Isolation and Identification of Leclercia adecarboxylata and Its Effect on Promote Plant Growth

doi:10.16409/j.cnki.2095-039x.2025.02.042

Abstract

Abstract: The highly efficient growth-promoting strains were screened and their potential was analyzed to provide excellent strain resources for the research and development of microbial agents. In this study, Salkowski assay was used to select the microorganisms capable to produce indole-3-acetic acid (IAA) in soil and quantitatively screen high-yielding IAA-producing strains. Morphological, physiological and biochemical characteristics, combined with the phylogenetic analysis based on 16S rDNA gene sequences, were employed to identify the strain. The growth-promoting potential of the strains was evaluated based on its capabilities in nitrogen fixation, siderophore production, dissolve phosphorus and potassium releasing. Furthermore, the growth-promoting effect of the strain was detected by pot and field experiments. The results demonstrated that 15 strains with a high capacity for IAA synthesis were obtained, among which ZF523 exhibited the highest IAA production (98.71 μg/mL). and was identified as Leclercia adecarboxylata, furthermore, it has the abilities of nitrogen fixation, siderophore production, phosphate solubilization and potassium releasing. The inoculating the suspension of Leclercia adecarboxylata ZF523 could effectively improve the plant height and root length of cucumber, maize and wheat seedlings. Compared with the water control (CK), the plant height and biomass of Chinese cabbage, cucumber treated with ZF523 increased by 25.98% and 40.96%, 17.01% and 16.12%, respectively. Result of field experiments indicated that the plant height and fresh weight of savoy, Sonchus oleraceus, butterhead lettuce, celery and mini Chinese cabbage were effectively increased by Leclercia adecarboxylata treatment. It suggested that strain ZF523 has the potential to promote plant growth and serves as an excellent broad-spectrum biological resource for growthpromoting bacteria.

Key words: Leclercia adecarboxylata, molecular biological identification, gowth promoting property, field trials

CLC Number:

S476

LIU Yang, WANG Qiao, XIE Xuewen, SHI Yanxia, CHAI Ali, FAN Tengfei, LI Baoju, LI Lei. Isolation and Identification of Leclercia adecarboxylata and Its Effect on Promote Plant Growth[J]. Chinese Journal of Biological Control, 2025, 41(4): 949-958.

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