植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果

doi:10.16409/j.cnki.2095-039x.2017.03.011

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (2/3): 371-377.DOI: 10.16409/j.cnki.2095-039x.2017.03.011

植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果

张猛¹, 王琼¹, 冯发运¹, 袁建军², 余向阳¹

1. 江苏省农业科学院农产品质量安全与营养研究所/江苏省食品质量安全重点实验室/省部共建国家重点实验室培育基地/农业部农产品质量安全控制技术与标准重点实验室, 南京 210014;
2. 泉州师范学院海洋与食品学院, 泉州 362000

收稿日期:2017-01-04 出版日期:2017-06-08 发布日期:2017-04-08
通讯作者: 余向阳,博士,研究员,E-mail:yu981190@hotmail.com;袁建军,硕士,教授,E-mail:yuanjianjun2005@qztc.edu.cn
作者简介:张猛,博士,副研究员,E-mail:z320m320@163.com;王琼,硕士,研究实习员,E-mail:redflower1100@hotmail.com
基金资助:
江苏省农业科技自主创新基金项目（CX-15-1035）；国家自然科学基金（31401779）；江苏省自然科学基金（BK20140745）；泉州市科技计划项目（2013Z39）

Isolation and Identification of Plant Endophyte Bacterial Bacillus tequilens and Its Control Effect against Watermelon Fusarium Wilt

ZHANG Meng¹, WANG Qiong¹, FENG Fayun¹, YUAN Jianjun², YU Xiangyang¹

1. Key Laboratory of Food Quality and Safety of Jiangsu Province/State Key Laboratory Breeding Base/Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, Ministry of Agriculture/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China

Received:2017-01-04 Online:2017-06-08 Published:2017-04-08

摘要/Abstract

摘要： 为了筛选新的生防菌菌种资源，采用稀释涂布平板法从番茄植株中分离得到一株内生细菌wm031，采用平板对峙法研究其对病原菌的抑制效果，结合菌体特征、16S rDNA序列分析及生理生化指标，对其进行初步鉴定，明确分类地位，并采用温室栽培试验对其进行抗病效果初探。结果表明，该菌株对西瓜枯萎病菌具有显著拮抗作用，同时对油菜菌核病菌、小麦根腐病菌、小麦全蚀病菌、辣椒疫霉病菌、大蒜菌核病菌、水稻恶苗病菌、葡萄炭疽病菌和番茄枯萎病菌均具有广谱抗性。根据菌体特征、16S rDNA序列分析及生理生化指标的结果，初步鉴定该菌为特基拉芽胞杆菌Bacillus tequilens。该菌株能够在西瓜和水稻植株中稳定定殖。温室栽培试验表明，菌株wm031对西瓜枯萎病具有显著的防治效果74.6%，高出百菌清的防治效果14.1%，说明该菌株具有田间应用开发的潜力。

关键词: 特基拉芽胞杆菌, 分离, 生防菌, 西瓜枯萎病

Abstract: For the purposes of selecting new biocontrol agents, bacteria wm031 was isolated from the tomato plants using the dilution plate method and its antifungal activity was tested by the dual plate assay. The strain wm031 was identified based on morphological characteristics, 16S rDNA sequence analysis, physiological and biochemical characteristics, and its antagonistic against plant diseases was investigated by greenhouse experiments. The results indicated that the strain wm031 exhibited broad-spectrum antagonism against the tested fungi, including Fusarium oxyporum f. sp. niveum, Sclerotinia sclerotiorum in oilseed rape, Bipolaris sorokinianum, Gaeumannomyces graminis var. tritici Walker, Phytophthora capsici, Sclerotinia allii Saw., Fusarium moniliforme Sheld, Colletotrichum gloeosporioides, and Fusarium oxysporum f. sp. lycopersici. The strain wm031 was identified as Bacillus tequilens, and it could stably colonize in watermelon and rice plants. Strain wm031 demonstrated good control effects on watermelon Fusarium wilt caused by Fusarium oxyporum f. sp. niveum, with the efficacy of 74.6%, higher than that of chlorothalonil by 14.1%. Thus, the strain wm031 is a potential biocontrol agent to be applied in the field.

Key words: Bacillus tequilens, isolation, biocontrol agents, Fusarium wilt of watermelon

中图分类号:

S476

张猛, 王琼, 冯发运, 袁建军, 余向阳. 植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果[J]. 中国生物防治学报, 2017, 33(2/3): 371-377.

ZHANG Meng, WANG Qiong, FENG Fayun, YUAN Jianjun, YU Xiangyang. Isolation and Identification of Plant Endophyte Bacterial Bacillus tequilens and Its Control Effect against Watermelon Fusarium Wilt[J]. journal1, 2017, 33(2/3): 371-377.

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植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果

Isolation and Identification of Plant Endophyte Bacterial Bacillus tequilens and Its Control Effect against Watermelon Fusarium Wilt

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