The Research Progress of Secondary Metabolite Xcn1 in Xenorhabdus nematophila

doi:10.16409/j.cnki.2095-039x.2020.01.001

Abstract

Abstract: Xenocoumacin1 (Xcn1), a kind of water-soluble peptide antimicrobial compounds from Xenorhabdus nematophila, shows wide-spectrum antimicrobial activity towards bacteria and fungi, demonstrating great application potential in crop disease control. In this paper, we summarized the research progress of this compound in synthesis, degradation, regulation and crop disease control, and pointed out the possible strategies for strain engineering and industrial production.

Key words: Xenorhabdus nematophila, metabolite product, Xcn1, disease control

CLC Number:

S476

LI Guangyue, YANG Xiufen. The Research Progress of Secondary Metabolite Xcn1 in Xenorhabdus nematophila[J]. Chinese Journal of Biological Control, 2020, 36(1): 1-8.

References

[1] Poinar Jr G O. Taxonomy and biology of Steinernematidae and Heterorhabditidae[M]//Gaugler R, Kaya H K. Entomopathogenic Nematodes in Biological Control, Boca Raton:CRC Press Inc, 1990.
[2] Ciche T A, Ensign J C. For the insect pathogen Photorhabdus luminescens, which end of a nematode is out?[J]. Applied and Environmental Microbiology. 2003, 69(4):1890-1897.
[3] Forst S, Nealson K. Molecular biology of the symbiotic pathogenic bacteria Xenorhabdus spp. and Photorhabdus spp.[J]. Microbiological reviews, 1996, 60(1):21-43.
[4] Schubert E, Vetter I R, Prumbaum D, et al. Membrane insertion of α-xenorhabdolysin in near-atomic detail[J]. Elife, 2018, 7:e38017.
[5] Dal Peraro M, Van Der Goot F G. Pore-forming toxins:ancient, but never really out of fashion[J]. Nature Reviews Microbiology, 2016, 14(2):77-92.
[6] Akhurst R J, Dunphy G B. Tripartite interactions between symbiotically associated entomopathogenic bacteria, nematodes, and their insect hosts[M]//Beckage N E, Thompson S N, Federici B A, eds. Parasites and Pathogens of Insects. London:Academic Press, 1993.
[7] Bird A, Akhurst R. The nature of the intestinal vesicle in nematodes of the family Steinernematidae[J]. International Journal for Parasitology, 1983, 13(6):599-606.
[8] Goodrich-Blair H, Clarke D J. Mutualism and pathogenesis in Xenorhabdus and Photorhabdus:two roads to the same destination[J]. Molecular Microbiology, 2007, 64(2):260-268.
[9] Akhurst R. Antibiotic activity of Xenorhabdus spp., bacteria symbiotically associated with insect pathogenic nematodes of the families Heterorhabditidae and Steinernematidae[J]. Microbiology, 1982, 128(2):3061-3065.
[10] Sicard M, Brugirard-Ricaud K, Pagès S, et al. Stages of infection during the tripartite interaction between Xenorhabdus nematophila, its nematode vector, and insect hosts[J]. Applied and Environmental Microbiology, 2004, 70(11):6473-6480.
[11] Herbert E E, Goodrich-Blair H. Friend and foe:the two faces of Xenorhabdus nematophila[J]. Nature Reviews Microbiology, 2007, 5(8):634-646.
[12] Shi Y M, Bode H B. Chemical language and warfare of bacterial natural products in bacteria-nematode-insect interactions[J]. Natural Product Reports, 2018, 35(4):309-335.
[13] Brachmann A O, Bode H B. Identification and bioanalysis of natural products from insect symbionts and pathogens[M]//Vilcinskas A, ed. Yellow Biotechnology I. Advances in Biochemical Engineering/Biotechnology. Berlin, Heidelberg:Springer, 2013, 123-155.
[14] Challinor V L, Bode H B. Bioactive natural products from novel microbial sources[J]. Annals of the New York Academy of Sciences, 2015, 1354(1):82-97.
[15] 肖尧, 杨秀芬, 杨怀文. 致病杆菌和发光杆菌抗菌代谢产物研究进展[J]. 中国生物防治学报, 2011, 27(4):553-558.
[16] Chaston J M, Suen G, Tucker S L, et al. The Entomopathogenic bacterial endosymbionts Xenorhabdus and Photorhabdus:convergent lifestyles from divergent genomes[J]. PLoS ONE, 2011, 6(11):e27909.
[17] McInerney B V, Taylor W C, Lacey M J, et al. Biologically active metabolites from Xenorhabdus spp., Part 2. Benzopyran-1-one derivatives with gastroprotective activity[J]. Journal of Natural Products, 1991, 54(3):785-795.
[18] Yang X F, Qiu D W, Yang H W, et al. Antifungal activity of xenocoumacin 1 from Xenorhabdus nematophilus var. pekingensis against Phytophthora infestans[J]. World Journal of Microbiology and Biotechnology, 2011, 27(3):523-528.
[19] 黄武仁, 杨秀芬, 杨怀文, 等. 嗜线虫致病杆菌的抑菌物质及抑菌活性[J]. 天然产物研究与开发, 2006, 18:25-28.
[20] Reimer D, Luxenburger E, Brachmann A O, et al. A new type of pyrrolidine biosynthesis is involved in the late steps of xenocoumacin production in Xenorhabdus nematophila[J]. ChemBioChem, 2009, 10(12):1997-2001.
[21] Reimer D, Pos K M, Thines M, et al. A natural prodrug activation mechanism in nonribosomal peptide synthesis[J]. Nature Chemical Biology, 2011, 7(12):888-890.
[22] Park D, Ciezki K, van der Hoeven R, et al. Genetic analysis of xenocoumacin antibiotic production in the mutualistic bacterium Xenorhabdus nematophila[J]. Molecular Microbiology, 2009, 73(5):938-949.
[23] Park D, Forst S. Co-regulation of motility, exoenzyme and antibiotic production by the EnvZ-OmpR-FlhDC-FliA pathway in Xenorhabdus nematophila[J]. Molecular Microbiology, 2006, 61(6):1397-1412.
[24] Zhang S J, Fang X L, Tang Q, et al. CpxR negatively regulates the production of xenocoumacin 1, a dihydroisocoumarin derivative produced by Xenorhabdus nematophila[J]. MicrobiologyOpen, 2019, 8(1):e00674.
[25] Guo S Q, Zhang S J, Fang X L, et al. Regulation of antimicrobial activity and xenocoumacins biosynthesis by pH in Xenorhabdus nematophila[J]. Microbial Cell Factories, 2017, 16(1):203-206.
[26] Wang Y H, Fang X L, Cheng Y P, et al. Manipulation of pH shift to enhance the growth and antibiotic activity of Xenorhabdus nematophila[J]. Journal of Biomedicine and Biotechnology, 2011, 2011:1-9.
[27] Zhou T T, Yang X F, Qiu D W, et al. Inhibitory effects of xenocoumacin 1 on the different stages of Phytophthora capsici and its control effect on Phytophthora blight of pepper[J]. BioControl, 2017, 62(2):151-160.
[28] Zhou T, Zeng H, Qiu D, et al. Global transcriptional responses of Bacillus subtilis to xenocoumacin 1[J]. Journal of Applied Microbiology, 2011, 111(3):652-662.
[29] Engel Y, Windhorst C, Lu X J, et al. The global regulators Lrp, LeuO, and HexA control secondary metabolism in Entomopathogenic bacteria[J]. Frontiers in Microbiology, 2017, 8:1-13.
[30] Jubelin G, Lanois A, Severac D, et al. FliZ is a global regulatory protein affecting the expression of flagellar and virulence genes in individual Xenorhabdus nematophila bacterial cells[J]. PLoS Genetics, 2013, 9(10):e1003915.
[31] 李忝珍. 渗透压对嗜线虫致病杆菌YL001生长及抑菌活性物质产生的影响[D]. 杨凌:西北农林科技大学, 2017.
[32] 郭树奇, 许鹏, 鲁梅, 等. 初始pH对嗜线虫致病杆菌YLO01菌株抑菌活性及代谢的影响[J]. 西北农林科技大学学报(自然科学版), 2016, 44:132-140.
[33] Tran E E H, Andersen A W, Goodrich-Blair H. CpxRA influences Xenorhabdus nematophila colonization initiation and outgrowth in Steinernema carpocapsae nematodes through regulation of the nil locus[J]. Applied and Environmental Microbiology, 2009, 75(12):4007-4014.
[34] Tran E E H, Goodrich-Blair H. CpxRA contributes to Xenorhabdus nematophila virulence through regulation of lrhA and modulation of insect immunity[J]. Applied and Environmental Microbiology, 2009, 75(12):3998-4006.
[35] Forst S, Boylan B. Characterization of the pleiotropic phenotype of an ompR strain of Xenorhabdus nematophila[J]. Antonie van Leeuwenhoek, 2002, 81(43):1-4.
[36] Casanova-Torres A M, Shokal U, Morag N, et al. The global transcription factor Lrp is both essential for and inhibitory to Xenorhabdus nematophila insecticidal activity[J]. Applied and Environmental Microbiology, 2017, 83(12):e00185.
[37] Hussa E A, Casanova-Torres A M, Goodrich-Blair H. The global transcription factor Lrp controls virulence modulation in Xenorhabdus nematophila[J]. Journal of Bacteriology, 2015, 197(18):3015-3025.
[38] Cowles K N, Cowles C E, Richards G R, et al. The global regulator Lrp contributes to mutualism, pathogenesis and phenotypic variation in the bacterium Xenorhabdus nematophila[J]. Cellular microbiology, 2007, 9(5):1311-1323.
[39] 庞在堂, 杨怀文, 杨秀芬, 等. 一株高毒力致病杆菌CB6的鉴定[J]. 微生物学报, 2004, 44(2):131-135.
[40] 黄武仁, 朱昌雄, 杨秀芬, 等. 嗜线虫致病杆菌代谢产物CB6-1的分离、纯化与结构鉴定[J]. 中国抗生素杂志, 2005, 30(9):513-515.
[41] 杨秀芬, 杨怀文, 简恒. 嗜线虫致病杆菌代谢物拮抗大豆疫霉[J]. 大豆科学, 2002(1):52-55.
[42] 李玉华. 嗜线虫杆菌代谢物对马铃薯晚疫病的防治效果[J]. 中国蔬菜, 2003(4):40-45.
[43] 张易, 李佳, 钟娟, 等. 96孔板高通量选育嗜线虫致病杆菌高产帕克素菌株[J]. 中国生物防治学报, 2013, 29(3):437-442.
[44] 杨秀芬, 杨怀文, 简恒, 等. 嗜线虫致病杆菌产生抗生素的培养基及条件[J]. 微生物学通报, 2001, 28(1):12-16.