我国利用真菌防治害虫的历史、进展及现状

doi:10.16409/j.cnki.2095-039x.2015.05.010

摘要/Abstract

摘要： 我国利用虫生真菌防治害虫起步较晚,始于20世纪50年代,迄今只有约60年历史。经过1969年在广东省新会县召开的南方8省(区)利用白僵菌防治松毛虫现场交流会以及2002年“杀蝗绿僵菌油悬浮剂”获农业部农药检定所登记这两个里程碑式的事件,我国不仅建立了世界生产规模最大而且技术先进的真菌杀虫剂产业以及形成以利用球孢白僵菌防治马尾松毛虫和玉米螟为代表的世界最大规模的成功的生物防治项目,而且在虫生真菌的分类学、病理生物学、遗传育种以及生态学等研究领域全面地赶上了国际先进水平,在分子致病机制、基因工程以及基因组学等方面还取得了举世瞩目的研究成果。本文综述了我国利用真菌防治害虫近60年的发展历史,介绍了近年来在细胞核及线粒体变异和菌种退化、分子致病机制、基因工程、比较基因组学、遗传多样性、利用真菌持续控制害虫的生态机制、真菌杀虫剂应用效果的精确评价以及真菌流行病的溯源等领域的研究进展及现状。

关键词: 虫生真菌, 真菌杀虫剂, 生物防治, 白僵菌

Abstract: The application of entomopathogenic fungi against pest insects started as late as the 1950s, with a history of only ca. 60 years. After 2 milestone events, on-the-spot workshop of the application of Beauveria bassiana against pine caterpillars, Xinhui, Guangdong in 1969, and the registration of an acridicidal oil suspension of Metarhizium acridum in 2002, a largest fungal insecticide industry with advanced technology has been established, with the largest application scale in the world, characterized by B. bassiana against pine caterpillars and Asian corn borer as one of the world renowned successful biological control programs, as the overall research has caught up globally advanced level, with world spotlight progress in the fields of molecular pathogenesis, gene engineering and comparative genomics. The present paper reviews the 60 years history of the application of entomopathogenic fungi against pests in China, and describes the research progress and current status in nuclear and mitochondrial variation and strain degeneration, molecular pathogenesis, gene engineering, comparative genomics, genetic diversity, ecological mechanism of sustainable pest control by entomopathogenic fungi, accurate evaluation of field efficacy of fungal insecticides, and tracing of fungal epizootics.

Key words: entomogenous fungi, fungal insecticide, biological control, Beauveria bassiana

中图分类号:

S476.12

李增智. 我国利用真菌防治害虫的历史、进展及现状[J]. 中国生物防治学报, 2015, 31(5): 699-711.

LI Zengzhi. History, Progress and Current Status of the Application of Fungi against Pest Insects in China[J]. journal1, 2015, 31(5): 699-711.

参考文献

[1] 林伯欣. 应用白僵菌防除甘薯象鼻虫初报[J]. 昆虫学报, 1956, 6(4): 539-540.
[2] 徐庆丰. 应用白僵菌防治大豆象食心虫的初步研究[J]. 昆虫学报, 1959, 9(3): 203-215.
[3] 李运帷, 吕昌仁, 陶恒才. 白僵菌的生产和应用[M]. 北京: 中国林业出版社, 1981.
[4] 吕昌仁. 利用白僵菌防治栎褐天社蛾研究简报[J]. 林业科学, 1960, (3): 237.
[5] 林伯欣. 应用荔蝽菌防除荔枝蝽初报[J]. 昆虫知识, 1959, 5(3): 102-104.
[6] 曾省, 尹莘耘. 一种寄生昆虫的穗状菌(Spicaria sp.)研究初报[J]. 植物保护学报, 1962, 1(4): 332-333.
[7] 曾省, 尹莘耘, 赵玉清. 一种虫生真菌–赤色穗状菌Spicaria fumosorosea (Wize) Vassilijevsky的研究[J]. 植物保护学报, 1965, 4(1): 59-68.
[8] 邓庄. 白僵菌生物学特性的研究[J]. 植物学报, 1962, 3(3): 210-232.
[9] Li Z. Beauveria bassiana for pine caterpillar management in the People's Republic of China//Vincent C, M Goettel, G Lazarovits, eds. Biological Control, a Global Perspective[M]. Oxford: CAB International, 2007, 300-310.
[10] 李增智. 虫生真菌在害虫治理中的应用现状[J]. 安徽农学院学报, 1987, 14(2): 59-66.
[11] 黄秀梨, 项伯衡. 白僵菌Beauveria bassiana的几丁质酶[J]. 北京师范大学学报(自然科学版), 1981, (2): 91-94.
[12] 吴正凯, 邓春生. 白僵菌原生质体的形成与再生[J]. 生物防治通报, 1986, 2(4): 186.
[13] 刘健, 陈洪章, 李佐虎. 白僵菌杀虫剂生产工艺研究状况与展望[J]. 中国生物防治, 2003, 19(2): 86-90.
[14] 徐庆丰. 白僵菌安全性及其作为微生物杀虫剂的评价[J]. 生物防治通报, 1991, 7(2): 77-80.
[15] 潘务耀, 陈沐荣, 文志忠, 等. 白僵菌乳剂的研制[J]. 森林病虫通讯, 1985, (2): 5-9.
[16] 潘务耀, 殷凤鸣. 广东省白僵菌粉剂的企业标准[J]. 林业科技通讯, 1989, (3): 4-6.
[17] 胡锦江, 樊美珍, 文建雷, 等. 球孢白僵菌对马尾松毛虫的毒力与有关生化指标的关系. Ⅲ, 不同世代白僵菌胞外蛋白酶的毒力[J]. 西北林学院学报, 1993, 8(4): 36-40.
[18] 陆庆光, 邓春生, 张爱文, 等. 四种不同绿僵菌菌株对东亚飞蝗毒力的初步观察[J]. 生物防治通报, 1993, 10(4): 187.
[19] 张爱文, 刘维真, 邓春生, 等. 白僵菌制剂不同剂型防治玉米螟的研究[J]. 生物防治通报, 1990, 6(3): 118-120.
[20] 王成树, 李农昌, 李增智. 真菌杀虫剂标准化问题的探讨[J]. 中国生物防治, 1998, 14(3): 134-138.
[21] 李增智, 韩宝瑜, 樊美珍, 等. 应用白僵菌防治马尾松毛虫的策略及其生物多样性基础的研究[J]. 应用生态学报, 1998, 9(3): 503-510.
[22] ICAMA(中国农药信息网). 农药电子手册, http://www.agrolex.com.cn. 2008.
[23] Faria M R, Wraight S P. Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types[J]. Biological Control, 2007, 43(3): 237-256.
[24] 宋玉双, 黄北英. 我国白僵菌市场分析及对策[J]. 世界林业研究, 2006, 19(6): 53-57.
[25] 陈培荣, 王滨, 姚洪章, 等. 纳米ZnO对白僵菌孢子的紫外保护及与孢子的生物相容性[J]. 高等学校化学学报, 2010, 31(12): 2322-2328.
[26] 徐延平, 仇飞, 黄勃, 等. 一株小菜蛾高致病性球孢白僵菌菌株的筛选及其可湿性粉剂的田间药效试验[J]. 中国生物防治学报, 2013, 29(3): 376-383.
[27] 展茂魁, 何玲敏, 陈名, 等. 防治假眼小绿叶蝉的虫生真菌高毒菌株的筛选及田间防治效果[J]. 中国生物防治学报, 2012, 28(1): 41-46.
[28] 赵军, 杨震元, 张胜利, 等. 几种微胶囊适宜加工材料与球孢白僵菌分生孢子的相容性[J]. 中国生物防治, 2010, 26(1): 40-46.
[29] Feng M, Chen C, Ying S. Trials of Beauveria bassiana, Paecilomyces fumosoroseus and imidacloprid for management of Trialeurodes vaporariorum (Homoptera: Aleyrodidae) on greenhouse grown lettuce[J]. Biocontrol Science and Technology, 2004, 14(6): 531-544.
[30] Liu Y, Liu J, Ying S, et al. A fungal insecticide engineered for fast per os killing of caterpillars has high field[J]. Applied and Environmental Microbiology, 2013, 79(20): 6452-6458.
[31] Shi W, Zhang L, Feng M. Field trials of four formulations of Beauveria bassiana and Metarhizium anisoplae for control of cotton spider mites (Acari: Tetranychidae) in the Tarim Basin of China[J]. Biological Control, 2008, 45(1): 48-55.
[32] 应盛华, 冯明光. 球孢白僵菌分生孢子乳悬剂的配方筛选[J]. 植物保护学报, 2001, 28(4): 345-351.
[33] Peng G, Wang Z, Yin Y, et al. Field trials of Metarhizium anisopliae var. acridum (Ascomycota: Hypocreales) against oriental migratory locus, Locusta migratoria manilensis (Meyen) in northern China[J]. Crop Protection, 2008, 27(9): 1244-1250.
[34] 刘淼, 武觐文. 粉拟青霉异核性研究[J]. 真菌学报, 1992, 11(3): 234-242.
[35] 王成树, 王四宝, 李增智, 等. 球孢白僵菌种群野生菌株异核现象的分子验证[J]. 菌物系统, 2001, 20(4): 475-481.
[36] Wang C S, Li Z Z, Butt T M. Molecular studies of co-formulated strains of the entomopathogenic fungus, Beauveria bassiana[J]. Journal of Invertebrate Pathology, 2002, 80(1): 29-34.
[37] Tang X, Fan M, Li Z. Strain variation of Beauveria bassiana in subculturing[J]. Mycosystema, 1995-1996, 8-9(1): 137-151.
[38] 唐晓庆, 李增智, 樊美珍. 球孢白僵菌继代培养中菌落局变现象及环境因素的研究[J]. 真菌学报, 1996, 15(3): 188-196.
[39] Xu J, Huang B, Qin C, et al. Sequence and phylogenetic analysis of Beauveria bassiana with mitochondrial genome[J]. Mycosystema, 2009, 28(5): 718-723.
[40] 徐金柱, 黄勃, 李增智. 球孢白僵菌单孢子分离株在继代培养过程中菌落局变的遗传分析[J]. 菌物学报, 2010, 29(2): 285-293.
[41] 汪黎明, 陈雪, 徐延平, 等. 一株球孢白僵菌转基因工程菌在继代培养中的变异[J]. 菌物学报, 2011, 30(5): 738-743.
[42] Charnley A K, St Leger R J. The role of cuticle–degrading enzymes in fungal pathogenesis in insects//Cole E T, Hoch H C, eds. Fungal Spore Disease in Initiation in Plants and Animals[M]. New York: Plenum Press, 1991, 267-287.
[43] St Leger R J, Frank D C, Roberts D W, et al. Molecular cloning and regulatory analysis of the cuticle–degrading protease structural gene from the entomopathogenic fungus Metarhizium anisopliae[J]. European Journal of Biochemistry, 1992, 204(3): 991-1001.
[44] Wang C, Feng M. Advances in fundamental and applied studies in China of fungal biocontrol agents for use against arthropod pests[J]. Biological Control, 2014, 68(1): 129-135.
[45] Gao Q, Jin K, Ying S, et al. Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum[J]. PLoS Genetics, 2011, 7(1): e1001264.
[46] Zheng P, Xia Y, Xiao G, et al. Genome sequence of the insect pathogenic fungus Cordyceps militaris, a valued traditional Chinese medicine[J]. Genome Biology, 2011, 12(11): R116.
[47] Xiao G, Ying S, Zheng P, et al. Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana[J]. Scientific Reports, 2012, 2: 483.
[48] Hu X, Zhang Y, Xiao G, et al. Genome survey uncovers the secrets of sex and lifestyle in caterpillar fungus[J]. Chinese Science Bulletin, 2013, 58(23): 2846-2854.
[49] Hu X, Xiao G, Zheng P, et al. Trajectory and genomic determinants of fungal-pathogen speciation and host adaptation[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(47): 16796-16801.
[50] Wang C, St Leger R J. The MAD1 adhesion of Metarhizium anisopliae links adhesion with blastospore production and virulence to insects, and the MAD2 adhesion enables attachment to plants[J]. Eukaryotic Cell, 2007, 6(5): 808-816.
[51] Li J, Ying S, Shan L, et al. A new non–hydrophobic cell wall protein (CWP10) of Metarhizium anisopliae enhances conidial hydrophobicity when expressed in Beauveria bassiana[J]. Applied Microbiology and Biotechnology, 2010, 85(4): 975-984.
[52] Zhang S, Xia Y, Kim B, et al. Two hydrophobins are involved in fungal spore coat rodlet layer assembly and each play distinct roles in surface interactions, development and pathogenesis in the entomopathogenic fungus, Beauveria bassiana[J]. Molecular Microbiology, 2011, 80(6): 811-826.
[53] Wang C, St Leger R J. The Metarhizium anisopliae perilipin homolog MPL1 regulates lipid metabolism, appressorial turgor pressure, and virulence[J]. Journal of Biological Chemistry, 2007, 282(29): 21110-21115.
[54] Duan Z, Chen Y, Huang W, et al. Linkage of autophagy to fungal development, lipid storage and virulence in Metarhizium robertsii[J]. Autophagy, 2013, 9(4): 538-549.
[55] Gao Q, Shang Y, Huang W, et al. Glycerol–3–phosphate acyltransferase contributes to triacylglycerol biosynthesis, lipid droplet formation and host invasion in Metarhizium robertsii[J]. Applied and Environmental Microbiology, 2013, 79(24): 7646-7653.
[56] Fang W, Leng B, Xiao Y, et al. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence[J]. Applied and Environmental Microbiology, 2005, 71(1): 363-370.
[57] Fang W, Feng J, Fan Y, et al. Expressing a fusion protein with protease and chitinase activities increases the virulence of the insect pathogen Beauveria bassiana[J]. Journal of Invertebrate Pathology, 2009, 102(2): 155-159.
[58] Wang C, Feng M. Advances in fundamental and applied studies in China of fungal biocontrol agents for use against arthropod pests[J]. Biological Control, 2014, 68(1): 129-135.
[59] Huang W, Shang Y, Chen P, et al. Basic leucine zipper (bZIP) domain transcription factor MBZ1 regulates cell wall integrity, spore adherence, and virulence in Metarhizium robertsii[J]. Journal of Biological Chemistry, 2015, 290(13): 8218-8231.
[60] Huang W, Shang Y, Chen P, et al. MrpacC regulates sporulation, insect cuticle penetration and immune evasion in Metarhizium robertsii[J]. Environmental Microbiology, 2015, 17(4): 994-1008.
[61] Wang C, Duan Z, St Leger R J. The MOS1 osmosensor of Metarhizium anisopliae is required for adaptation to insect host hemolymph[J]. Eukaryotic Cell, 2008, 7(2): 302-309.
[62] Zhang Y, Zhao J, Fang W, et al. Mitogen–activated protein kinase hog1 in the entomopathogenic fungus Beauveria bassiana regulates environmental stress responses and virulence to insects[J]. Applied and Environmental Microbiology, 2009, 75(11): 3787-3795.
[63] Duan Z, Shang Y, Gao Q, et al. A phosphoketolase Mpk1 of bacterial origin is adaptively required for full virulence in the insect–pathogenic fungus Metarhizium anisopliae[J]. Environmental Microbiology, 2009, 11(9): 2351-2360.
[64] Jin K, Peng G, Liu Y, et al. The acid trehalase, ATM1, contributes to the in vivo growth and virulence of the entomopathogenic fungus, Metarhizium acridum[J]. Fungal Genetics and Biology, 2015, 77(1): 61-67.
[65] Shang Y, Chen P, Chen Y, et al. MrSkn7 controls sporulation, cell wall integrity, autolysis, and virulence in Metarhizium robertsii[J]. Eukaryotic Cell, 2015, 14(4): 396-405.
[66] Wang C, St Leger R J. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(17): 6647-6652.
[67] Wang B, Kang Q, Lu Y, et al. Unveiling the biosynthetic puzzle of destruxins in Metarhizium species[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(4): 1287-1292.
[68] Chen Y, Duan Z, Chen P, et al. The Bax inhibitor MrBI–1 regulates heat tolerance, apoptotic–like cell death, and virulence in Metarhizium robertsii[J]. Scientific Reports, 2015, 5: 10625.
[69] Wang C, St Leger R J. A scorpion neurotoxin increases the potency of a fungal insecticide[J]. Nature Biotechnology, 2007, 25(12): 1455-1456.
[70] Lu D, Pava–Ripoll M, Li Z, et al. Insecticidal evaluation of Beauveria bassiana engineered to express a scorpion neurotoxin and a cuticle degrading protease[J]. Applied Microbiology and Biotechnology, 2008, 81(3): 515-522.
[71] 郝蕾蕾, 陈珊珊, 谢翎, 等. 几丁质酶基因在球孢白僵菌中的超表达提高其对马尾松毛虫的毒力[J]. 中国生物防治, 2010, 26(3): 267-273.
[72] Fang W, Leng B, Xiao Y. Cloning of Beauveria bassiana chitinase gene Bbchit1 and it s application to improve fungal strain virulence[J]. Applied and Environmental Microbiolgy, 2005, 71(1): 363-370.
[73] Zheng P, Xia Y, Xiao G, et al. Genome sequence of the insect pathogenic fungus Cordyceps militaris, a valued traditional chinese medicine[J]. Genome Biology, 2011, 12: R116.
[74] Xiao H, Xiao G, Zheng P, et al. Trajectory and genomic determinants of fungal–pathogen speciation and host adaptation[J]. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(47): 16796-16801.
[75] Xiang L, Li Y, Zhu Y, et al. Transcriptome analysis of the Ophiocordyceps sinensis fruiting body reveals putative genes involved in fruiting body development and cordycepin biosynthesis[J]. Genomics, 2014, 103(1): 154-159.
[76] Liu Z, Lin S, Baker P J, et al. Transcriptome sequencing and analysis of the entomopathogenic fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis[J]. BMC Genomics, 2015, 16(1): 106.
[77] 张引芳. RFLP 在鳞翅目蛹虫草菌鉴别中的应用[J]. 食用菌学报, 1994, 1(2): 21-24.
[78] 陈永久, 宿兵, 王文, 等. 用RAPD标记分析冬虫夏草(Cordyceps sinensis [Berk.] Sacc.)个体的遗传特性//中国虫生真菌研究与应用第四卷[M]. 北京: 中国农业科技出版社, 1997.
[79] Wang C, Shah F, Patel N, et al. Molecular investigation on strain genetic relatedness and population structure of Beauveria bassiana[J]. Environmental Microbiology, 2003, 5(10): 908-925.
[80] Wang C, Li Z, Typas M, et al. Nuclear large subunit rDNA group I intron distribution in a population of Beauveria bassiana strains: phylogenetic implications[J]. Mycological Research, 2003, 107(10): 1189-1200.
[81] Rehner S A, Buckley E P. A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs[J]. Mycologia, 2005, 97(1): 84-98.
[82] Rehner S A, Buckley E P. Isolation and characterization of microsatellite loci from the entomopathogenic fungus Beauveria bassiana (Ascomycota: Hypocreales)[J]. Molecular Ecology Notes, 2003, 3: 409-411.
[83] Wang S, Miao X, Zhao W, et al. Genetic diversity and population structure among strains of entomopathogenic fungus, Beauveria bassiana, as revealed by inter–simple sequence repeat (ISSR)[J]. Mycological Research, 2005, 109(12): 1364-1372.
[84] 苏宇, 农向群, 张泽华. 绿僵菌M189菌株特异性SCAR标记的建立及田间监测应用[J]. 菌物学报, 2012, 31(3): 366-373.
[85] 王成树, 黄勃, 王四宝, 等. 球孢白僵菌培养特征多样性与寄生及地理来源的相关性分析[J]. 生物多样性, 2002, 10(2): 196-201.
[86] 杜广祖, 王晶晶, 陈斌, 等. 云南球孢白僵菌的遗传多样性研究[J]. 云南农业大学学报(自然科学版), 2012, 27(3): 327-333.
[87] 吕思行, 刘吉平, 汤历, 等. 两广地区家蚕白僵菌的SSRs遗传多样性[J]. 昆虫学报, 2012, 55(10): 1142-1148.
[88] 陈雪娇, 李云飞, 孙娟娟. 利用ISSR标记分析红脂大小蠹微生境中球孢白僵菌遗传多样性[J]. 菌物学报, 2014, 33(5): 1015-1024.
[89] 丁德贵, 李增智, 樊美珍, 等. 球孢白僵菌种群在松林中的寄主转移及遗传多样性对松毛虫持续控制的影响[J]. 应用生态学报, 2004, 15(12): 2315-2320.
[90] 王滨. 白僵菌持续控制马尾松毛虫的生物多样性和生态学基础[D]. 杭州: 浙江大学, 2003.
[91] 赵学球, 蔡守平, 王四宝, 等. 球孢白僵菌释放后在森林系统中定殖及持续控虫作用的证据[J]. 菌物学报, 2007, 26(1): 84-88.
[92] 赵文琴, 樊美珍, 丁德贵, 等. 释放菌株与宣城放菌区流行菌株营养亲和群的研究[J]. 安徽农业大学学报, 2008, 35(1): 33-37.
[93] 蒲顺昌, 秦丽, 陈名君, 等. 马尾松林中球孢白僵菌寄主转移和专化性的SSR 标记分析[J]. 菌物学报, 2013, 32(4): 698-709.
[94] Chen M, Liu Y, Li Z, et al. Comparative study on genetic diversity of Beauveria bassiana in different forest ecosystems[J]. Plant Diseases and Pests, 2010, 1(6): 68-72.
[95] 栾丰刚. 引起昆虫自然流行病的昆虫病原真菌种群遗传结构研究[D]. 合肥: 安徽农业大学, 2011.
[96] 张胜利. 引起昆虫地方病的一些昆虫病原真菌种群遗传结构的研究[D]. 合肥: 安徽农业大学, 2012.
[97] 胡晓磊, 何玲敏, 陈雪, 等. 中国南方球孢白僵菌的遗传多样性和种群遗传结构[J]. 中国生物防治学报, 2013, 29(1): 31-41.
[98] 何玲敏, 胡晓磊, 陈雪, 等. 中国北方球孢白僵菌的遗传多样性和种群遗传结构[J]. 应用生态学报, 2012, 23(11): 3087-3095.
[99] Chen X, Huang C, He L, et al. Molecular tracing of white muscardine in the silkworm, Bombyx mori (Linn.) II. Silkworm white muscardine is not caused by artificial release or natural epizootic of Beauveria bassiana in China[J]. Journal of Invertebrate Pathology, 2015, 125(1): 16-22.
[100] 陈雪. 球孢白僵菌种群遗传结构及家蚕白僵病分子溯源研究[D]. 合肥: 安徽农业大学, 2014.
[101] 胡本进. 球孢白僵菌在亚洲玉米螟中的流行病学研究[D]. 合肥: 安徽农业大学, 2014.
[102] 周娜, 陈名君, 黄勃, 等. 引起蜘蛛流行病的紫色野村菌种群异质性[J]. 菌物学报, 2010, 29(1): 37-43.
[103] 李增智, 韩宝瑜, 樊美珍, 等. 应用球孢白僵菌防治马尾松毛虫的策略及其生物多样性基础的研究[J]. 应用生态学报, 1998, 9(3): 503-510.
[104] 陈名君, 刘玉军, 周娜, 等. 马尾松纯林虫生真菌群落组成及优势种生态位研究[J]. 菌物学报, 2009, 28(4): 521-527.
[105] 陈名君, 黄勃, 李增智. 三种森林生态系统昆虫病原真菌优势种生态位比较[J]. 应用生态学报, 2011, 22(5): 1275-1279.
[106] 陈名君, 黄勃, 刘玉军, 等. 马尾松纯林中球孢白僵菌种群的遗传异质性[J]. 应用生态学报, 2010, 21(10): 2627-2634.
[107] 丁德贵, 樊美珍, 孟艳琼, 等. 应用球孢白僵菌菌条持续控制松褐天牛效果的分子评价[J]. 应用生态学报, 2007, 18(3): 564-568.
[108] Luan F, Zhang S, Cai Y, et al. Identification of the molecular origin and development of a panzootic caused by Beauveria bassiana in praying mantis populations in eastern China[J]. Journal of Invertebrate Pathology, 2011, 108(2): 98-105.
[109] Luan F, Zhang S, Wang B, et al. Genetic diversity of the fungal pathogen Metarhizium spp., causing epizootics in Chinese burrower bugs in the Jingting Mountains, eastern China[J]. Molecular Biology Reports, 2012, 40(1): 515-523.
[110] 金伟. 家蚕病理学[M]. 北京: 中国农业出版社, 2000, 36-38, 143-145.
[111] 朱方容. 广西部分地区桑蚕僵病流行的原因及防控对策[J]. 广西蚕业, 2008, 45(3): 29-32.
[112] 石美宁, 朱方容. 广西林用白僵菌生物农药对家蚕的致病性试验[J]. 广西农业科学, 2010, 41(10): 1131-1133.
[113] 李佳丽, 蔡悦, 栾丰刚, 等. 皖南与皖西南蚕区球孢白僵菌种群遗传结构的ISSR分析[J]. 应用生态学报, 2010, 21(12): 3239-3247.
[114] 李佳丽, 栾丰刚, 李增智. 安徽西南部家蚕白僵病病源及传播途径追踪[J]. 中国农业科学, 2011, 44(1): 143-152.
[115] 黄翠, 王品, 陈雪, 等. 球孢白僵菌松毛虫分离株对家蚕的致病力[J]. 中国生物防治学报, 2014, 29(2): 199-209.