苏云金芽胞杆菌杀线虫活性因子分析及杀虫机制研究进展

doi:10.16409/j.cnki.2095-039x.2017.05.019

摘要/Abstract

摘要： 苏云金芽胞杆菌Bacillus thuringiensis（Bt），是一种重要的昆虫病原细菌，对多种农业害虫包括鳞翅目、鞘翅目、双翅目、膜翅目和线虫等具有高特异杀虫活性，是迄今应用最广泛的微生物杀虫剂。本文总结了杀线虫Bt菌株的活性因子种类以及不同类的活性物质杀线虫机制的分析，能够为生物杀线剂的研究与创制提供理论基础。

关键词: 苏云金芽胞杆菌, 杀线虫活性因子, 杀线虫机制

Abstract: Bacillus thuringiensis abbreviated as Bt, is a kind of important insect pathogenic bacteria with high specific insecticidal activity against various agricultural pests, including Lepidoptera, Coleoptera, Diptera, Hymenoptera and nematode. Bt is by far the most widely used microbial insecticide. In this paper, we summarized the types of active factors and the nematicidal mechanism, which could provide the theoretical basis for the research and development of the bio-nematicides.

Key words: Bacillus thuringiensis, nematicidal factors, nematicidal mechanism

中图分类号:

S476.1

刘晓艳, 闵勇, 黄大野, 方伟, 王开梅, 杨自文. 苏云金芽胞杆菌杀线虫活性因子分析及杀虫机制研究进展[J]. 中国生物防治学报, 2017, 33(5): 713-718.

LIU Xiaoyan, MIN Yong, HUANG Daye, FANG Wei, WANG Kaimei, YANG Ziwen. Research Advances on the Nematicidal Factors and Virulence Mechanisms of Bacillus thuringiensis[J]. journal1, 2017, 33(5): 713-718.

参考文献

[1] Schnepf E, Crickmore N, Van Rie J, et al. Bacillus thuringiensis and its pesticidal crystal proteins[J]. Microbiology and Molecular Biology Reviews, 1998, 62(3):775-806.
[2] Salehi J G, Abad A P, Seifinejad A, et al. Distribution and diversity of dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran[J]. Journal of Industrial Microbiology and Biotechnology, 2008, 35(2):83-94.
[3] Salehi J G, Seifinejad A, Saeedizadeh A, et al. Molecular detection of nematicidal crystalliferous Bacillus thuringiensis strains of Iran and evaluation of their toxicity on free-living and plant-parasitic nematodes[J]. Canadian Journal of Microbiology, 2008, 54(10):812-822.
[4] Jouzani G S, Valijanian E, Sharafi R. Bacillus thuringiensis:a successful insecticide with new environmental features and tidings[J]. Applied Microbiology and Biotechnology, 2017, 101(7):2691-2711.
[5] Ciordia H, Bizzell W E. The effects of various constant temperatures on the development of the free living-stages of some nematode parasites of cattle[J]. Journal of Parasitology, 1963, 49:60-63.
[6] Prasad S S V, Tilak K V B R, Gollakota K G. Role of Bacillus thuringiensis var. thuringiensis on the larval survivability and egg hatching of Meloidogyne spp., the causative agent of root-knot disease[J]. Journal of Invertebrate Pathology, 1972, 20(3):377-378.
[7] Ignoff C M, Dropin V H. Deleterious effects of the thermostable toxin of Bacillus thuringiensis on species of soil-inhabiting, myceliophagus and plant-parasitic nematodes[J]. Journal of the Kansas Entomological Society, 1977, 50(3):394-398.
[8] Bone L W, Bottjer K P, Gill S S. Trichostrongylus colubriformis:egg lethality due to Bacillus thuringiensis crystal toxin[J]. Experimental Parasitology, 1985, 60(3):314-322.
[9] Bottjer K P, Bone L W, Gill S S. Nematoda:susceptibility of the egg to Bacillus thuringiensis toxins[J]. Experimental Parasitology, 1985, 60(2):239-244.
[10] Bradfish G A. Pressure filter[P]. World Patent, NO CA92121. 1992.
[11] Sharma R D. Biological control of the soybean cyst nematode Heterodera glycines[J]. Documentors-EMBRAPA, Centro de Pesquisa Agropecuaria do Oeste, 1997, 12:26-31.
[12] 姚宝安, 王乾兰, 赵俊龙, 等. 苏云金芽胞杆菌伴胞晶体对乳突类圆线虫丝虫型幼虫的杀灭作用[J]. 中国兽医科技, 1995, 25(6):39.
[13] 王祥, 姚宝安. 苏云金芽胞杆菌的伴胞晶体毒素对捻转血矛线虫第2期幼虫的毒力[J]. 华中农业大学学报, 1996, 15(4):359-361.
[14] 冯汉利, 姚宝安, 周艳琴, 等. 苏云金芽胞杆菌伴胞晶体蛋白对猪体内蛔虫的作用及其血液生理生化指标的分析[J]. 中国兽医学报, 2007, 27(4):523-526.
[15] 余子全, 白培胜, 郭素霞, 等. 苏云金芽胞杆菌杀线虫伴胞晶体蛋白基因cry6Aa的克隆与表达[J]. 微生物学报, 2007, 47(5):865-868.
[16] 徐华潮, 徐金华, 张立钦, 等. 苏云金芽孢杆菌对松材线虫的杀线活性[J]. 中国生物防治, 2010, 26(1):85-89.
[17] 雷梦英. 苏云金芽孢杆菌cry5Ba3基因的表达及对松材线虫的毒杀效果研究[D]. 杭州:浙江农林大学, 2015.
[18] 周勇. 苏云金芽胞杆菌BRC_XQ12菌株挥发性物质对松材线虫的作用[D]. 福州:福建农林大学, 2012.
[19] 张群林. 源于地衣的苏云金芽胞杆菌分离及对广州管圆线虫生测和活性因子的研究[D]. 福州:福建农林大学, 2011.
[20] Hu Y, Aroian R V. Bacterial pore-forming proteins as anthelmintics[J]. Invertebrate Neuroscience, 2012, 12(1):37-41.
[21] Hu Y, Zhan B, Keegan B, et al. Mechanistic and single-dose in vivo therapeutic studies of Cry5B anthelmintic action against hookworms[J]. PLoS Neglected Tropical Diseases, 2012, 6(11):e1900.
[22] Urban J F Jr, Hu Y, Miller M M, et al. Bacillus thuringiensis-derived Cry5B has potent anthelmintic activity against Ascaris suum[J]. PLoS Neglected Tropical Diseases, 2013, 7(6):e2263.
[23] Cheng F, Wang J, Song Z, et al. Complete genome sequence of Bacillus thuringiensis YC-10, a novel active strain against plant-parasitic nematodes[J]. Journal of Biotechnology, 2015, 210:17-18.
[24] 余子全, 刘斌, 邹雪, 等. 苏云金芽孢杆菌晶体蛋白对北方根结线虫生物测定方法的建立和杀虫效果评价[J]. 植物病理学报, 2008, 38(2):219-224.
[25] http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/toxins2.html.
[26] Guo S, Liu M, Peng D, et al. New strategy for isolating novel nematicidal crystal protein genes from Bacillus thuringiensis strain YBT-1518[J]. Applied and Environmental Microbiology, 2008, 74(22):6997-7001.
[27] Iatsenko I, Boichenko I, Sommer R J. Bacillus thuringiensis DB27 produces two novel protoxins, Cry21Fa1 and Cry21Ha1, which act synergistically against nematodes[J]. Applied and Environmental Microbiology, 2014, 80(10):3266-3275.
[28] Schnepf H E, Lee S, Dojillo J, et al. Characterization of Cry34/Cry35 binary insecticidal proteins from diverse Bacillus thuringiensis strain collections[J]. Applied and Environmental Microbiology, 2005, 71(4):1765-1774.
[29] Wei J Z, Hale K, Carta L, et al. Bacillus thuringiensis crystal proteins that target nematodes[J]. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(5):2760-2765.
[30] Salehi J G, Seifinejad A, Saeedizadeh A, et al. Molecular detection of nematicidal crystalliferous Bacillus thuringiensis strains of Iran and evaluation of their toxicity on free-living and plant-parasitic nematodes[J]. Canadian Journal of Microbiology, 2008, 54(10):812-822.
[31] Peng D, Chai L, Wang F, et al. Synergistic activity between Bacillus thuringiensis Cry6Aa and Cry55Aa toxins against Meloidogyne incognita[J]. Microbial Biotechnology, 2011, 4(6):794-798.
[32] Wang P, Zhang C, Guo M, et al. Complete genome sequence of Bacillus thuringiensis YBT-1518, a typical strain with high toxicity to nematodes[J]. Journal of Biotechnology, 2014, 171(1):1-2.
[33] Iatsenko I, Nikolov A, Sommer R J. Identification of distinct Bacillus thuringiensis 4A4 nematicidal factors using the model nematodes Pristionchus pacificus and Caenorhabditis elegans[J]. Toxins, 2014, 6(7):2050-2063.
[34] Masri L, Branca A, Sheppard A E, et al. Host-pathogen coevolution:the selective advantage of Bacillus thuringiensis virulence and its Cry toxin genes[J]. PLoS Biology, 2015, 13(6):e1002169.
[35] Sheppard A E, Nakad R, Saebelfeld M, et al. High instability of a nematicidal Cry toxin plasmid in Bacillus thuringiensis[J]. Journal of Invertebrate Pathology, 2016, 133(1):34-40.
[36] Sharma C B, Prasad S S, Pai S B, et al. The exotoxin of Bacillus thuringiensis:a new C-mitotic agent[J]. Experientia, 1976, 32(11):1465-1466.
[37] Liu X, Ruan L, Hu Z, et al. Genome-wide screening reveals the genetic determinants of an antibiotic insecticide in Bacillus thuringiensis[J]. The Journal of Biological Chemistry, 2010, 285(50):39191-39200.
[38] Liu X, Ruan L, Peng D, et al. Thuringiensin:a thermostable secondary metabolite from Bacillus thuringiensis with insecticidal activity against a wide range of insects[J]. Toxins, 2014, 6(8):2229-2238.
[39] Du C, Cao S, Shi X, et al. Genetic and biochemical characterization of a gene operon for trans-aconitic acid, a novel nematicide from Bacillus thuringiensis[J]. The Journal of Biological Chemistry, 2017, 292(8):3517-3530.
[40] Luo X, Chen L, Huang Q, et al. Bacillus thuringiensis metalloproteinase Bmp1 functions as a nematicidal virulence factor[J]. Applied and Environmental Microbiology, 2013, 79(2):460-468.
[41] Peng D, Lin J, Huang Q, et al. A novel metalloproteinase virulence factor is involved in Bacillus thuringiensis pathogenesis in nematodes and insects[J]. Environmental Microbiology, 2016, 18(3):846-862.
[42] Ni H, Zeng S, Qin X, et al. Molecular docking and site-directed mutagenesis of a Bacillus thuringiensis chitinase to improve chitinolytic, synergistic lepidopteran-larvicidal and nematicidal activities[J]. International Journal of Biological Sciences, 2015, 11(3):304-315.
[43] Ruan L, Wang H, Cai G, et al. A two-domain protein triggers heat shock pathway and necrosis pathway both in model plant and nematode[J]. Environmental Microbiology, 2015, 17(11):4547-4565.
[44] Zheng Z, Zheng J, Zhang Z, et al. Nematicidal spore-forming Bacilli share similar virulence factors and mechanisms[J]. Scientific Report, 2016, 6:31341.
[45] Griffitts J S, Haslam S M, Yang T, et al. Glycolipids as receptors for Bacillus thuringiensis crystal toxin[J]. Science, 2005, 307(5711):922-925.
[46] Zhang F, Peng D, Cheng C, et al. Bacillus thuringiensis crystal protein Cry6Aa triggers caenorhabditis elegans necrosis pathway mediated by aspartic protease (ASP-1)[J]. PLoS Pathogens, 2016, 12(1):e1005389.
[47] Kho M F, Bellier A, Balasubramani V, et al. The pore-forming protein Cry5B elicits the pathogenicity of Bacillus sp. against Caenorhabditis elegans[J]. PLoS ONE, 2011, 6(12):e29122.
[48] Wang F, Liu Y, Zhang F, et al. Improvement of crystal solubility and increasing toxicity against Caenorhabditis elegans by asparagine substitution in block 3 of Bacillus thuringiensis crystal protein Cry5Ba[J]. Applied and Environmental Microbiology, 2012, 78(20):7197-7204.
[49] Zhang F, Peng D, Ye X, et al. In vitro uptake of 140 kDa Bacillus thuringiensis nematicidal crystal proteins by the second stage juvenile of Meloidogyne hapla[J]. PLoS ONE, 2012, 7(6):e38534.
[50] Crickmore N. Using worms to better understand how Bacillus thuringiensis kill inscts[J]. Trends in Microbiology, 2005, 13(8):347-350.
[51] Griffitts J S, Aroian R V. Many roads to resistance:how invertebrates adapt to Bt toxins[J]. Bioessays, 2005, 27(6):614-624.
[52] Bottjer K P, Bone L W, Gill S S. Nematoda:susceptibility of the egg to Bacillus thuringiensis toxins[J]. Experimental Parasitology, 1985, 60(2):239-244.
[53] Marroquin L D, Elyassnia D, Griffitts J S, et al. Bacillus thuringiensis (Bt) toxin susceptibility and isolation of resistance mutants in the nematode Caenorhabditis elegans[J]. Genetics, 2000, 155(4):1693-1699.
[54] Wei J Z, Hale K, Carta L, et al. Bacillus thuringiensis crystal proteins that target nematodes[J]. Proceedings of the National Academy of Sciences, 2003, 100(5):2760-2765.
[55] Griffitts J S, Haslam S M, Yang T, et al. Glycolipids as receptors for Bacillus thuringiensis crystal toxin[J]. Science, 2005, 307(5711):922-925.
[56] Dementiev A, Board J, Sitaram A, et al. The pesticidal Cry6Aa toxin from Bacillus thuringiensis is structurally similar to HlyE-family alpha pore-forming toxins[J]. BMC Biology, 2016, 14(1):71.
[57] Beebee T, Korner A, Bond R P. Differential inhibition of mammalian ribonucleic acid polymerases by an exotoxin from Bacillus thuringiensis. The direct observation of nucleoplasmic ribonucleic acid polymerase activity in intact nuclei[J]. Biochemical Journal, 1972, 127(4):619-634.