水稻拟禾本科根结线虫生防细菌的分离鉴定与防治效果评价

doi:10.16409/j.cnki.2095-039x.2018.04.016

中国生物防治学报 ›› 2018, Vol. 34 ›› Issue (4): 606-615.DOI: 10.16409/j.cnki.2095-039x.2018.04.016

水稻拟禾本科根结线虫生防细菌的分离鉴定与防治效果评价

姚思敏^1,2, 丁中^1,2, 周建宇^1,2, 袁涛^1,2, 黄勇椿^1,2, 叶姗¹

1. 湖南农业大学植物保护学院/湖南省生物农药与制剂加工工程技术研究中心, 长沙 410128;
2. 南方粮油作物协同创新中心, 长沙 410128

收稿日期:2017-12-26 出版日期:2018-08-08 发布日期:2018-07-30
通讯作者: 叶姗,博士,讲师,E-mail:756036807@qq
作者简介:姚思敏,硕士研究生,E-mail:1020107080@qq.com
基金资助:
公益性行业（农业）科研专项（201503114）

Isolation and Identification of Biocontrol Bacterium and Its Control Effect against Meloidogyne graminicola

YAO Simin^1,2, DING Zhong^1,2, ZHOU Jianyu^1,2, YUAN Tao^1,2, HUANG Yongchun^1,2, YE Shan¹

1. Hunan Provincial Engineering & Technology Research Center for Bio-pesticide and Formulation Processing/College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;
2. Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha 410128, China

Received:2017-12-26 Online:2018-08-08 Published:2018-07-30

摘要/Abstract

摘要： 为获得对水稻拟禾本科根结线虫具有较高杀线虫活性的生防细菌，初筛以马铃薯腐烂茎线虫、复筛以拟禾本科根结线虫2龄幼虫为靶标线虫，采用直接触杀法对土壤分离细菌进行筛选。获得8株具有较高杀线虫活性的菌株，其中D45菌株发酵液的杀虫活性最好。其发酵液上清5倍稀释液处理24、48 h后，马铃薯腐烂茎线虫和拟禾本科根结线虫2龄幼虫的死亡率分别为69.8%、72.1%和60.9%、70.4%。盆栽试验和田间试验表明，在拟禾本科根结线虫侵染前采用D45菌株发酵液进行土壤处理，可显著减少水稻根结数。D45高活性菌株产生的杀线虫物质对蛋白酶稳定，但不耐热和酸。经生理生化测定结合16S rRNA基因序列分析，D45菌株为巨大芽胞杆菌Bacillus megaterium。D45菌株对防治水稻拟禾本科根结线虫具有应用潜力。

关键词: 巨大芽胞杆菌, 生物防治, 拟禾本科根结线虫, 活性产物

Abstract: In order to obtain biocontrol bacteria which can efficiently control Meloidogyne graminicola, soil bacteria were isolated and screened in vitro for their effects on mortality of Ditylenchus destructor and M. graminicola. From primary screening against D. destructor and second screening against second-stage juveniles of M. graminicola, 8 soil bacteria secreting high nematocidal metabolites were isolated. Among them, strain D45 showed the highest activity. After 24 h and 48 h incubation, the supernatant of strain D45 culture diluted five times resulted in a mortality of 69.8% and 72.1% to D. destructor, 60.9% and 70.4% to M. graminicola, respectively. Pot and field experiments indicated that soil drenching with fermentation broth of strain D45 prior to nematode inoculation greatly reduced the numbers of galls. The nematicidal substances of strain D45 were sensitive to protease,while insensitive to acid and heating. Based on physiological and biochemical characters, 16S rRNA gene sequence analysis, D45 was identified to be Bacillus megaterium. This study demonstrates that B. megaterium D45 has good application potential in controlling of M. graminicola.

Key words: Bacillus megaterium, biocontrol, Meloidogyne graminicola, bioactive metabolites

中图分类号:

S476

姚思敏, 丁中, 周建宇, 袁涛, 黄勇椿, 叶姗. 水稻拟禾本科根结线虫生防细菌的分离鉴定与防治效果评价[J]. 中国生物防治学报, 2018, 34(4): 606-615.

YAO Simin, DING Zhong, ZHOU Jianyu, YUAN Tao, HUANG Yongchun, YE Shan. Isolation and Identification of Biocontrol Bacterium and Its Control Effect against Meloidogyne graminicola[J]. journal1, 2018, 34(4): 606-615.

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水稻拟禾本科根结线虫生防细菌的分离鉴定与防治效果评价

Isolation and Identification of Biocontrol Bacterium and Its Control Effect against Meloidogyne graminicola

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