全局调控子MtrAsbh影响米尔贝霉素生物合成的研究

doi:10.16409/j.cnki.2095-039x.2022.03.030

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (5): 1083-1093.DOI: 10.16409/j.cnki.2095-039x.2022.03.030

全局调控子MtrA_sbh影响米尔贝霉素生物合成的研究

祝亚杰¹, 王佳彬^1,2, 李珊珊¹, 向文胜^1,2, 张艳艳¹

1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;
2. 东北农业大学生命科学学院, 哈尔滨 150030

收稿日期:2022-05-23 出版日期:2023-10-08 发布日期:2023-10-25
通讯作者: 向文胜,教授,E-mail:xiangwensheng@neau.edu.cn;张艳艳,副研究员,E-mail:yyzhang@ippcaas.cn。
作者简介:祝亚杰,硕士研究生,E-mail:zyj_1336104280@163.com;王佳彬,博士研究生,E-mail:jbwang0725@163.com。
基金资助:
国家自然科学基金（31872936）

Effect of the Global Regulator MtrA_sbh on the Biosynthesis of Milbemycins

ZHU Yajie¹, WANG Jiabin^1,2, LI Shanshan¹, XIANG Wensheng^1,2, ZHANG Yanyan¹

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. School of Life Science, Northeast Agricultural University, Harbin 150030, China

Received:2022-05-23 Online:2023-10-08 Published:2023-10-25

摘要/Abstract

摘要： MtrA是链霉菌中保守存在的全局性应答调控蛋白。为探究冰城链霉菌中MtrA同源蛋白MtrA_sbh在生物农药米尔贝霉素产生中的作用，本文通过基因敲除、回补和过表达确定了MtrA_sbh对米尔贝霉素产生的影响；通过转录分析和凝胶阻滞初步探究了MtrA_sbh影响米尔贝霉素产生的机制；通过转录分析检测了MtrA_sbh对基因组中其他天然产物生物合成基因表达的影响。结果表明MtrA_sbh为米尔贝霉素产生的关键激活子，其失活导致米尔贝霉素彻底不产生，而过表达则显著提高米尔贝霉素的产量。MtrA_sbh通过影响米尔贝霉素基因簇和前体合成相关基因的表达来调控米尔贝霉素的产生。此外，MtrA_sbh作为激活子或抑制子差异化影响了冰城链霉菌基因组中众多天然产物生物合成基因的表达。

关键词: 全局调控子, MtrA_sbh, 冰城链霉菌, 米尔贝霉素

Abstract: MtrA is a global response regulatory protein that is highly conserved in Streptomyces. The function of MtrA from Streptomyces bingchenggensis (MtrA_sbh) in the biosynthesis of biopesticide milbemycins was studied by by gene deletion, complementation, overexpression, transcriptional analysis and gel retardation assay. Results showed that the mtrA_sbh deletion mutant displayed a complete loss of milbemycin production, while overexpression of mtrA_sbh significantly promoted the production of milbemycin, indicating that MtrA_sbh is a key activator of milbemycin biosynthesis. Our results further show that MtrA_sbh regulates milbemycin production by affecting the expression of milbemycin biosynthetic gene clusters and genes related to precursor supply. In addition, MtrA_sbhcan act as an activator or a repressor to differentially control the expression of many other natural product biosynthesis genes presented in the genome of Streptomyces bingchenggensis.

Key words: global regulator, MtrA_sbh, Streptomyces bingchenggensis, milbemycin

中图分类号:

S476.1

祝亚杰, 王佳彬, 李珊珊, 向文胜, 张艳艳. 全局调控子MtrA_sbh影响米尔贝霉素生物合成的研究[J]. 中国生物防治学报, 2023, 39(5): 1083-1093.

ZHU Yajie, WANG Jiabin, LI Shanshan, XIANG Wensheng, ZHANG Yanyan. Effect of the Global Regulator MtrA_sbh on the Biosynthesis of Milbemycins[J]. Chinese Journal of Biological Control, 2023, 39(5): 1083-1093.

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全局调控子MtrA_sbh影响米尔贝霉素生物合成的研究

Effect of the Global Regulator MtrA_sbh on the Biosynthesis of Milbemycins

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全局调控子MtrAsbh影响米尔贝霉素生物合成的研究

Effect of the Global Regulator MtrAsbh on the Biosynthesis of Milbemycins

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全局调控子MtrA_sbh影响米尔贝霉素生物合成的研究

Effect of the Global Regulator MtrA_sbh on the Biosynthesis of Milbemycins