Formulation Preparation of Lecanicillium psalliotae ZJLP09 Wettable Powder and Its Control Efficiency against Diaphorina citri

doi:10.16409/j.cnki.2095-039x.2025.01.018

Abstract

Abstract: Lecanicillium psalliotae ZJLP09 is highly pathogenic to Diaphorina citri. To improve practical applying efficiency of this strain in citrus production, the current study screened carriers and auxiliaries in terms of performance and biocompatibility, which determined the following formula of L. psalliotae ZJLP09 wettable powder: 10% ZJLP09 conidia powder, 1% W-2002 as the wetting agent, 1% Dispwet WP-410 as the dispersant, 0.3% ascorbic acid as the UV protector, 0.1% calcium chloride as the spore germination promoter, 0.05% xanthan gum as the adhesive, 87.55% attapulgite clay as the carrier. The quality indicators of the prepared wettable powder confirmed to industry standards for relevant biocontrol agents. Through laboratory toxicity and field control tests, it was determined that the corrected mortality of D. citri treated for 5 days with a preparation with a concentration of 1.0×10⁸ conidia/mL reached 92.5% at climate chamber conditions and 80.55% at field greenhouse conditions. Compared to pure conidia suspension, L. psalliotae ZJLP09 wettable powder has a significantly improved efficiency against D. citri, which supports its use in biocontrol of D. citri in citrus production.

Key words: Lecanicillium psalliotae, wettable powder, Diaphorina citri, control effect

CLC Number:

S476.12

LU Lianming, DU Danchao, SU Guanglang, LIU Shunmin, WU Renchao, QU Simin, AN Baoju, CHEN Guoqing. Formulation Preparation of Lecanicillium psalliotae ZJLP09 Wettable Powder and Its Control Efficiency against Diaphorina citri[J]. Chinese Journal of Biological Control, 2025, 41(4): 796-807.

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