Cry8E亚致死浓度对马铃薯甲虫解毒酶和保护酶的影响

doi:10.16409/j.cnki.2095-039x.2016.06.018

中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (6): 794-799.DOI: 10.16409/j.cnki.2095-039x.2016.06.018

Cry8E亚致死浓度对马铃薯甲虫解毒酶和保护酶的影响

任羽¹, 郭文超^2,3, 岳明翠⁴, 阿布都热合曼·吐尔逊¹

1. 喀什大学生命与地理科学学院/叶尔羌绿洲生态多样性与生物资源研究重点实验室, 喀什 844000;
2. 新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室, 乌鲁木齐 830091;
3. 新疆农业科学院微生物研究所, 乌鲁木齐 830091;
4. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193

收稿日期:2015-12-07 出版日期:2016-12-08 发布日期:2016-12-07
通讯作者: 任羽
作者简介:任羽(1979-),博士,副教授,硕士生导师,E-mail:renyu1020@aliyun.com
基金资助:
新疆维吾尔自治区教育厅高校科研计划（XJEDU2013S25）；新疆维吾尔自治区科技创新团队（201475002）；喀什大学高层次人才科研启动经费（GCC14ZK-001）

Influence of Cry8E Sublethal Doses on Activities of Detoxification Enzymes and Protective Enzyme of the Potato Beetle Larvae

REN Yu¹, GUO Wenchao^2,3, YUE Mingcui⁴, ABDUL Reheman·Tursun¹

1. College of Life and Geographical Science, Kashi University/Key Laboratory of Ecology and Biological Resources in Yarkand Oasis, Kashi 844000, China;
2. Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Intergraded Management of Harmful Crop Vermin of China North-western Oasis, Ministry of Agriculture, Urumqi 830091, China;
3. Institute of Microbiology, Urumqi 830091, China;
4. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2015-12-07 Online:2016-12-08 Published:2016-12-07

摘要/Abstract

摘要： 苏云金芽胞杆菌Cry8E杀虫晶体蛋白对马铃薯甲虫具有中等毒性，用亚致死剂量9和7 μg/mL的Cry8E分别饲喂2、3和4龄的马铃薯甲虫幼虫，然后测定羧酸酯酶（CarE）、乙酰胆碱酯酶（AChE）、谷胱甘肽-S-转移酶（GST）和过氧化氢酶（CAT）的酶活性。β-羧酸酯酶的活性随Cry8E浓度的升高明显提高，随龄期增长活性降低，酶活性在浓度间和龄期间差异都极显著。谷胱甘肽-S-转移酶活性下降，2龄幼虫体内的乙酰胆碱酯酶的活性明显升高，并且随龄期变化差异显著（P<0.01）。3龄幼虫体内的CAT酶活性随Cry8E浓度的增加而显著升高（P<0.01）。本研究阐明了Cry8E对马铃薯甲虫解毒酶和保护酶活性的影响，为理论研究奠定基础。

关键词: 苏云金芽胞杆菌, 马铃薯甲虫, 羧酸酯酶, 乙酰胆碱酯酶, 谷胱甘肽-S-转移酶, 过氧化氢酶

Abstract: In this paper, impact of Bacillus thuringiensis insecticidal crystal protein Cry8E to the activities of detoxification enzymes such as CarE, AChE, GST and of the catalase (CAT) in the midgut of the potato beetle larvae was investigated. The 2nd, 3rd and 4th instar larvae of the beetle were fed with the Cry8E insecticidal crystal protein at concentrations of 9 and 7 μg/mL. Then the activities of CarE, AChE, GST and CAT in the larvae were measured by the spectrophotometry. The LC₅₀ was 11.3 μg/mL against the 1st instar larvae of the beetle. CarE activity increased significantly in treated insects than in the control and differed significantly between the two concentration treatments. And CarE activity of the 2nd instar larvae was different from that of the 4th instar larvae. The AChE activity in the 2nd instar larvae was higher in the treated insects than in the control, but was not different between the two concentration treatments. And AChE activity of the 2nd instar larvae differed significantly from that of the 3rd and 4th instars. GST activity was significantly lower in the treatments than in the control and differed significantly between the 2nd and the 3rd, 4th instar larvae. CAT activity increased and then decreased, and differed between the treatments and the control. And CAT activity also differed between the 3rd and 2nd, 4th instar larvae. These results show that the potato beetle can adapt to the impacts caused by the Cry8E insecticidal crystal proteins through changing activities of the detoxification enzymes and protective enzymes, which is influenced by the larval stage and protein concentration.

Key words: Bacillus thuringiensis, potato colorado beetle, caboxylesterase (CarE), acetylcholinesterase (AChE), glutathione-S-transferase (GST), catalase (CAT)

中图分类号:

S476.11

任羽, 郭文超, 岳明翠, 阿布都热合曼·吐尔逊. Cry8E亚致死浓度对马铃薯甲虫解毒酶和保护酶的影响[J]. 中国生物防治学报, 2016, 32(6): 794-799.

REN Yu, GUO Wenchao, YUE Mingcui, ABDUL Reheman·Tursun. Influence of Cry8E Sublethal Doses on Activities of Detoxification Enzymes and Protective Enzyme of the Potato Beetle Larvae[J]. journal1, 2016, 32(6): 794-799.

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Cry8E亚致死浓度对马铃薯甲虫解毒酶和保护酶的影响

Influence of Cry8E Sublethal Doses on Activities of Detoxification Enzymes and Protective Enzyme of the Potato Beetle Larvae

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