链霉菌3-10发酵液及提取物的稳定性研究

doi:10.16409/j.cnki.2095-039x.2021.08.007

中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (1): 250-257.DOI: 10.16409/j.cnki.2095-039x.2021.08.007

链霉菌3-10发酵液及提取物的稳定性研究

吕昂^1,2, 吴明德³, 张静³, 杨龙³, 李国庆³

1. 湖北省农业科学院农业质量标准与检测技术研究所, 武汉 430064;
2. 农产品营养质量与安全湖北省重点实验室, 武汉 430064;
3. 华中农业大学植物科学与技术学院, 武汉 430070

收稿日期:2021-07-19 出版日期:2022-02-08 发布日期:2022-03-01
通讯作者: 李国庆,博士,教授,E-mail:guoqingli@mail.hzau.edu.cn
作者简介:吕昂,博士,助理研究员,E-mail:lvang2007@foxmail.com
基金资助:
国家油菜产业技术体系（CARS-12）；湖北省科技厅项目（2018CFC850）

Stability of Cultural Filtrate and Crude Extract Produced by Streptomyces sp. 3-10

LÜ Ang^1,2, WU Mingde³, ZHANG Jing³, YANG Long³, LI Guoqing³

1. Institute of Quality Standard and Testing Technology for Agro-products, Hubei Academy of Agricultural Science, Wuhan 430064, China;
2. Hubei Key Laboratory of Nutritional Quality and Safety of Agro-products, Wuhan 430064, China;
3. College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2021-07-19 Online:2022-02-08 Published:2022-03-01

摘要/Abstract

摘要： 为合理利用链霉菌3-10的代谢产物，本研究采用不同极性的有机溶剂提取链霉菌3-10发酵液中的抗真菌物质并得到发酵液的提取物。在此基础上测定了温度、紫外线辐射、酸碱度和贮存条件对3-10抗真菌物质稳定性的影响。结果表明，乙酸乙酯为最佳提取溶剂，链霉菌3-10发酵液的乙酸乙酯提取物对灰霉病菌菌丝生长的抑制作用最强，提取后余液几乎无抑真菌活性。该提取物对80℃以上的高温比较敏感，紫外线照射1 min后提取物的抗真菌活性开始显著下降，照射5 min后抗真菌活性丧失。在酸性条件下（pH=2~6）提取物抗真菌活性较高，碱性条件（pH=8~13）会破坏提取物的抗真菌活性。提取物经冷冻干燥后制备的干粉在低温（4℃）和室温（20℃~28℃）条件下贮存100 d仍能保持较强生物活性。链霉菌3-10发酵液和提取物的稳定性和贮存条件的研究可为后续开发利用链霉菌3-10提供理论依据和数据支持。

关键词: 链霉菌3-10, 提取物, 稳定性, 贮存条件, 抗真菌活性

Abstract: In order to better utilize the secondary metabolites from Streptomyces sp. 3-10, the crude extracts with antifungal activity were extracted by using different organic solvents from the cultural filtrate of Streptomyces sp. 3-10. The stability of the antifungal substance was investigated for response to temperature, pH, UV-irradiation and storage conditions. The results showed that ethyl acetate was the best organic solvent to extract the antifungal substance from the cultural filtrate, as the crude extract by ethyl acetate exhibited high antifungal activity against Botrytis cinerea. The crude extract was sensitive to temperature higher than 80℃ UV-irradiation for 1 min could compromise the antifungal activity of the crude extract, and the antifungal activity of the crude extract nearly lost after UV-irradiation for 5 min. The crude extract showed high antifungal activity in acidic condition, but lost in alkali condition. The lyophilized powder of the crude extract could maintain its antifungal activity at 4℃ and room temperature for 100 d. The stability and storage condition study of the crude extract can provide data support for future applying of Streptomyces sp. 3-10 as a biocontrol agent.

Key words: Streptomyces sp. 3-10, crude extract, stability, storage condition, antifungal activity

中图分类号:

S476

吕昂, 吴明德, 张静, 杨龙, 李国庆. 链霉菌3-10发酵液及提取物的稳定性研究[J]. 中国生物防治学报, 2022, 38(1): 250-257.

LÜ Ang, WU Mingde, ZHANG Jing, YANG Long, LI Guoqing. Stability of Cultural Filtrate and Crude Extract Produced by Streptomyces sp. 3-10[J]. Chinese Journal of Biological Control, 2022, 38(1): 250-257.

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链霉菌3-10发酵液及提取物的稳定性研究

Stability of Cultural Filtrate and Crude Extract Produced by Streptomyces sp. 3-10

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