水稻细菌性条斑病生防菌株筛选和田间防治效果

doi:10.16409/j.cnki.2095-039x.2019.04.022

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (4): 648-654.DOI: 10.16409/j.cnki.2095-039x.2019.04.022

水稻细菌性条斑病生防菌株筛选和田间防治效果

王星¹, 杨俊¹, 曹卫华¹, 魏兰芳², 姬广海¹

1. 云南农业大学/农业生物多样性与病虫害控制教育部重点实验室, 昆明 650201;
2. 云南农业大学农科基础实验教学中心, 昆明 650201

收稿日期:2018-10-10 出版日期:2019-08-08 发布日期:2019-08-10
通讯作者: 姬广海,教授,E-mail:550356818@qq.com。
作者简介:王星,硕士研究生,E-mail:631008845@qq.com;杨俊,博士研究生,E-mail:729747016@qq.com
基金资助:
云南省科技厅农业基础研究联合专项（2017FG001-005）；国家重点研发计划（2018YFD0200308，2017YFC1702502，2018YFD0200703）；云南省科技厅重点研发（农业领域）项目（2018BB016）；国家自然科学基金（31460458）

Screening of Biocontrol Bacteria on Rice Bacteria Leaf Streak and Determination of Field Control Efficacy

WANG Xing¹, YANG Jun¹, CAO Weihua¹, WEI Lanfang², JI Guanghai¹

1. Key Laboratory of Agriculture Biodiversity for Plant Disease Management, the Ministry of Education/Yunnan Agricultural University, Kunming 650201, China;
2. Agricultural Foundation Experiment Teaching Center, Yunnan Agricultural University, Kunming 650201, China

Received:2018-10-10 Online:2019-08-08 Published:2019-08-10

摘要/Abstract

摘要： 为筛选防治水稻细菌性条斑病的生防菌株，通过平板对峙试验、温室及田间试验，测定了4个菌株对水稻促生和防治细菌性条斑病的效果。通过平板对峙试验明确了4株生防菌株抑菌活性；温室试验中，除生防菌LH外，其他3株生防菌的发酵液处理后的水稻幼苗的株高、根系、鲜重均显著高于对照。接种结果显示接种病原菌前喷施生防菌防效最好。生防菌L1在3个不同试验田中防效均达到55%以上，同时增产效果达到10%以上，显著高于其他处理。研究表明，生防菌L1不仅可以用于水稻细菌性条斑病的生物防治，而且可以促进水稻生长，具有较好的生产应用潜力。

关键词: 水稻细菌性条斑病, 生防细菌, 促生, 生物防治

Abstract: In order to screen better biocontrol bacteria against rice bacteria leaf streak (BLS) caused by Xanthomonas oryzae pv.oryzicola, the biocontrol efficacy of four biocontrol bacteria strains on rice bacteria leaf streak were evaluated by plate confrontation test, greenhouse and field experiments. In the plate confrontation test, the antibacterial activities of the four biocontrol bacteria strains were determined. Greenhouse experiment showed that except for biocontrol strain LH, the plant height, root length and fresh weight of the rice seedlings treated with the other three biocontrol bacteria fermentation broths were significantly higher than those of the control. Inoculation test indicated that application of biocontrol bacteria before inoculation of pathogenic X. oryzae had the best control effect. Field trials demonstrated that the biocontrol strain L1 achieved more than 55% control efficacy in three different fields, and the yield increase rate was more than 10%, which was significantly higher than other treatments. This study shows that the biocontrol strain L1 can not only be used for the biological control of the BLS disease, but also promote rice growth, thus it has a good potential application.

Key words: rice bacteria leaf streak, biocontrol bacteria, plant growth promotion, biological control

中图分类号:

S476

王星, 杨俊, 曹卫华, 魏兰芳, 姬广海. 水稻细菌性条斑病生防菌株筛选和田间防治效果[J]. 中国生物防治学报, 2019, 35(4): 648-654.

WANG Xing, YANG Jun, CAO Weihua, WEI Lanfang, JI Guanghai. Screening of Biocontrol Bacteria on Rice Bacteria Leaf Streak and Determination of Field Control Efficacy[J]. Chinese Journal Of Biological Control, 2019, 35(4): 648-654.

参考文献

[1] Hummel A W, Wilkins K E, Wang L, et al. A transcription activator-like effector from Xanthomonas oryzae pv. oryzicola elicits dose-dependent resistance in rice[J]. Molecular Plant Pathology, 2016, 18(1):55-66.
[2] Niñoliu D O, Ronald P C, Bogdanove A J. Xanthomonas oryzae pathovars:model pathogens of a model crop[J]. Molecular Plant Pathology, 2010, 7(5):303-324.
[3] 周丽洪, 韩阳, 李淼, 等. 西南地区水稻细菌性条斑病菌链霉素抗性研究[J]. 云南农业大学学报, 2014, 29(5):654-660.
[4] Idris E S E, Iglesias D J, Talon M, et al. Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42[J]. Molecular Plant-Microbe Interactions, 2007, 20(6):619.
[5] 董国菊, 李文英, 刘翠平, 等. 烟草疫霉拮抗菌株P-72-10的鉴定及其拮抗代谢产物初步分析[J]. 植物病理学报, 2012, 42(3):297-305.
[6] 彭兵, 张树斌, 贾宇, 等. 枯草芽孢杆菌菌株A抗菌蛋白的分离纯化及抗真菌机理[J]. 中国农业科学, 2011, 44(1):67-74.
[7] Folman L B, Postma J, van Veen J A. Characterisation of Lysobacter enzymogenes (Christensen and Cook 1978) strain 3.1T8, a powerful antagonist of fungal diseases of cucumber[J]. Microbiological Research, 2003, 158(2):107-115.
[8] Kobayashi D Yuen G. The potential of Lysobacter spp. as bacterial biological control agents for plant diseases[J]. CAB Review:Perspectives in Agriculture Veterinary Science Nutrition and Natural Resources, 2007, 2(7):1-11.
[9] Luo C, Liu X, Zhou H, et al. Nonribosomal peptide synthase gene clusters for lipopeptide biosynthesis in Bacillus subtilis 916 and their phenotypic functions[J]. Applied and Environmental Microbiology, 2015, 81(1):422-431.
[10] 张淼, 周立梅, 徐德阳, 等. 侧孢短芽孢杆菌BL-21抗菌蛋白的稳定性分析和分离纯化[J]. 中国农学通报, 2017, 33(15):43-48.
[11] 李红晓, 张殿朋, 赵洪新, 等. 解淀粉芽胞杆菌MH71抗菌物质理化特性及对番茄灰霉病菌的抑菌活性[J]. 中国生物防治学报, 2016, 32(4):485-492.
[12] Zhang R S, Liu Y F, Luo C P, et al. Bacillus amyloliquefaciens Lx-11, a potential biocontrol agent against rice bacterial leaf streak[J]. Journal of Plant Pathology, 2012, 94(3):609-619.
[13] Jetiyanon K, Wei G, Tuzun S, et al. Induced systemic resistance (ISR) of cucumber by stem jection and seed treatment with PGPR[J]. Phytopathology, 1995, 85:114.
[14] 陈志谊, 许志刚, 陆凡, 等. 拮抗细菌B-916对水稻植株的抗性诱导作用[J]. 西南农业学报, 2001, 14(2):44-48.
[15] 李德全, 陈志谊, 聂亚锋. 生防菌Bs-916及高效突变菌株抗菌物质及其对水稻抗性诱导作用的研究[J]. 植物病理学报, 2008, 38(2):192-198.
[16] 陈思宇. 水稻纹枯病菌拮抗细菌的筛选与防治作用研究[D]. 南京:南京农业大学, 2013.
[17] 王卉, 尚庆茂, 张志刚, 等. 解淀粉芽孢杆菌L-H15产促生物质分析及发酵工艺优化[J]. 食品科学, 2017, 38(10):74-81.
[18] 闫志宇, 翟蓓蓓, 李术娜, 等. 乙草胺降解菌L3的促生物质[J]. 江苏农业科学, 2017, 45(22):295-298.
[19] 杨晓云, 陈志谊, 蒋盼盼, 等. 解淀粉芽孢杆菌B1619对番茄的促生作用[J]. 中国生物防治学报, 2016, 32(3):349-356.
[20] Hyunsun K, Jin R D, Krishnan H B, et al. Biocontrol ability of Lysobacter antibioticus HS124 against Phytophthora blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes[J]. Current Microbiology, 2009, 59(6):608-615.
[21] Lou L, Qian G, Xie Y, et al. Biosynthesis of HSAF, a tetramic acid-containing macrolactam from Lysobacter enzymogenes[J]. Journal of the American Chemical Society, 2011, 133(4):643-645.
[22] 王皓楠, 靳鹏飞, 康迅, 等. 解淀粉芽孢杆菌HAB-6抑菌物质及其相关基因的分析[J]. 江苏农业科学, 2018, 46(4):79-83.
[23] Zhao Y, Qian G, Ye Y, et al. Heterocyclic aromatic N-oxidation in the biosynthesis of phenazine antibiotics from Lysobacter antibioticus[J]. Organic Letters, 2016, 18(10):2495-2498.
[24] Laborda P, Zhao Y, Ling J, et al. Production of antifungal p-aminobenzoic acid in Lysobacter antibioticus OH13[J]. Journal of Agricultural and Food Chemistry, 2018, 66(3):630.
[25] Gardener M S, Kim I S, Kim K Y, et al. Draft genome sequence of a chitinase-producing biocontrol bacterium, Lysobacter antibioticus HS124[J]. Plant Disease, 2014, 20(3):216-218.

水稻细菌性条斑病生防菌株筛选和田间防治效果

Screening of Biocontrol Bacteria on Rice Bacteria Leaf Streak and Determination of Field Control Efficacy

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

联系我们

[1]	阿尔孜姑丽·肉孜, 吐尔逊·阿合买提, 付开赟, 丁新华, 阿地力·沙塔尔, 郭文超. 新疆玉米种植区瓢虫资源调查及多样性分析[J]. 中国生物防治学报, 2020, 36(5): 697-707.
[2]	孙元星, 郝亚楠, 李明凌. 贮藏前补充人工饲料对七星瓢虫低温耐受力的影响[J]. 中国生物防治学报, 2020, 36(5): 708-713.
[3]	许帅, 谢学文, 张昀, 石延霞, 柴阿丽, 李磊, 李宝聚. 马铃薯枯萎病生防芽胞杆菌筛选及生防效果研究[J]. 中国生物防治学报, 2020, 36(5): 761-770.
[4]	郭义, 赵灿, 李君摘, 李敦松. 蠋蝽对荔枝蝽一龄若虫的捕食功能反应[J]. 中国生物防治学报, 2020, 36(5): 826-831.
[5]	田俊策, 鲁艳辉, 王国荣, 郑许松, 杨亚军, 徐红星, 方琦, 叶恭银, 臧连生, 吕仲贤. 种赤眼蜂对草地贪夜蛾卵的寄生能力研究[J]. 中国生物防治学报, 2020, 36(4): 485-490.
[6]	路子云, 杨小凡, 马爱红, 冉红凡, 刘文旭, 李建成. 管侧沟茧蜂对不同日龄草地贪夜蛾幼虫的寄生效果[J]. 中国生物防治学报, 2020, 36(4): 491-495.
[7]	杨磊, 李芬, 吴少英. 草地贪夜蛾寄生蜂资源及其调控寄主免疫反应的研究[J]. 中国生物防治学报, 2020, 36(4): 496-506.
[8]	黄潮龙, 汤印, 何康来, 王振营. 双斑青步甲幼虫对草地贪夜蛾幼虫的捕食能力[J]. 中国生物防治学报, 2020, 36(4): 507-512.
[9]	李萍, 李玉艳, 向梅, 王孟卿, 毛建军, 陈红印, 张礼生. 大草蛉幼虫对草地贪夜蛾低龄幼虫的捕食能力评价[J]. 中国生物防治学报, 2020, 36(4): 513-519.
[10]	王亚楠, 赵胜园, 何运转, 吴孔明, 李国平, 封洪强. 黄带犀猎蝽对草地贪夜蛾幼虫的捕食作用[J]. 中国生物防治学报, 2020, 36(4): 525-529.
[11]	罗梅, 罗玉霖, 陈沫冰, 舒永馨, 陈欣瑜, 董章勇. 拟康宁木霉Tk1的分离鉴定、拮抗作用及其生物学特性[J]. 中国生物防治学报, 2020, 36(4): 581-586.
[12]	赵玳琳, 何海永, 吴石平, 陈小均, 谭清群, 杨学辉. 棘孢木霉GYSW-6m1对草莓炭疽病的生防机制及其防病促生作用研究[J]. 中国生物防治学报, 2020, 36(4): 587-595.
[13]	潘洪生, 李号宾, 丁瑞丰, 李海强, 王冬梅, 阿克旦·吾外士, 刘建. 多异瓢虫对棉黑蚜的捕食能力[J]. 中国生物防治学报, 2020, 36(4): 628-631.
[14]	廖平, 石新如, 郭义, 殷焱芳, 朱艳娟, 李玉艳, 毛建军, 王孟卿, 张礼生, 陈红印, 刘晨曦. 低温饲养对蠋蝽生长发育的影响[J]. 中国生物防治学报, 2020, 36(3): 340-346.
[15]	李磊, 赵昱榕, 郑斐, 石延霞, 柴阿丽, 谢学文, 李宝聚. 芹菜软腐病拮抗芽胞杆菌筛选及防治效果[J]. 中国生物防治学报, 2020, 36(3): 388-395.