Molecular Cloning and Expression Pattern Analysis of Peptidoglycan Recognition Protein Gene in the Green Leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae)

doi:10.16409/j.cnki.2095-039x.2017.03.002

Abstract

Abstract: To determine the function of peptidoglycan recognition protein in Nephotettix cincticeps (NcPGRP), we cloned the open reading frame cDNA nucleotide sequence of NcPGRP. The sequence is 552 bp in length, encoding a 19.9 kD protein but without signal peptide. NcPGRP has three Zn²⁺-binding and amidase catalyzing sites and one DAP-type peptidoglycan recognition site. Polygenetic tree analysis did not cluster NcPGRP and PGRPs of other species into one group. The real-time PCR results indicated that transcription level of NcPGRP was up regulated after injection of Escherichia coli DH_5α and Micrococcus luteus. Expression of NcPGRP mRNA could be detected in all tissues including head, gut, fat body, Malpighian tubes, ovary, testis and cuticle, but was the highest in the head. The transcription level of NcPGRP was down regulated in RDV-infected planthopper. The results suggest that RDV-infected N. cincticeps exhibits a lower resistance to pathogenic bacteria infection, which may be conductive to the symbiosis between N. cincticeps and RDV.

Key words: Nephotettix cincticeps, peptidoglycan recognition protein (PGRP), Rice dwarf virus (RDV), expression pattern, immunity

CLC Number:

HUANG Tianyu, FANG Qi, WANG Guirong, LIN Kejian, YE Gongyin. Molecular Cloning and Expression Pattern Analysis of Peptidoglycan Recognition Protein Gene in the Green Leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae)[J]. journal1, 2017, 33(2/3): 304-312.

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