Bioinformatic Analysis of Insect Kr-h1 Gene

doi:10.16409/j.cnki.2095-039x.2023.00.000

Abstract

Abstract: Kr-h1 (Krüppel homolog 1) is a key molecular target in the regulation of insect metamorphosis and development, and in biological pest control. In this study, insect Kr-h1 gene structure, conserved domain, secondary and tertiary structure, multiple sequences alignment and expression pattern are analyzed, as well as phylogenetic trees are reconstrued. Results indicating that, exon number of Kr-h1 gene containing two exons in most insects, is conserved, while intron in Kr-h1 gene is various in length. Most insects Kr-h1 proteins contain zinc finger structures, which provide the stability of Kr-h1 protein from secondary and tertiary structure evidence. Phylogenetic trees indicate that insect Kr-h1 genes are orthologous among insect species, and genetic relationship of protein fits insect species evolution. However, different motifs of zinc finger structures showed diversities based on phylogenetic tree. Transcription of Pieris rapae data showed close relationship of expression pattern and metamorphosis. Our results enrich knowledge of insect kr-h1gene, providing a new strategy for metamorphosis, development and biological control of P. rapae and Frankliniella occidentalisa.

Key words: Kr-h1, zinc finger, phylogenetic analysis

CLC Number:

WANG Huan, CHEN Yumin, LI Kai, ZHANG Weiwei, DENG Jianling. Bioinformatic Analysis of Insect Kr-h1 Gene[J]. Chinese Journal of Biological Control, 2023, 39(4): 985-994.

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