Biocontrol Effects of Antagonistic Isolate Pc01-6 on Salvia miltiorrhiza Root Rot and Its Genomic Analysis

doi:10.16409/j.cnki.2095-039x.2025.02.015

Abstract

Abstract: In this study, an effective antagonistic isolate, Pc01-6, was isolated and screened from the rhizosphere soil of Salvia miltiorrhiza using the plate confrontation method to explore superior bioncontrol resources against S. miltiorrhiza root rot. The isolate exhibited an inhibition rate of 63.67% to 68.40% against the root rot pathogen, and demonstrated broad-spectrum antagonistic activity against 10 other plant pathogenic fungi. Based on morphological, physiological and biochemical characteristics, as well as 16S rDNA and gyrB sequence analysis, the isolate Pc01-6 was identified as Pseudomonas chlororaphis subsp. Aurantiaca. Pot experiments revealed that the isolate provided a control efficacy of 53.30% to 65.22% against S. miltiorrhiza root rot, while significantly promoting plant growth, with increases of 35.10% and 72.34% in fresh stem and leaf weight and root weights, respectively. Field trials further confirmed its efficiency, showing a control rate of 50.91% against root rot. Whole-genome sequencing analysis indicated that the genome of Pc01-6 is 6,835,850 bp in size, with a GC content of 62.87%, and encodes 6,159 genes. Function gene prediction revealed the presence of gene clusters associated with the biosynthesis of secondary metabolites with antibacterial activity, such as cyclolipopeptides and pyrrolnitrin. In summary, isolate Pc01-6 demonstrates significant biocontrol effects against S. miltiorrhiza root rot and holds promise for practical applications.

Key words: Salvia miltiorrhiza root rot, antagonistic isolate, Pseudomonas chlororaphis subsp. Aurantiaca, whole genome sequencing, secondary metabolite biosynthesis gene cluster

CLC Number:

S476

WANG Fei, LI Xuemeng, YANG Jin, GAO Suxia, GUO Zhichen, QIN Yanhong, WEN Yi, LU Shuhao, ZHAO Ying, SONG Luyang, YANG Jian, WAN Xiufu, LI Shaojian. Biocontrol Effects of Antagonistic Isolate Pc01-6 on Salvia miltiorrhiza Root Rot and Its Genomic Analysis[J]. Chinese Journal of Biological Control, 2025, 41(3): 542-553.

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