α-蒎烯精油缓释型水性丙烯酸多功能环保涂料[J]. 北京林业大学学报, 2025, 47(6): 162-172.
[94] 刘旖旎, 刘芳, 王德宝, 等. 纳米抗菌纤维的静电纺丝制备技术及其抗菌活性研究进展[J]. 中国食品学报, 2021, 21(12): 358-368.
[95] 安高锐, 阎继鹏, 孙剑. 纤维素基抗菌材料新进展：制备、机理和应用[J]. 中国造纸, 2026, 45(2): 189-203.
[96] 胡海玥, 刘梓檀, 张子震, 等. 天然生物大分子基食品级包装材料的研究进展[J]. 中国食品添加剂, 2025, 36(11): 151-159.
[97] 樊梓萱, 李松, 刘洋, 钟武. 微反应器在药物智能制造中的应用和发展前景[J]. 药学学报, 2026, (2026-03-09)[2026-05-06], DOI: 10.16438/ j.0513-4870.2025-1381.
[98] 黄修柱. 我国农药产业发展现状分析与展望[J]. 农药科学与管理, 2026, 47(1): 1-3, 37.