Antibacterial Effect and Mechanism of Pseudomonas Putida HB3S-20 on Tomato Bacterial Wilt

doi:10.16409/j.cnki.2095-039x.2026.02.034

Abstract

Abstract: Tomato bacteria wilt is one of the most serious bacterial diseases worldwide. Pseudomonas putida HB3S-20 was isolated from the endophytic tissues of cotton, the antibacterial activity test showed that the strain HB3S-20 exhibited significant antibacterial activity against tomato bacterial wilt and the diameter of the inhibitory zone was 28.9 mm. The growth characteristics study indicated that the optimal growth temperature of strain HB3S-20 was 30℃, and the optimal pH range was pH 5.0 to 7.0, and the most suitable carbon and nitrogen sources were sucrose and yeast extract respectively. The strain HB3S-20 was capable of fixing nitrogen, solubilizing inorganic phosphorus, and producing siderophore, while it could not dissolve organic phosphorus and produce indole acetic acid(IAA). The pot experiment showed that the control effect of strain HB3S-20 on tomato bacterial wilt was 75.2% to further verify its antibacterial activity. By using GFP gene labeling technique for colonization observation of strain HB3S-20 on tomato roots, the results indicated that this strain could colonize on the surface of tomato roots, providing a basis for its biological control effect. To further elucidate its antibacterial mechanism, the whole genome of strain HB3S-20 was sequenced using a combination of the third-generation and second-generation sequencing technology, eight secondary metabolite synthesis gene clusters were predicted through antiSMASH online platform. Homologous alignment analysis indicated that these gene clusters may be involved in the synthesis of two known types of antibacterial substances, viscosin and putisolvin. Additionally, there may be gene clusters encoding novel antibacterial substances. In summary, the strain HB3S-20 exhibited good colonization ability in tomato roots and multiple antibacterial potential, and has a promising application prospect in the biological control of tomato bacterial wilt.

Key words: Pseudomonas putida HB3S-20, biological characteristics, biocontrol, tomato bacteria wilt, colonization, gene sequencing and analysis

CLC Number:

S476

JIN Lirong, WANG Ling, XU Dong, YANG Nina, YIN Haichen, HUANG Wei, LIU Linfang, WAN Peng. Antibacterial Effect and Mechanism of Pseudomonas Putida HB3S-20 on Tomato Bacterial Wilt[J]. Chinese Journal of Biological Control, 2026, 42(3): 637-647.

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