茶卡盐湖中耐盐抗真菌芽孢杆菌的分离和鉴定

doi:10.16409/j.cnki.2095-039x.2016.04.004

中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (4): 440-448.DOI: 10.16409/j.cnki.2095-039x.2016.04.004

茶卡盐湖中耐盐抗真菌芽孢杆菌的分离和鉴定

邵高祥^1,2, 束长龙², 耿丽丽², 高继国¹, 刘贵明³, 张达¹, 张杰²

1. 东北农业大学生命科学学院, 哈尔滨 150030;
2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;
3. 中国科学院北京基因组研究所, 北京 100101

收稿日期:2016-02-01 出版日期:2016-08-08 发布日期:2016-07-29
通讯作者: 张达, 张杰
作者简介:邵高祥(1988?),男,硕士研究生,E-mail:shaogaoxiang_66@126.com
基金资助:
中国农业科学院科技创新工程

Isolation and Identification of Antifungal Bacillus with Salt-tolerant in Caka Salt Lake

SHAO Gaoxiang^1,2, SHU Changlong², GENG Lili², GAO Jiguo¹, LIU Guiming³, ZHANG Da¹, ZHANG Jie²

1. College of Life Science, Northeast Agricultural University, Harbin 150030, China;
2. State Key Laboratory of Biology for Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Beijing Institute of Genome Research, Chinese Academy of Sciences, Beijing 100101, China

Received:2016-02-01 Online:2016-08-08 Published:2016-07-29

摘要/Abstract

摘要： 本试验从茶卡盐湖水及淤泥中分离得到了51株细菌，通过16S rDNA及gyrB基因测序分析初步确定了菌株的分类地位，并测定了各菌株对苹果根腐病菌Fusarium spp.、辣椒疫霉病菌Phytophthora capsici等13株病原菌的抑菌谱及对NaCl的耐受性。共筛选获得13株对13种真菌病害均有抑菌作用的菌株，13株广谱生防菌株在NaCl浓度为10%的LB培养基上都能正常生长，其中菌株10和27在NaCl浓度为15%的LB培养基中的生长速度显著高于生防菌B916。盐湖环境为耐盐生防菌的筛选提供了丰富的资源，本研究获得的耐盐广谱生防菌对盐碱地真菌病害的防治提供了菌株资源。

关键词: 耐盐, 生防菌, 芽孢杆菌, 真菌病害

Abstract: A total of 51 bacterial strains were isolated from water and silt of a salt lake. Taxonomic status of these strains were primarily identified by 16S rDNA and gyrB gene sequences analysis. Meanwhile the tolerance to NaCl and the antimicrobial spectra against 13 fungal pathogens, including Fusarium spp. and Phytophthora capsici, were tested. A total of 13 bacterial strains showed antimicrobial activity against 13 pathogen fungi which could also grow normally in the LB medium supplemented with 10% NaCl. Among these strains, the growth rate of No.10 and No.27 strains was significantly higher than that of a biocontrol bacterium B916 in the LB medium supplemented with 15% NaCl. The salt lake provides abundant resources for the screening of biocontrol bacteria with salt-tolerant. The biocontrol bacteria with the broad antibacterial spectra and salt-tolerant obtained in this study provided strain resources for control fungal diseases in the saline land.

Key words: salt-tolerant, biocontrol bacterium, Bacillus, pathogenic fungi

中图分类号:

S476

邵高祥, 束长龙, 耿丽丽, 高继国, 刘贵明, 张达, 张杰. 茶卡盐湖中耐盐抗真菌芽孢杆菌的分离和鉴定[J]. 中国生物防治学报, 2016, 32(4): 440-448.

SHAO Gaoxiang, SHU Changlong, GENG Lili, GAO Jiguo, LIU Guiming, ZHANG Da, ZHANG Jie. Isolation and Identification of Antifungal Bacillus with Salt-tolerant in Caka Salt Lake[J]. journal1, 2016, 32(4): 440-448.

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茶卡盐湖中耐盐抗真菌芽孢杆菌的分离和鉴定

Isolation and Identification of Antifungal Bacillus with Salt-tolerant in Caka Salt Lake

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